Antinociceptive effect of extracts of Marrubium astracanicum Jacq. aerial parts

Document Type : Original Research Article


1 Department of Pharmacognosy, Faculty of Pharmacy and Persian Medicine and Pharmacy Research center, Tehran University of Medical Sciences, Tehran, Iran

2 Departments of Pharmacognosy and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

3 Departments of Pharmacology and Toxicology and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

4 Faculty of Land and Food Systems, University of British Columbia, B.C., Canada


Objective: The genus Marrubium is used for treatment of joint pain, gout, stomach-ache and colic in Iranian Traditional Medicine. Marrubium astracanicum Jacq. (M. astracanicum) is a native species in the flora of Iran. The aim of this study was to evaluate the antinociceptive properties of various extracts of aerial parts of M. astracanicum.

Materials and Methods: Antinociceptive activities of total hydroalcoholic extract (THE) and its n-hexane (non-polar) and residual partition (polar) fractions were analyzed using formalin test in mice. Morphine (5 mg/kg) and normal saline were used as positive and negative controls, respectively.

Results: Intraperitoneal administration of THE (50, 100 and 200 mg/kg), non-polar fraction (200 mg/kg) and polar fraction (100 and 200 mg/kg), 30 min before formalin injection, caused significant analgesic activity in acute phase (0-5 min after formalin injection) of formalin test (p0.05 in comparison with morphine).  In chronic phase (15–60 min after formalin injection), non-polar and polar fractions (50, 100 and 200 mg/kg) showed significant analgesic activity (p0.05 in comparison with morphine).

Conclusion: Different extracts of M. astracanicum demonstrated antinociceptive activity that support the traditional usage of Marrubium genus for the treatment of arthritis, gout and other inflammatory diseases.


Main Subjects

De Jesus RAP, Cechinel-Filho V, Oliveira AE, V Schlemper. 1999. Analysis of the antinociceptive properties of marrubiin isolated from Marrubium vulgare. Phytomedicine, 7: 111-115.
De Souza MM, de Jesus RAP, Cechinel-Filho V, Schlemper V. 1998. Analgesic profile of hydroalcoholic extract obtained from Marrubium vulgare. Phytomedicine, 5: 103-107.
Enomoto SH, Okada Y, Guvenc A,  Erdurak CS, Coskun M¸ Okuyama T. 2004. Inhibitory effect of traditional Turkish folk medicines on aldose reductase (AR) and hematological activity, and on AR inhibitory activity of Quercetin-3-O-methyl ether isolated from Cistus laurifolius L. Biol Pharm Bull, 27: 1140-1143.
Harborne AJ. 1998. Phytochemical methods: aguide to modern techniques of plant analysis, pp. 4-31, London, Chapman and Hall.
Ilda A, Tanaka Y, Mihara T, Tabata M, Honda G, Shingu T, Takeda Y, Takishi Y, Yesilada E, Sezik E, Fujita T. 1995. Marrubinones A and B, new labdane diterpenoids from Marrubium astracanicum (Labiatea). Chem Pharm Bull, 43: 1454-1457.
Jamzad Z. 2015. Flora of Iran, pp. 400-405, Tehran, Research Institute of Forests and Ragelands Publication.
Javidnia K, Miri R, Soltani M, Khosravi AR. 2007. Constituents of the essential oil of Marrubium astracanicum Jacq. from Iran. J Essent Oil Res, 19: 559-561.
Khanavi M, Delnavazi MR, Nikoui V, Ostadhadi S, Bakhtiarian A. 2012. Evaluation of analgesic effects of hydroalcoholic extract of Marrubium parviflorum by formalin test in mice. Asian J Plant Sci, 11: 96-99.
Kunduhoglu B, Pilatin S, Caliskan F. 2011. Antimicrobial screening of some medicinal plants collected from Eskisehir, Turkey. Fresen Environ Bull, 20: 945-952.
Mahdizadeh SH, Khaleghi Ghadiri M, Gorji A. 2015. Avicenna's Canon of Medicine: a review of analgesics and anti-inflammatory substances. Avicenna J Phytomed, 5: 182-202.
Meyre-Silva A, Yunes RA, Schlemper V, Campos-Buzzi F, Cechinel-Filho V. 2005. Analgesic potential of marrubiin derivatives, a bioactive diterpene present in Marrubium vulgare (Lamiaceae). Farmaco, 60: 321-326.
Meyre-Silva CH and Cechinel-Filho V. 2010. A review of the chemical and pharmacological aspects of the genus Marrubium. Curr Pharm Des, 16: 3503-3518.
Morteza-Semnani K, Saeedi M. The essential oil of Marrubium astracanicum Jacq. from Iran. 2004. J Essent Oil Res, 7: 239-242.
Mosaddegh M, Naghib F, Moazzeni H, Pirani A, Esmaeili S. 2012. Ethnobotanical survey of herbal remedies traditionally used in Kohghiluyeh va Boyer Ahmad province of Iran. J Ethnopharmacol, 141: 80-95.
Nik ZB, Mirza M. Composition of the essential oil of Marrubium astracanicum Jacq. 2003. J Essent Oil Res, 15: 342-343.
Rigano D, Grassia A, Borrelli F, Aviello G, Piozzi F, Bruno M, Arnold NA, Capasso R, Senatore F. 2006. Phytochemical and pharmacological studies on the acetonic extract of Marrubium globosum ssp. libanoticum. Planta Med, 72: 575-578.
Sahpaz S, Garbacki N, Tits M, Bailleul F. 2002. Isolation and pharmacological activity of phenylpropanoid esters from Marrubium vulgare. J Ethnopharmacol, 79: 389-392.
Tjolsen A, Berge O, Hunskaar S, Rosland JH, Hole K. 1992. The formalin test: an evaluation of the method. Pain, 51: 5-17.
Tlili N , Elfalleh W,  Hannachi H , Yahia Y , Khaldi A, Ferchichi A, Nasri N. 2013. Screening of natural antioxidants from selected medicinal plants, Int J Food Prop, 16: 1117-1126.
Yildirim AB, Karakas FP, Turker AU. 2013. In vitro antibacterial and antitumor activities of some medicinal plant extracts, growing in Turkey. Asian Pac J Trop Med, 6: 616-624.