Document Type : Original Research Article
Faculty of Medicine and Health Sciences, UNIMAS, Kota Samarahan, Sarawak, Malaysia
Faculty of Medicine MAHSA University, Bandar Saujana Putra, Jenjarom, Selangor, Malaysia
Medicinal Products Safety, Scientific Centre for Expert Evaluation of Medicinal Products, Moscow, Russia. Department of Pharmacology, Sechenov University, Moscow, Russia
Department of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
Institute of Medical Molecular Biotechnology, and Faculty of Medicine, UiTM, Sungai Buloh, Selangor, Malaysia
Department of Pharmacognosy, Sechenov University, Moscow, Russia
Objective: Modern treatment of peptic ulcers includes antibacterial and gastroprotective medications. However, current anti-ulcer drugs possess severe side effects. Therefore, all attempts to find new effective medications free from side effects are justified. Though Berberis vulgaris is a medicinal plant commonly used for the treatment of numerous disorders, gastroprotective effect of its leaf extract was not investigated before.
Materials and Methods: Gastric ulcer was modelled in Sprague-Dawley rats after treatment with B. vulgaris leaf extract containing 0.07% of alkaloids, 0.48% of flavonoids and 8.05% of tanning substances, 10 or 50 mg of dry extract/kg, changes in the stomach mucosa were assessed semi-quantitatively, and the gastric wall was evaluated for prostaglandin E2 level using ELISA and assessed histologically by calculation of the lesion index.
Results: B. vulgaris leaf extract at the dose of 50 mg/kg reduced the macroscopic ulcer score and the microscopic lesion index, increased prostaglandin E2 concentration in the gastric wall significantly higher than atropine and B. vulgaris leaf extract 10 mg/kg.
Conclusion: The gastroprotective effect of the high dose of B. vulgaris leaf extract may be due to stimulation of prostaglandin E2 secretion in the stomach, and anti-oxidative and anti-inflammatory properties of polyphenolic complex of flavonoids and tannins present in the leaves of this plant.