Zataria multiflora Boiss inhibits muscarinic receptors of incubated tracheal smooth muscle with propranolol

Jafari, Zahra; Boskabady, Mohammad Hossein; Pouraboli, Iran; Babazade, Beheshteh

doi:10.22038/ajp.2011.115

Zataria multiflora Boiss inhibits muscarinic receptors of incubated tracheal smooth muscle with propranolol

Document Type : Original Research Article

Authors

Zahra Jafari ¹

Mohammad Hossein Boskabady ¹

Iran Pouraboli ²

Beheshteh Babazade ¹

¹ Department of Physiology and Pharmaceutical Research Centre, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran

² Department of Biology, School of Sciences, Shahid Bahonar University, Kerman, I. R. Iran

10.22038/ajp.2011.115

Abstract

Objective(s): In the present study, the effect of tissue incubation with propranolol on functional antagonism of Zataria multiflora Boiss (Z. multiflora) at muscarinic receptors of tracheal smooth muscle was examined.
Materials and Methods: The effects of three concentrations of aqueous-ethanolic extract, 10 nM atropine, and saline on muscarinic receptors were tested on incubated tracheal smooth muscle with propranolol (n=5).
Results: The EC₅₀ of all concentration of the extract and atropine was significantly higher than that of saline. There was parallel right ward shift in concentration response curves obtained in the presence of all concentrations of the extract. There was not any significant difference in the maximum response and slope obtained in the presence of different concentrations of extract compared to saline. There was significant positive correlation between the concentrations and the values of EC₅₀ (p<0.001). The value of (CR-1) obtained in the presence of highest concentration of the extract was significantly higher than that of atropine (p<0.05).
Conclusion: These results indicated that functional antagonism of Z. multiflora at muscarinic receptors of tracheal smooth muscle was mainly due to b-adrenoceptor stimulatory effect of plant.

Keywords

Zataria multiflora Boiss

Labiatae

Methacholine

Anti cholinergic effect

Trachea

Guinea pig