Modulation of lung inflammation and immune markers in asthmatic rats treated by Portulaca oleracea

Document Type : Original Research Article


1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

2 Department of Biochemistry and Biophysics, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, I.R. Iran

3 Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran


Objective: Previous studies indicated anti-inflammatory effects for Portulaca oleracea in various inflammatory disorders. In this study, the effects of P. oleracea on bronchoalveolar lavage fluid (BALF) levels of total protein (TP), Phospholipase A2(PLA2) and IgE in sensitized rats were examined.
Materials and Methods: Male rats were randomly divided into six groups namely, control (group C), sensitized rats (group S), sensitized animal treated with three concentrations of the extract of P. oleracea and dexamethasone (n = 8 for each group). The levels of TP, PLA2 and IgE in BALF were measured.
Results:The levels of TP, PLA2and IgE were significantly increased in the BALF of group S compared to group C (pand IgE compared to group S (pand IgE (p
Conclusion: The results indicated anti-inflammatory and immunomodulatory effects of P. oleracea in sensitized rats (as an animal model of asthma) which was equal or more marked than dexamethasone at studied concentrations.


Main Subjects

Abdel Moneim AE, Dkhil MA, Al-Quraishy S. 2013. The potential role of Portulaca oleracea as a neuroprotective agent in rotenone-induced neurotoxicity and apoptosis in the brain of rats. Pestic Biochem Physiol, 105: 203-212.
Ahmad Al Obaidi AH, Mohamed Al Samarai AG, Yahya Al Samarai AK, Al Janabi JM. 2008. The predictive value of IgE as biomarker in asthma. J Asthma, 45: 654-663.
Arruda SF, Siqueira EM, Souza EM. 2004. Malanga (Xanthosoma sagittifolium) and purslane (Portulaca oleracea) leaves reduce oxidative stress in vitamin A-deficient rats. Ann Nutr Metab, 48: 288-295.
Askari VR, Rezaei SA, Abnus K, Iranshahi M, Boskabady MH. 2016. The Influence of Hydro-Ethanolic Extract of Portulaca oleracea on Th1/Th2 balance in Isolated Human Lymphocytes. J Ethnopharmacol, 194: 1112-1121.                                                                              
Banerjee G, Mukherjee A. 2002. Biological activity of a common weed: Portulaca oleracea L.-II. Antifungal activity. Acta Botanica Hungarica, 44: 205-208.
Banerjee G, Mukherjee A. 2003. Antibacterial activity of a common weed, Portulaca oleracea L. Geobios (Jodhpur), 30: 143-144.
Boroushaki MT, Boskabady MH, Malek F. 2010. Antitussive effect of Portulaca oleracea L. in guinea pigs. Iran J Pharm Res, 3: 187-190.
Boskabady MH, Boroushaki M and Aslani MR. 2004. Relaxant effect of Portulaca oleraceae on guinea pig tracheal chainsand its possible mechanism (s) of action. Med Hypotheses Res, 1: 139-147.
Boskabady MH, Hashemzehi M,  Khazdair MR,  Askari VR. 2016. Hydro-ethanolic Extract of Portulaca oleracea Affects Beta-adrenoceptors of Guinea Pig Tracheal Smooth Muscle. Iran J Pharm Res, 15: 867–874. 
Boskabady MH, Tabatabaee A, Jalali S. 2014. Potential effect of the extract of Zataria multiflora and its constituent, carvacrol, on lung pathology, total and differential WBC, IgE and eosinophil peroxidase levels in sensitized guinea pigs. J Funct Foods, 11: 49–61.
Busse W, Banks-Schlegel SP, Larson GL. 1995. Childhood versus adult-onset asthma. Am J Respir Crit Care Med, 151: 1635-1639.
Chan K, Islam MW, Kamil M, Radhakrishnan R, Zakaria MNM, Habibullah M, et al. 2000. The analgesic and anti-inflammatory effects of Portulaca oleracea L. subs. sativa (Haw.) Celak. J Ethnopharmacol, 73: 445-451.
Dong F, Wang C, Duan J, Zhang W, Xiang D, Li M. 2014. Puerarin Attenuates Ovalbumin-Induced Lung Inflammation and Hemostatic Unbalance in Rat Asthma Model. eCAM, 2014: 1-9.
Hashemzehi M, Khazdair MR, Kiyanmehr M, Askari VR, Boskabady MH. 2016. Portulaca oleracea Affects Muscarinic Receptors of Guinea Pig Tracheal Smooth Muscle. Indian J Pharm Sci, 78: 388-394.
Islam MW, Zakaria MNM, Radhakrishnan R, Habibullah M, Chan K. 1998. Evaluation of analgesic activity of the aerial parts of Portulaca oleracea v. sativa and its comparison with two related spices. J Pharm Pharmacol, 50: 226-230.
Kashima N, Nakajima H, Fukaura A, et al. 1993. Study of serum phosholipase A2 activity in bronchial asthmatic patients. Arerugi, 42: 723-727.
Kelly CA, Ward C, Stenton SC, et al. 1998. Numbers and activity of cells obtained at bronchoalveolar lavage in asthma, and their relationship to airway responsiveness. Thorax, 43: 684-692.
Keyhanmanesh R, Boskabady MH, Khamneh S, Ebrahimi MA. 2009. The effect of thymoquinone, the main constituent of Nigella sativa on tracheal responsiveness and WBC count in lung lavage of sensitized guinea-pigs. Planta Med, 75: 1–5.
Keyhanmanesh R, Boskabady MH, Khamneh S, Ebrahimi MA. 2010. The effect of thymoquinone, the main constituent of Nigella sativa on tracheal responsiveness and WBC count in lung lavage of sensitized guinea-pigs. Planta Med, 76: 218–222.
Kianmehr M, Haghmorad D, Nosratabadi R, Rezaei A, Alavinezhad A, Boskabady MH. 2017. The effect of Zataria multiflora on Th1/Th2 and Th17/Tregulatory in a mouse model of allergic asthma. Submitted to Forntiers in Pharmacology.ic & Appl Sci.
Lim YY, Quah EPL. 2007. Antioxidant properties of different cultivars of Portulaca oleracea. Food Chemistry, 103: 734-740.
Luksza AR, Jones DK. 1982. Comparison of whole-blood eosinophil counts in extrinsic asthmatics with acute and chronic asthma. British Med J, 285: 1229–1231.
Malek F, Boskabady MH, Borushaki MT, Tohidi M. 2004. Bronchodilatory Effect of Portulaca oleracea in airways of asthmatic patients. J Ethnopharmacol, 93: 57-62.
Neamati ABoskabady MHAfshari JTHazrati SMRohani AH. 2009. The effect of natural adjuvants on tracheal responsiveness and cell count in lung lavage of sensitized guinea pigs.Respirology, 14: 877-884.
Okwuasaba F, Ejike C, Parry O. 1986. Skeletal muscle relaxant properties of the aqueous extract of Portulaca oleracea. J Ethnopharmacol, 17: 139-160.
Oluwol FS, Oyediji KO. 2007. Calcium ions influences smooth muscle relaxant response to aqueous extract of Portulaca oleracea. J Med Sci, 7: 238-242.
Parry O, Okwuasaba F, Ejike C. 1987. Skeletal muscle relaxant action of an aqueous extract of Portulaca oleracea in the rat. J Ethnopharmacol, 19: 247- 253.
Parry OOkwuasaba FEjike C. 1988. Effect of an aqueous extract of Portulaca oleracea leaves on smooth muscle and rat blood pressure. J Ethnopharmacol, 22: 33-44.
Qureshi S, Memon SA, Laghari AJ, et al. 2009. Metabolic total protein investigation in local industry workers exposed to acid anhydride. Sindh Univ Res J, 41: 63-66.
Rao J, Jayasree T, Mallikarjuna Rao B, Kumar S and Kumar V. 2012. Evaluation of the Anti-nociceptive and Anti-inflammatory Activities of the Pet: Ether Extract of PortulacaOleracea (Linn.). J Clin Diagn Res, 6: 226-230.
Rasheed AN, Afifi FU, Disi AM. 2003. Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI- 1. J Ethnopharmacol, 88: 131-136.
Rasheed AN, Afifi FU, Shaedah M, Taha MO. 2004. Investigation of the active constituents of Portulaca oleraceae L. (Portulacaceae) growing in Jordan. Pak J Pharm Sci, 17: 37-45.
Salama AA, Zaki HF, El-Shenawy SM, EL-Denshary, ESM, Ismaiel I. 2012. Effects of Fish oil and Dexamethasone in Experimentally-Induced Bronchial Asthma. Aust J Basic Appl Sci, 6: 497-506.
Salmon M, Walsh D, Huang T, Barnes P, Leonard T, Hay D, Chung K. 1999. Involvement of cysteinyl leukotrienes in airway smooth muscle cell DNA synthesis after repeated allergen exposure in sensitized Brown Norway rats. Br J Pharmacol, 127: 1151-1158.
Shakeri F, Boskabady MH. 2017. Anti-inflammatory, antioxidant and immunomodulatory effects of curcumin in ovalbumin-sensitized rat. BioFactor Epub :1-10.
Shakeri F, Soukhtanloo M, Boskabady MH. 2017. The effect of hydro-ethanolic extract of Curcuma longa rhizome and curcumin on total and differential WBC and serum oxidant, antioxidant biomarkers in rat model of asthma. Iran J Basic Med Sci, 20: 155-165.
Tang XY, Yu HP, Deng HJ, Chen X, Fan HZ, Gong YX, et al. 2011. Pathogenic mechanism of CD8(+) CD28(-) T cell and the effect of dexamethasone in asthmatic mouse. Zhonghua Yi Xue Za Zhi, 91: 1861-1865.
Uddin MK, Juraimi AS, Ali ME, Ismail MR. 2012. Evaluation of antioxidant properties and mineral composition of purslane (Portulaca oleracea L.) at different growth stages. Int J Mol Sci, 13: 10257-10267.
Vadas P, Pruzanski W. 1986. Role of secretory Phospholipase A2 in the pathology of disease. Lab Invest, 55: 391-404.
Xio FY, Lu FE, Xu J. 2004. Mechanism of different parts of Portulaca oleracea in ameliorating lipid metabolic disorder in type 2 diabetic rats. Chine J Clin Rehabil, 8: 5042-5044.
Yang Z, Liu C, Xiang L, Zheng Y. 2009. Phenolic alkaloids as a new class of antioxidants in Portulaca oleracea. Phytother Res, 23: 1032–1035.
Zakaria MNM, Islam MW, Radhakrishnan R, Habibullah M, Chan K. 1998. Evaluation of anti-inflammatory activity of Portulaca species. J Pharm Pharmacol, 50: 227-231.