Antiplasmodial activity and cytotoxicity of ethanol extract of Zea mays root

Document Type : Original Research Article


1 Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria

2 Department of Chemistry, University of Uyo, Uyo, Nigeria

3 Department of Zoology, University of Abuja, Abuja, Nigeria

4 Department of Clinical Pharmacy and Biopharmacy, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria


Objective:Zea mays root decoction that has been traditionally used for the treatment of malaria by various tribes in Nigeria, was evaluated for antimalarial potential against malaria parasites using in vivo and in vitro models.
Materials and Methods: The root extract of Zea mays was investigated for antimalarial activity against Plasmodium berghei in mice using rodent malaria models; suppressive, prophylactic and curative tests and in vitro antiplasmodial activity against chloroquine-sensitive (Pf 3D7) and resistant (Pf INDO) strains of Plasmodium falciparum using SYBR green assay method. Median lethal dose and cytotoxic activity against HeLa and HEKS cells were assessed and phytochemical screening was also carried out using standard procedures.
Results: The LD50 value of root extract was found to be 474.34 mg/kg. The crude extract (45-135 mg/kg, p.o) showed significant (p<0.05-0.001) antimalarial activity against P. berghei infection in suppressive, prophylactic and curative tests with a prolonged survival time. The crude extract also showed moderate activity against both chloroquine-sensitive (Pf 3D7) and resistant (Pf INDO) strains of P. falciparum with an IC50 value of 71.62±3.38 μg/ml (for Pf 3D7) and 63.76±4.12 μg/ml (for Pf INDO). The crude extract was not cytotoxic to the two cell lines tested with TC50 of >100 μg/ml against both HeLa and HEKS cell lines.
Conclusion: These results suggest that the root extract of Zea mays possesses antimalarial activity against both chloroquine-sensitive and resistant malaria and these data justify its use in ethnomedicine to treat malaria infections.


Main Subjects

Abo KA, Fred-Jaiyesimi AA, Jaiyesimi AEA. 2008. Ethnobotanical studies of medicinal plants used in the management of diabetes mellitus in South Western Nigeria. J Ethnopharmacol, IS: 67-71.
Al-Adhroey AH, Nor ZM,Al-Mekhlafi HM, Amran AA, Mahmud R. 2011. Antimalarial activity of methanolic leaf extract of Piper betle L. Molecules, 16:107-118.
Attioua B, Lagnikab L, Yeoc  D, Antheaumed  C, Kaisere M, Wenigerf B, Lobsteinf A, Vonthron-Sénécheauf C. 2011. In vitro antiplasmodialand antileishmanialactivities of flavonoids from Anogeissus leiocarpus (combretaceae). Int J Pharmaceut Rev Res, 11:1-6.
Balasubramanian K, Jincy PA, Padma PR. 2015. Influence of methanolic extract of Zea mays leaves against CCL4 and H2O2 induced oxidative stress in Drosophila melanogaster.Indo Amer J Pharm Res, 5: 566-577.
Balasubramanian K, Padma PR. 2013. Anticancer activity of Zea mays leaf extracts on oxidative stress-induced Hep2 Cells. J Acupunct MeridStud, 6:149-158.
Balasubramanian K, Padma PR. 2012. Screening of Antioxidant Properties of Zea mays Leaves at different time periods of growth. Indo Amer J Pharm Res,5:4034-4037.
Balasubramanian K, Vidhya A, Thiruselvi M, Sudhadevi   M, Padma PR. 2014. Zea mays leaf extracts exhibits anticancer property and enhance the chemotherapeutic action of etoposide in cancer cells. Indo AmerJ Pharm Res, 4: 1530-1539.
Christensen SB, Kharazmi A. 2001. Antimalarial natural products.  Isolation, characterization and biological properties.  In. Bioactive compounds from natural sources: Isolation, characterization and biological properties. Ed. Tringali, C. London, Taylor & Francis.  Pp.379- 432.
Cimanga RK, Tona GL, Kambu OK, Mesia  GK, Muyembe JJ, Apers S, Totte J, Pieters, L, Vlietinck AJ. 2009. Antimalarial, antiamoebic and cytotoxic activities of some extracts and isolated constituents from the leaves of Morinda morindoides (Baker) Milne-Redh. (Rubiaceae). Recent Prog Med Plants, 25:225–242.
Dong J, Cai L, Zhu X, Huang X, Yin T, Fang H, Ding Z. 2014. Antioxidant activities and phenolic compounds of cornhusk, corncob and Stigma maydis. J Braz Chem Soc, 25: 1956-1964.
Ferreira JF, Luthria DL, Sasaki T, Heyerick A. 2010. Flavonoids from Artemisia annua L. as antioxidants and their potential synergism with artemisinin against malaria and cancer. Molecules, 15:3135-3170.
Foster S, Duke JA.1990. Field Guide 10 Medical Plants: Eastern and Central North America. Houghton MifAin, Boston.
Ganesh D, Fuehrer H,  Starzengrüber P, Swoboda P, Khan WA,  Reismann J A, Mueller MS, Chiba P, Noedl H. 2012. Antiplasmodial activity of flavonol quercetin and its analogues in Plasmodium falciparum: evidence from clinical isolates in Bangladesh and standardized parasite clones. Parasitol Res, 110: 2289-2295.
Gill LS. 1992. Ethnomedical Uses of Plants in Nigeria. Uniben Press, Benin, Nigeria, p. 249.
Hatzakis E, Opesenica I, Solaja BA, Stratakis M. 2007. Synthesis of novel polar derivatives of the antimalarial endoperoxides. ARKIVOC, VIII: 124-125.
Homburger F. 1989.  In vivo testing in the study of toxicity and safety evaluation.  In: A guide to general toxicology.  Marquis J. K .  (Ed).  2nd Edn Karger, New York.
Horgen FD, Madulld DA, Angerhofer CK, Pezzuto JM, Soejarto DD, Farnsworth NR.1997.Isolation of gallic acid esters as antiplasmodial constituents of Swintonia foxworthyi (Anacardiaceae). Phytomed, 4: 353- 356.
Kirmizibekmez H, Calis I, Perozzo R, Brun R, Donmez AA, Linden A. 2004. Inhibiting activities of the secondary metabolites of Phlomis brunneogaleata against parasitic protozoa and plasmodial enoyl-ACP reductase, a crucial enzyme in fatty acid biosynthesis. Planta Med, 70:711.
Kirby GC. 1997. Plants as a source of antimalarial drugs. Trop  Doct, 27(Suppl 1):7–11.
Kirby GC, O’Neill MJ, Phillipson JD,  Warhurst DC. 1989. In vitro studies on the mode of action of quassinoids with against chloroquine resistant Plasmodium falciparum. Biochem Pharmacol, 38: 4367-4374.
 Knight DJ, Peters W. 1980. The antimalarial action of N- Benzyloxydihydrotriazines and the studies on its mode of action. Ann Trop Med Parasitol, 74: 393-404.
Liao YF, Kupchan SM, Horwitz SB. 1976.Mode of action of antitumour compound bruceantin, an inhibitor of protein synthesis. Mol Pharmacol, 12:167 – 176.
Lorke D.1983. A new approach to practical acute toxicity testing. Arch Toxicol, 54: 275-286.
 Lui KC, Yang SC, Roberts MF. 1992. Antimalarial activity of Artemisia annua flavonoids from whole plants and cell cultures.  Plants Cell, II: 637-640.
Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 65:55–63.
Odetola A, Basir O. 1980. Evaluation of Antimalarial properties of some Nigerian Medicinal Plants. In: Sofowora A, Editor. Proceeding of African Bioscience Network, Federal Ministry of Science and Technology, Nigerian Society of Pharmacology and Drug Research and Production unit, University of Ife organized Workshop, Ife. pp. 275- 283.
Osagie AU, Eka OU. 1998. Nutritional Quality of Plant Foods.Post Harvest Research Unit, University of Benin, Benin, Nigeria, pp 34-41.
Owoyele BV, Negedu MN, Olaniran SO,  Onasanwo SA, Oguntoye SO, Sanya JO, Oyeleke SA, Ibidapo AJ, Soladoyel AO. 2010. Analgesic and anti-inflammatory effects of aqueous extract of Zea mays husk in male wistar rats. J Med Food,13:343-347.
Park S, Takano Y, Matsuura H, Yoshihara T. 2004. Antifungal compounds from the root and root exudate of Zea mays. Biosci Biotech Biochem, 68: 1366-1368,
Peters W. 1965. Drug Resistance in Plasmodium berghei. Exp Parasitol, 17: 80-89.
Philipson JD, Wright CW.1991. Antiprotozoal compounds from plants sources.  Planta Med, 57: 553-559.
Ryley JF, Peters W. 1970. The antimalarial activity of some quinolone esters. Ann Trop Med Parasitol, 84: 209-222.
Simmonds N W. 1979. Evolution of Crop Plants. Longman. London.  pp. 128-129.
Smilkstein M, Sriwilaijaroen N, Kelly J X, Wilairat P, Riscoe M. 2004. Simple and inexpensive fluorescence-based technique for high throughput antimalarial drug screening. Antimicrob Agents Chemother,.  48: 1803–1806.
Sofowora  A. 1993. Medicinal Plants and Traditional Medicine in Africa. 2nd ed. Spectrum Books Limited, Ibadan, Nigeria.  
Teffo LS, Aderogba MA, Eloff JN. 2010. Antibacterial and antioxidant activities of four Kaempferol methyl ethers isolated from Dodonaea viscosa Jacq. Var. angustifolia leaf extracts. South Afr J Bot, 76:25–29.
Trager W, Jensen J B. 1976. Human malaria parasites in continuous culture. Science,193: 673–675.
Trease GE, Evans WC. 1989. Pharmacognosy. 13th edn. Bailliere Tindall, London.  Pp. 683-684.