Antinociceptive action of Vanillosmopsis arborea in male mice

Document Type : Original Research Article


1 Department of Biology at the Federal University of Cariri, Brejo Santo – CE, Brazil

2 Department of Pharmacology, University of Santa Maria, Santa Maria- RS, Brazil

3 Department of Chemical Biological at the Regional University of Cariri, Crato – CE, Brazil

4 Department of Fharmacology at the University of Fortaleza, Fortaleza – CE, Brazil


Objective: Vanillosmopsis arborea Baker (Asteraceae) has high economic value from Chapada to Araripe and its bark essential oil is a potential source of alpha-bisabolol. The present study aimed to elucidate the antinociceptive and antipruritic properties of the essential oil of V. arborea Baker (EOVA) in mice.
Materials and Methods: The antinociceptive activity was assessed using the capsaicin, glutamate, hot plate and cold allodynia tests. The antipuritic effects were also verified based on histamine-induced scratching behavior.
Results: EOVA reduced the paw licking induced by capsaicin, but not that induced by glutamate. The essential oil increased the latency time in the hot plate, attenuated the cold allodynia induced by acetone and inhibited histamine-induced scratching behavior.
Conclusion: The experimental data demonstrated that EOVA showed central and peripheral antinociceptive activity and antipruritic effect.


Main Subjects

Baron R, Binder A, Wasner G. 2010. Neuropathic pain: diagnosis, pathophysiological mechanisms, and treatment. Lancet Neurol, 9: 807–819.
Beirith A, Santos RSA and Calixto JB, 2002. Mechanisms underlying the nociception and paw oedema caused by injection of glutamate into the mouse paw. Brain Res, 924.2: 219-228.
Benson HAE 2005. Transdermal drug delivery: Penetration enhancement techniques. Curr Drug Deliv; 2 : 23 – 33.
Choi AL, Sun G, Zhang Y, Grandjean P. 2012. Developmental fluoride neurotoxicity: a systematic review and meta-analysis. Environ Health Perspect, 120:1362–1368.
Coderre TJ, 1993. The role of excitatory amino acid receptors and intracellular messengers in persistent nociception after tissue injury in rats. Mol Neurobiol, 7: 229–246.
Cunha TM, Verri WAJ, Schivo IR, Napimoga MH, Parada CA, Poole S, Teixeira MM, Ferreira SH, Cunha FQ. 2008. Crucial role of neutrophils in the development of mechanical inflammatory hypernociception. J Leukoc Biol, 83: 824-832.
Dickenson. 1997. Mechanisms of central hypersensitivity: excitatory amino acid mechanisms and their control, in: A. Dickenson, J.-M. Besson (Eds.), The Pharmacology of Pain, Springer, Berlin, 167–209.
Dogrul A, Ossipov MH, Porreca F. 2009. Differential mediation of descending pain facilitation and inhibition by spinal 5-HT3 and 5-HT7 receptors. Brain Res, 1280: 52-59.
Eddy, NB and Leimbach D. 1953. Synthetic analgesics. II. Dithienylbutenyl-and dithienylbutylamines. J Pharmacol Exp Therap, 107: 385-393.
Gadotti VM, Santos AR, Silva CM, Schmeling LO, Machado C and Liz FH. 2005. Antinociceptive action of the extract and the flavonoid quercitrin isolated from Bauhinia microstachya leaves. J Pharmacol, 57: 1345-1351.
Hudspith MJ. 1997. Glutamate: a role in normal brain function, anaesthesia, analgesia and CNS injury. Br J Anaesth, 78: 731– 747.
International Association for the Study of Pain. Pain, IASP Pain Terminology. 1994.
Leite GO, Sampaio RS, Leite LHI, Menezes IRA, Costa JGM, Campos AR. 2011. Attenuation of visceral pain in mice by the essential oil from Vanillosmopsis arborea Baker. Rev Dor São Paulo, 12: 46-9.
Leite LHI, Leite GO, Coutinho TS, Sousa SDG, Sampaio RS, Costa JGM, Menezes IRA, Campos AR. 2014. Topical Antinociceptive Effect of Vanillosmopsis arborea Baker on Acute Corneal Pain in Mice. Evid Based Compl  Alter Med, Article ID 7086364.
Lipton SA, Rosenberg PA. 1994. Excitatory amino acids as a final common pathway for neurologic disorders. N Engl J Med, 330: 613–622.
Petersen KL, Rowbotham MC. 1999. A new human experimental pain model: the heat/capsaicin sensitization model. Neuroreport, 10: 1511–1516.
Rang HP, Dale MM, Ritter JM, Moore PK. 2004. Distúrbios neurodegenerativos. In: Farmacologia. 7.Ed., Rio de Janeiro: Elsevier Editora Ltda, 559-573.
Santos NKA, Campos AR, Costa JGM. 2015. The essential oil from Vanillosmopsis arborea Baker (Asteraceae) presents antinociceptive, anti-inflammatory, and sedative effects. Int J Green Pharm, 9:138-142.
Simone DA, Sorkin LS, Oh U, Chung JM, Owens C. 1991. Neurogenic hyperalgesia: central neural correlates in responses of spinothalamic tract neurons. J Neurophysiol, 66: 228–246.
Teixeira MJ.2004. Mecanismos de ocorrência de dor. Revista de Medicina 83: 69-113.
Westlund KN. 2006. The dorsal horn and hyperalgesia. In: Cervero F, Jensen TS, editors. Handbook of Clinical Neurology 2006, Vol 81 (3rd series): Pain. Amsterdam: Elsevier B.V. 178–186.
Wokovich AM, Prodduturi S, Doub WH, Hussain AS, Buhse LF. 2006. Transdermal drug delivery system (TDDS) adhesion as a critical safety, efficacy and quality attribute. Eur J Pharm Biopharm, 64:1-8.
WHO Normative Guidelines on Pain Management. World Health Organization, Geneva.