Evaluation of wound healing, antioxidant and antimicrobial efficacy of Jasminum auriculatum Vahl. leaves

Document Type : Original Research Article


1 Department of Pharmacognosy and Phytochemistry. Hindu College of Pharmacy, Sonipat, Haryana-131001, India

2 Department of Pharmaceutical Sciences. Birla Institute of Technology,Mesra, Jharkhand-835215, India


Objective: To validate the ethno-therapeutic claim of the traditionally used plant Jasminum auriculatum (J. auriculatum) in skin diseases, by evaluating its wound healing potential along with its antioxidant and antimicrobial properties; so as to understand their role in wound healing.
Materials and Methods: Excision and incision wound models were used to evaluate the wound healing activity on albino rats. The wound healing potential was assessed by measuring rate of wound contraction, epithelialization period, hydroxyproline content, skin breaking strength and histopathological  parameters. Reference standard drug was Nitrofurazone ointment. The antioxidant activity was determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method. The antimicrobial activity was determined by agar well diffusion method and minimum inhibitory concentration by serial dilution method.
Results: Higher rate of wound contraction (83.66±0.50% on 15th day), decrease in the period of epithelialization (17.83±1.6days), higher skin breaking strength (170.71±1.52g), higher collagen content and favourable histopathological changes revealed that topical application of ointment containing successive ethanolic extract (S.E.E) of J. auriculatum leaves has the most potent wound healing ability compared to control group in both  the models studied.  The DPPH radical scavenging activity of successive ethanolic extract was found to be 33.39µg/ml. Successive ethanolic extract was found to be most effective against Pseudomonas auregenosa having a zone of inhibition 16.65±0.6mm and the minimum inhibitory concentration was 0.78mg/ml.
Conclusion: The data of this study indicate that successive ethanolic extract of the leaves exhibit potent wound healing, antioxidant and antimicrobial properties. This justifies the ethno-medicinal use of plant for the treatment of wound and microbial infections.


Main Subjects

Abd-Elsalam KA, Aly NI, Abdel-Satar AM, Khalil SM, Verreet AJ.2013. PCR identification of Fusarium genus based on nuclear ribosomal-DNA sequence data. African J Biotech, 2: 82-85.
Adly AAM. 2010. Oxidative stress and disease: an updated review.  Res J Immunol, 3: 129-145. 
Allen LV, Popvich NG, Ansel HC.2008. Ansel’s Pharmaceutical Dosage forms and Drug Delivery Systems. 8th ed., p. 276 – 297, New Delhi: Wolter Kluwer (India) Pvt. Ltd.
Anna GG, Marlena DM, Irena M. 2012. DPPH radical scavenging activity and Phenolic compound content in different leaf extracts from selected Blackberry species. Acta Biologica Cracoviensia Series Botanica, 54: 32–38.
Bairy KL, Rao CM. 2001.Wound healing profiles of Ginkgo biloba. J Nat Remedies, 1: 25-27.
Cappucino JG, Sherman N. 1996. Microbiology: A Laboratory Manual, 4th ed., p. 263-64, England: Addison-Wiley Longman. 
Deshpande PJ, Pathak SN. 1965 (b). Wound healing under the influence of Jasminum auriculatum- a study of tensile strength. Surg. J Delhi, 1: 273.
Deshpande PJ, Pathak SN. 1966 (a). Tensile strength study in skin and musculoperitoneal wound under the influence of Jasminum auriculatum in rats. Med Surg, 6: 21.
Deshpande PJ, Pathak SN.1966 (b). Comparative study of healing in experimental burns under the influence of ghee and Jasminum medicated ghee used topically. J Res Indian Med, 1: 81.
Deshpande PJ, Shankaran PS, Pathak SN. 1965 (a). The role of Jasminum auriculatum in experimental wound healing. Med Surg, 5: 27.
Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. 2006. Antioxidant activity of some algerian medicinal plants extracts containing phenolic compound. Food Chem, 97: 654–660.
Ehrlich HP, Hunt TK. 1969. Effect of cortisone and anabolic steroids on tensile strength of a healing wound.  Ann Surg., 170: 203-206.
Essawi T, Srour M. 2000. Screening of some Palestinian medicinal plants for antibacterial activity. J Ethnopharmacol, 70: 343-349.
Fulzele SV, Sattuwar PM, Joshi SB, Dorle AK. 2002. Wound healing activity of Hingvadya Ghrita in rats. Indian Drugs, 39: 606-609.
Getie M, Gebre MT, Mariam T, Reitz R, Neubert RH. 2002. Evaluation of the release profile of flavonoids from topical formulations of the crude extract of the leaves of Dodonea viscosa (Sapindaceae). Pharmazie, 57: 320-322.
Gurung S, Basnet NS. 2009. Wound healing properties of Carica papaya latex: in -vivo evaluation in mice burn model. J Ethnopharmacol, 121: 338-341.
Jayasutha J, Monic J, Nithila S. 2011. Evaluation of wound healing activity of ethanolic extract of Aristolochia bracteata and Cassia tora on wistar albino rats. Int J Pharm Tech Res, 3: 1547-50.
Lee KH. 1968. Studies on the mechanism of action of salicylates II. Effects of vitamin A on wound healing retardation action of aspirin.  J Pharm Sci, 57: 1238-1240.
Mertz P, Ovington L. 1993. Wound healing microbiology. Dermatologic Clinics, 11: 739-747.
Mittal A, Sardana S, Pandey A. 2013. Herbal boon for wounds. Int J Pharm Pharma Sci, 5: 1-12.
Morton JJP, Malone MH. 1972. Evaluation of vulnerary activity by an open wound procedure in rats. Arch Int Pharmacodyn Therap, 196: 117-126.
Murti K, Kumar U. 2012. Enhancement of wound healing with roots of Ficus racemosa L. in albino rats. Asian Pac J Trop Biomed, 276-80.
Muthusamy SK, Kirubanandan S, Sripriya, Sehgal PK. 2008. Triphala promotes healing of infected full-thickness dermal wound. J Surg Res, 144: 94.
Nalwaya N, Pokharna G, Deb L, Jain NK. 2009. Wound healing activity of latex of Calotropis gigantea. Int J Pharm Pharma Sci, 1: 176.
Nayak SB, Lexley M, Pereira P. 2006. Catharanthus roseus flower extract has wound healing activity in sprague dawley rats. BMC Complement Alter Med, 6: 41-46.
Neuman RE, Logan MA. 1950. The determination of collagen and elastin in tissues. J Biol chem, 186: 549-556.
OECD guidelines for testing of chemicals. 2004. Acute Dermal Toxicity- 434, 1-13.
Scortichini M, Pia RM. 1991. Preliminary in vitro evaluation of the antimicrobial activity of terpenes and terpenoids towards Erwinia amylovora (Burrill). J Appl Bacteriol, 71: 109-112.
Shenoy RR, Sudheendra AT, Nayak PG, Paul P. 2011. Normal and delayed wound healing is improved by sesamol, an active constituent of Sesamum indicum Linn. in albino rats.  J Ethnopharmacol, 133: 608-12.
Shirwaikar A, Shenoy R, Udupa AL, Udupa SL, Shetty S. 2003. Wound healing property of ethanolic extract of leaves of Hyptis suaveolens with supportive role of antioxidant enzymes. Indian J Exp Biol, 41: 238-241.  
Sofowora EA. 1982. Medicinal plants and Traditional medicine in Africa, p. 256, Chichester: Wiley.
Souba WW, Wilmore D. 1999. Diet and nutrition in case of the patient with surgery, truma and sepsis. In: Shils M, Oslon J, Shike M, Ross AC, editor. Modern nutrition in health and disease. 9th ed., p. 1589-1618, Baltimore: Williams and Wilkins.
Suruse P, Kale MK, Gunde M, Amnerkar N, Pathak AK. 2011. Evaluation of wound healing activity of Arisaema leschenaultii (Blume). in rats. Pharmacia Lettre, 3: 200-6.
Trease GE, Evans WC. 1987. A text book of Pharmacognosy, p. 1055, Oxford: ELBS Baillere Tindal.
Tsuchiya H, Sato M, Miyazaki T, Fujiwara S, Tanigaki S, Ohyama M. 1996. Comparative study on the antibacterial activity of phyto-chemical flavones against methicillin-resistant S. aureus. J Ethnopharmacol, 50: 27-34.
Vats M, Singh H, Sardana S. 2011. Phytochemical screening and antimicrobial activity of roots of Murraya koenigii (Linn.) Spreng. (Rutaceae). Brazilian J Microbiol, 42: 1569-1573.
Werner S, Breeden M, Hubner G, Greenhalgh DG, Longaker MT. 1994. Induction of keratinocyte growth factor expression is reduced and delayed during wound healing in the genetically diabetic mouse. J. Investigation Dermatol. 103: 469-473.
Yaduvanshi B, Mathur R, Mathur SR, Velpandian T. 2011. Evaluation of wound healing potential of topical formulation of leaf juice of Tridax procmbens L. in mice. Indian J Pharm Sci, 73: 303-306.