The effects of different fractions of Coriandrum sativum on pentylenetetrazole-induced seizures and brain tissues oxidative damage in rats

Document Type : Original Research Article


1 Department of Physiology, School of Medicine, Jiroft university of medical Sciences, Jiroft, Iran

2 Neurogenic Inflammation Research Center & Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

3 Student Research Committee, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

4 Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

5 Neurocognitive Research Center & Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


Objective: In the present work, the effects of different fractions of Coriandrum sativum (C. sativum), on pentylenetetrazole (PTZ)-induced seizures and brain tissues oxidative damage were investigated in rats.
Materials and Methods: The rats were divided into the following groups: (1) vehicle, (2) PTZ (90 mg/kg), (3) water fraction (WF) of C. sativum (25 and 100 mg/kg), (4) n-butanol fraction (NBF) of C. sativum (25 and 100 mg/kg), and (5) ethyl acetate fraction (EAF) of C. sativum (25 and 100 mg/kg).
Results: The first generalized tonic-clonic seizures (GTCS) latency in groups treated with 100 mg /kg of WF or EAF was significantly higher than that of PTZ group (p< 0.01). In contrast to WF, the EAF and NBF were not effective in increasing the first minimal clonic seizure (MCS) latency. Malondialdehyde (MDA) levels in both cortical and hippocampal tissues of PTZ group were significantly higher than those of control animals (p< 0.001). Pretreatment with WF, NBF, or EAF resulted in a significant reduction in the MDA levels of hippocampi (pConclusion: The present study showed that different fractions of C. sativum possess antioxidant activity in the brain and WF and EAF of this plant have anticonvulsant effects.


Main Subjects

Aldarmaa J, Liu Z, Long J, Mo X, Ma J, Liu J. 2010. Anti-convulsant effect and mechanism of Astragalus mongholicus extract in vitro and in vivo: protection against oxidative damage and mitochondrial dysfunction. Neurochem Res, 35:33-41.
Costello DJ, Delanty N. 2004.Oxidative injury in epilepsy: potential for antioxidant therapy? Expert Rev Neurother, 4:541-53.
Coyle JT, Puttfarcken P. 1993. Oxidative stress, glutamate, and neurodegenerative disorders. Science, 262:689-95.
Dhanapakiam P, Joseph JM, Ramaswamy VK, Moorthi M, Kumar AS. 2008. The cholesterol lowering property of coriander seeds (Coriandrum sativum): mechanism of action. J Environ Biol, 29:53-6.
do Vale TG, Furtado EC, Santos JG Jr, Viana GS. 2002. Central effects of citral, myrcene and limonene, constituents of essential oil chemotype from Lippia Alba (Mill.) n.e. Brown. Phytomedicine, 9: 709-14.
Eastwood MA. 1999. Interaction of dietary antioxidants in vivo: how fruit and vegetables prevent disease? QJM, 92:527-30.
Ebrahimzadeh Bideskan AR, Hosseini M, Mohammadpour T, Karami R, Khodamoradi M, Nemati Karimooy H, Alavi H . 2011. Effects of soy extract on pentylenetetrazol-induced seizures in ovariectomized rats. Zhong Xi Yi Jie He Xue Bao, 9:611-8.
Eidi M, Eidi A, Saeidi A, Molanaei S, Sadeghipour A, Bahar M, Bahar K. 2009. Effect of coriander seed (Coriandrum sativum L.) ethanol extract on insulin release from pancreatic beta cells in streptozotocin-induced diabetic rats. Phytother Res, 23: 404-6.
Elisabetsky E, Marschner J, Souza DO . 1995. Effects of linalool on glutamatergic system in the rat cerebral cortex. Neurochem Res, 20: 461-465.
Emam Ghoreyshi M, Heydari Hamedani G. 2008. Effect of extract and essential oil of Coriandrum sativum seed against pentylenetetrazole-induced seizure. Pharm Sci, 3:1.
Engelborghs S, D'Hooge R, De Deyn PP. 2000. Pathophysiology of epilepsy. Acta Neurol. Belg, 100: 201-213
Frolich L, Riederer P. 1995. Free radical mechanisms in dementia of Alzheimer type and the potential for antioxidative treatment. Arzneimittel-Forschung, 45:443-6.
Ghorbani A, Rakhshandeh H, Asadpour E, Sadeghnia HR. 2011. Effects of Coriandrum sativum extracts on glucose/serum deprivation-induced neuronal cell death. Avicenna J Phytomed, 2:4-9.
Ghorbani A, Rakhshandeh H, Sadeghnia HR. 2013. Potentiating effects of Lactuca sativa on pentobarbital-induced sleep. Iran J Pharm Res, 12:401-6.
Ghorbani A, Youssofabad NJ, Rakhshandeh H. 2012. Effect of Viola tricolor on pentobarbital-induced sleep in mice. African J Pharm Pharmacol, 6:2503-9.
Ghorbani A, Sadeghnia HR, Asadpour E. 2015. Mechanism of protective effect of lettuce against glucose/serum deprivation-induced neurotoxicity. Nutr Neurosci, 18:103-9.
Golechha M, Bhatia J, Arya DS. 2010. Hydroalcoholic extract of Emblica officinalis Gaertn. Affords protection against PTZ-induced seizures, oxidative stress and cognitive impairment in rats. Indian J Exp Biol, 48:474-8.
Griebel G, Perrault G, Tan S, Schoemaker H, Sanger DJ. 1999. Pharmacological studies on synthetic flavonoids: comparison with diazepam. Neuropharmacology, 38:965-77.
Gupta YK, Briyal S. 2006. Protective effect of vineatrol against kainic acid induced seizures, oxidative stress and on the expression of heat shock proteins in rats. Eur Neuropsychopharmacol, 16:85-91.
Halliwell B. 1992. Oxygen radicals as key mediators in neurological disease: fact or fiction? Ann Neurol, 32:S10-5.
Hashim MS, Lincy S, Remya V, Teena M, Anila L. 2005. Effect of polyphenolic compounds from Coriandrum sativum on H2O2-induced oxidative stress in human lymphocytes. Food chem, 92:653-60.
Hosseini M, Ghasemzadeh Rahbardar M, Sadeghnia HR, Rakhshandeh H. 2011. Effects of different extracts of Rosa damascena on pentylenetetrazol-induced seizures in mice. Zhong Xi Yi Jie He Xue Bao, 9:1118-24.
Hosseini M, Harandizadeh F, Niazamand S, Soukhtanloo M, Mahmoudabady M. 2013. Antioxidant effect of Achillea wilhelmsii extract on pentylenetetrazole (seizure model)-induced oxidative brain damage in Wistar rats. Indian J Physiol Pharmacol, 57:418-24.
Hosseini M, Jafarianheris T, Seddighi N, Parvaneh M, Ghorbani A, Sadeghnia HR, Rakhshandeh H. 2012. Effects of different extracts of Eugenia caryophyllata on pentylenetetrazole-induced seizures in mice. Zhong Xi Yi Jie He Xue Bao, 10:1476-81.
Hosseini M, Pourganji M, Khodabandehloo F, Soukhtanloo M, Zabihi H. 2012. Protective effect of l-arginine against oxidative damage as a possible mechanism of its Bene. cial properties on spatial learning in ovariectomized rats. Basic Clin Neurosci, 3:36-44.
Hosseini M, Sadeghnia HR, Salehabadi S, Alavi H, Gorji A. 2009. The effect of L-arginine and L-NAME on pentylenetetrazole induced seizures in ovariectomized rats, an in vivo study. Seizure, 18:695-8.
Hosseini M, Sadeghnia HR, Salehabadi S, Soukhtanloo M. 2013. Contribution of estradiol in sex-dependent differences of pentylenetetrazole-induced seizures in rats. Acta Physiol Hung, 100:237-45.
Hosseinzadeh H, Madanifard M. 2000. Anticonvulsant effects of Coriandrum sativum L. seed extracts in mice. Arch Iran Med, 3:81-4.
Hosseinzadeh H, Sadeghnia HR. 2007.  Protective effect of safranal on pentylenetetrazol-induced seizures in the rat: involvement of GABAergic and opioids systems. Phytomedicine, 14:256-62.
Hosseinzadeh H, Sadeghnia HR. 2005.  Safranal, a constituent of Crocus sativus (saffron), attenuated cerebral ischemia induced oxidative damage in rat hippocampus. J Pharm Pharm Sci, 8:394-9.
Kang TH, Jeong SJ, Kim NY, Higuchi R, Kim Yc. 2000. Sedative activity of two flavonol glycosides isolated from the flowers of Albizzia julibrissin Durazz. J Ethnopharmacol, 71:321-3.
Kang TH, Jeong SJ, Kim NY, Higuchi R, Kim YC. 2000. Sedative activity of two flavonol glycosides isolated from the flowers of Albizzia julibrissin Durazz. J Ethnopharmacol, 71:321-3.
Khodabandehloo F, Hosseini M, Rajaei Z, Soukhtanloo M, Farrokhi E, Rezaeipour M. 2013. Brain tissue oxidative damage as a possible mechanism for the deleterious effect of a chronic high dose of estradiol on learning and memory in ovariectomized rats. Arq Neuropsiquiatr, 71:313-9.
Kudin AP, Kudina TA, Seyfried J, Vielhaber S, Beck H, Elger CE, Kunz WS. 2002. Seizure-dependent modulation of mitochondrial oxidative phosphorylation in rat hippocampus. Eur J Neurosci, 15:1105-14.
Kunzemann J, Herrmann K. 1977.  [Isolation and identification of flavon(ol)-O-glycosides in caraway (Carum carvi L.), fennel (Foeniculum vulgare Mill.), anise (Pimpinella anisum L.), and coriander (Coriandrum sativum L.), and of flavon-C-glycosides in anise. I. Phenolics of spices (author's transl)]. Z Lebensm Unters Forsch, 164:194-200.
Liang LP, Beaudoin ME, Fritz MJ, Fulton R, Patel M. 2007. Kainate-induced seizures, oxidative stress and neuronal loss in aging rats. Neuroscience, 147:1114-8.
Liu SH, Chang CD, Chen PH, Su JR, Chen CC, Chaung HC. 2012.  Docosahexaenoic acid and phosphatidylserine supplementations improve antioxidant activities and cognitive functions of the developing brain on pentylenetetrazol-induced seizure model. Brain Res, 1451:19-26.
Loscher W, Honack D, Fassbender CP, Nolting B. 1991. The role of technical, biological and pharmacological factors in the laboratory evaluation of anticonvulsant drugs. III. Pentylenetetrazole seizure models. Epilepsy Res, 8: 171-180.
Maldonado A, Ramos W, Perez J, Huaman LA, Gutierrez EL. 2010 .[Convulsive status epilepticus: clinico-epidemiologic characteristics and risk factors in Peru]. Neurologia, 25:478-84.
Meador KJ. 2002. Cognitive outcomes and predictive factors in epilepsy. Neurology, 58:S21-6.
Medina JH, Viola H, Wolfman C, Marder M, Wasowski C, Calvo D, Paladini AC. 1997. Overview--flavonoids: a new family of benzodiazepine receptor ligands. Neurochem Res, 22:419-25.
Mehla J, Reeta KH, Gupta P, Gupta YK. 2010. Protective effect of curcumin against seizures and cognitive impairment in a pentylenetetrazole-kindled epileptic rat model. Life Sci, 87:596-603.
Misharina TA, Samusenko AL. 2008.  [Antioxidant properties of essential oils from lemon, grapefruit, coriander, clove, and their mixtures]. Prikl Biokhim Mikrobiol, 44:482-6.
Misharina TA, Samusenko AL. 2008. Antioxidant properties of essential oils from lemon, grapefruit, coriander, clove, and their mixtures. Prikl Biokhim Mikrobil, 44:438-42.
Nassiri-Asl M, Mortazavi SR, Samiee-Rad F, Zangivand AA, Safdari F, Saroukhani S, Abbasi E. 2010. The effects of rutin on the development of pentylenetetrazole kindling and memory retrieval in rats. Epilepsy Behav, 18:50-3.
Nassiri-Asl M, Naserpour Farivar T, Abbasi E, Sadeghnia HR, Sheikhi M, Lotfizadeh M, Bazahang P. 2013.  Effects of rutin on oxidative stress in mice with kainic acid-induced seizure. J Integr Med, 11:337-42.
Oganesyan ET, Nersesyan ZM, Parkhomenko AY. 2007.  Chemical composition of the above-ground part of Coriandrum sativum. Pharm Chem J, 41: 149-53.
Paladini AC, Marder M, Viola H, Wolfman C, Wasowski C, Medina JH. 1999. Flavonoids and the central nervous system: from forgotten factors to potent anxiolytic compounds. J Pharm Pharmacol, 51:519-26.
Parfenova H, Leffler CW, Basuroy S, Liu J, Fedinec AL. 2012. Antioxidant roles of heme oxygenase, carbon monoxide, and bilirubin in cerebral circulation during seizures. J Cereb Blood Flow Metab, 32:1024-34.
Patel M. 2004. Mitochondrial dysfunction and oxidative stress: cause and consequence of epileptic seizures. Free Radic Biol Med, 37:1951-62.
Picq M, Cheav SL, Pringent AF. 1991.  Effect of two flavonoi compounds on central nervous system. Analgesic activity. Life Sci, 49: 1979-88.
Porter RJ, Cereghino JJ, Gladding GD, Hessie BJ, Kupferberg HJ, Scoville B, White, BG. 1984.  Antiepileptic drug development program. Cleve Clin Q, 51: 293-305.
Qureshi GA, Baig S, Sarwar M, Parvez SH. 2004. Neurotoxicity, oxidative stress and cerebrovascular disorders. Neurotoxicology, 25:121-38.
Rakhshandah H, Hosseini M. 2006. Potentiation of pentobarbital hypnosis by Rosa damascena in mice. Indian J Exp Biol, 44:910-2.
Rakhshandeh H, Sadeghnia HR, Ghorbani A. 2012.  Sleep-prolonging effect of Coriandrum sativum hydro-alcoholic extract in mice. Nat Prod Res.  Nat Prod Res, 26:2095-8.
Reilly C, Agnew R, Neville BG. 2011. Depression and anxiety in childhood epilepsy: a review. Seizure, 20:589-97.
Rodrigues AD, Scheffel TB, Scola G, Santos MT, Fank B, Freitas SCV, et al. 2012. Neuroprotective and anticonvulsant effects of organic and conventional purple grape juices on seizures in wistar rats induced by pentylenetetrazole. Neurochem Int, 60:799-805.
Sadeghnia HR, Kamkar M, Assadpour E, Boroushaki MT, Ghorbani A. 2013. Protective effect of safranal, a constituent of Crocus sativus, on quinolinic acid-induced oxidative damage in rat hippocampus. Iran J Basic Med Sci, 16:73-82.
Samojlik I, Lakic N, Mimica-Dukic N, Dakovic-Svajcer K, Bozin B. 2010. Antioxidant and hepatoprotective potential of essential oils of coriander (Coriandrum sativum L.) and caraway (Carum carvi L.) (Apiaceae). J Agric Food Chem, 58:8848-53.
Sander JW. 2003. The epidemiology of epilepsy revisited. Curr Opin Neurol, 16:165-70.
Satyanarayana S, Sushruta K, Sarma GS, Srinivas N, Subba Raju GV. 2004. Antioxidant activity of the aqueous extracts of spicy food additives--evaluation and comparison with ascorbic acid in in-vitro systems. J Herb Pharmacother, 4:1-10.
Sejima H, Ito M, Kishi K, Tsuda H, Shiraishi H. 1997. Regional excitatory and inhibitory amino acid concentration in pentylenetetrazole kindling and kindled rat brain. Brain Develop, 19: 171-175.
Sharma DR, Sunkaria A, Bal A, Bhutia YD, Vijayaraghavan R, Flora SJ, Gill KD. 2009. Neurobehavioral impairments, generation of oxidative stress and release of pro-apoptotic factors after chronic exposure to sulphur mustard in mouse brain. Toxicol Appl Pharmacol, 240:208-18.
Sharma M, Briyal S, Gupta YK. 2005. Effect of alpha lipoic acid, melatonin and trans resveratrol on intracerebroventricular streptozotocin induced spatial memory deficit in rats. Indian J Physiol Pharmacol, 49:395-402.
Sudha K, Rao AV, Rao A. 2001. Oxidative stress and antioxidants in epilepsy. Clin Chim Acta, 303:19-24.
Sugawara Y, Hara C, Tamura K, Fujii T, Nakamura K-I, Masujima T, Aoki T. 1998. Sedative effect on humans of inhalation of essential oil of linalool. Sensory evaluation and physiological measurement using optically active linalool. Anal Chim Acta, 365:293-9.
Sultana S, Ripa FA, Hamid K. 2010. Comparative antioxidant activity study of some commonly used spices in Bangladesh. Pak J Biol Sci, 13:340-3.
Ure JA, Perassolo M. 2000. Update on the pathophysiology of the epilepsies. J Neurol Sci, 177: 1-17.
White HS, Smith MD, Wilcox KS. 2007. Mechanisms of action of antiepileptic drugs. Int Rev Neurobiol. 81:85-110.
Xie T, Wang WP, Mao ZF, Qu ZZ, Luan SQ, Jia LJ, Kan MC. 2012. Effects of epigallocatechin-3-gallate on pentylenetetrazole-induced kindling, cognitive impairment and oxidative stress in rats. Neurosci Lett, 516:237-41.
Youdim KA, Shukitt-Hale B, Joseph JA. 2004. Flavonoids and the brain: interactions at the blood-brain barrier and their physiological effects on the central nervous system. Free Radic Biol Med, 37:1683-93.