Anti-proliferative and apoptotic effects of Ziziphus Jujube on cervical and breast cancer cells

Document Type : Short communication


1 Department of Pharmacology, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran

2 Department of Biology, Payam-e Noor University of Esfahan, Esfahan, Iran

3 Cellular & Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran

4 Department of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran


Objectives: Ziziphus Jujube (Jujube) plant has exhibited numerous medicinal and pharmacological properties including antioxidant and anti-inflammatory effects. This study was carried out to investigate its anti-cancer and pro-apoptotic abilities in human cervical and breast cancer cells in vitro.
Materials and Methods: The cervical OV2008 and breast MCF-7 cancer cells were incubated with different concentrations of Jujube aqueous extraction (0-3 mg/ml) for various times (0-72 h). Cell viability was assessed by Trypan Blue and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of two apoptosis-related genes in treated cells evaluated by quantitative Real Time -PCR analysis.
Results: Jujube significantly inhibited cancer cell viability in a dose- and time- dependent manner. Herb-induced apoptosis was associated with enhanced expression of Bax and decreased Bcl2 gene leading eventually to a time-dependent six fold increase in the Bax/Bcl-2 ratio.
Conclusions: These results indicated that Jujube may be a natural potential and promising agent to prevent or treat human cancers.


Main Subjects

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