Protective effect of Hibiscus sabdariffa against serum/glucose deprivation-induced PC12 cells injury

Document Type : Original Research Article


1 Neurocognitive Research center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

2 Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

3 Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


Objectives: Findings natural products with antioxidant and antiapoptotic properties has been one of the interesting challenges in the search for the treatment of neurodegenerative diseases including ischemic stroke. Serum/glucose deprivation (SGD) has been used as a model for the understanding of the molecular mechanisms of neuronal damage during ischemia in vitro and for the expansion of neuroprotective drugs against ischemia-induced brain injury. Recent studies showed that Hibiscus sabdariffa exert pharmacological actions such as potent antioxidant. Therefore, in this study we investigated the protective effect of extract of H. sabdariffa against SGD-induced PC12 cells injury.
Materials and Methods: Cells were pretreated with different concentrations of H. sabdariffa extract (HSE) for 2 hr, and then exposed to SGD condition for 6, 12 and 18 hr.
Results: SGD caused a major reduction in cell viability after 6, 12, and 18 hr as compared with control cells (p< 0.001). Pretreatment with HSE (30-500 𝜇g/mL) significantly increased cell viability following SGD insult for 6, 12 and 18 hr. A significant increase in cell apoptosis was seen in cells under SGD condition after 12hr as compared with control cells (p< 0.001). Pretreatment with HSE significantly decreased cell apoptosis subsequent SGD conditionafter12hr at concentration of 60, 125 and 250.
Conclusion:  These data showed that HSE had a protective property under SGD condition in PC12 cells, suggesting that H. sabdariffa has the potential to be used as a new therapeutic approach for neurodegenerative disorders.


Main Subjects

Afsharzadeh M, Tayarani-Najaran Z, Zare A, Mousavi SH. 2012. Protective Effect of Scutellarialitwinowii Extract on Serum/Glucose-Deprived Cultured PC12 Cells and Determining the Role of Reactive Oxygen Species. J Toxicol, 2012: 413279.
Ali BH, Al Wabel N, Blunden G. 2005. Phytochemical, pharmacological and toxicological laspects of Hibiscussabdariffa L.: a review. See comment in PubMed Commons below Phytother Res, 19: 369-375.
Amantea D, Marrone MC, Nistico R, Federici M, Bagetta G, Bernardi G, Mercuri NB. 2005. Oxidative stress in stroke pathophysiology validation of hydrogen peroxide metabolism as a pharmacological target to afford neuroprotection. Int Rev Neurobiol, 85: 363-374.
Chang YC, Huang HP, Hsu JD, Yang SF, Wang CJ. 2005. Hibiscus anthocyanins rich extract-induced apoptotic cell death in human promyelocytic leukemia cells. Toxicol Appl Pharmacol, 205: 201-212.
Chen CC, Chou FP, Ho YC, Lin WL, Wang C. P, Kao E. S, Huang AC, Wang CJ. 2004. Inhibitory effects of H. sabdariffa L. extract on low-density lipoprotein oxidation and anti-hyperlipidemia in fructose-fed and cholesterol-fed rats. See comment in PubMed Commons below J Sci Food Agric, 84, 1989–1996.
Chu LF, Wang WT, Ghanta VK, Lin CH, Chiang YY, Hsueh CM. 2008.Ischemic brain cell-derived conditioned medium protects astrocytes against ischemia through GDNF/ERK/NF-kB signaling pathway. Brain Res, 1239: 24-35.
Costa-Rocha I, Bonnlaender B, Sievers H, Pischel I, Heinrich M. 2014.Hibiscus sabdariffa L. - a phytochemical and pharmacological review. Food Chem, 165: 424-443.
Forouzanfar F, AfkhamiGoli A, Asadpour E, Ghorbani A, Sadeghnia HR. 2013. Protective Effect of Punicagranatum L. against Serum/Glucose Deprivation-Induced PC12 Cells Injury.Evid Based Complement Alternat Med, 2013: 716730.
Hillion JA, Takahashi K, Maric D, Ruetzler C, Barker JL, Hallenbeck JM. 2005. Development of an ischemic tolerance model in a PC12 cell line. J Cereb Blood Flow Metab, 25: 154-162.
khaghani SH, Razi F, MohammadianYajloo M, Paknejad M, Shariftabrizi A, Pasalar P. 2011. Selective Cytotoxicity and Apoptogenic Activity of Hibiscus Sabdariffa Aqueous Extract against MCF-7 Human Breast Cancer Cell Line.J Cancer Ther, 2: 394-400.
Lin HH, Chen JH, Kuo WH, Wang CJ. 2007. Chemopreventive properties of Hibiscus sabdariffa L. on human gastric carcinoma cells through apoptosis induction and JNK/p38 MAPK signaling activation. ChemBiol Interact, 165: 59-75.
Lin HH, Huang HP, Huang CC, Chen JH, Wang CJ. 2005. Hibiscus polyphenol-rich extract induces apoptosis in human gastric carcinoma cells via p53 phosphorylation and p38 MAPK/FasL cascade pathway. MolCarcinog, 43: 86-99.
Lorenz L, Dang J, Misiak M, TamehAbolfazl A, Beyer C, Kipp M. 2009. Combined 17beta-oestradiol and progesterone treatment prevents neuronal cell injury in cortical but not midbrain neurons or neuroblastoma cells. J Neuroendocrinol, 21: 841-849.
Moley K. H, MuecklerM. M. 2000. Glucose transport and apoptosis. Apoptosis, 5: 99–105.
Mousavi SH, Tayarani-Najaran Z, Asghari M, Sadeghnia HR. 2010. Protective effect of Nigella sativa extract and thymoquinone on serum/glucose deprivation-induced PC12 cells death. Cell MolNeurobiol, 30: 591-598.
Oboh G, Rocha J. B. T. 2008. Antioxidant and Neuroprotective Properties of Sour Tea (Hibiscus sabdariffa, calyx) and Green Tea (Camellia sinensis) on some Pro-oxidant-induced Lipid Peroxidation in Brain in vitro. Food Biophysics, 3: 382–389.
Reimann-Philipp U, Ovase R, Weigel PH, Grammas P. 2001. Mechanisms of cell death in primary cortical neurons and PC12 cells. J Neurosci Res, 64: 654-660.
Rosamond W, Flegal K, Friday G, Furie K, Go A, Greenlund K, Haase N, Ho M, Howard V, Kissela B, Kittner S, Lloyd-Jones D, McDermott M, Meigs J, Moy C, Nichol G, O'Donnell CJ, Roger V, Rumsfeld J, Sorlie P, Steinberger J, Thom T, Wasserthiel-Smoller S, Hong Y. 2007. Heart disease and stroke statistics--2007 update: a report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee. Circulation, 115: e69-171.
Sindi HA, Marshall LJ, Morgan MR. 2014.Comparative chemical and biochemical analysis of extracts of Hibiscus sabdariffa. Food Chem, 164: 23-29.
Tseng TH, Kao ES, Chu CY, Chou FP, Lin Wu HW, Wang CJ.1997.Protective effects of dried flower extracts of Hibiscus sabdariffa L. against oxidative stress in rat primary hepatocytes. Food ChemToxicol, 35: 1159-1164.
Woronowicz A, Amith SR, Davis VW, Jayanth P, De Vusser K, Laroy W, Contreras R, Meakin SO, Szewczuk MR. 2007.Trypanosome trans-sialidase mediates neuroprotection against oxidative stress, serum/glucose deprivation, and hypoxia-induced neurite retraction in Trk-expressing PC12 cells. Glycobiology, 17: 725-734.