Document Type : Short communication
Authors
1
Neuroscience Research Center, Basic Sciences Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
2
Metabolic Syndrome Research Center, Basic Sciences Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Medical Genetics Research Center, Basic Sciences Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
3
Department of Periodontics, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran
4
Department of Periodontics, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran; Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
5
Department of Pharmaceutical Sciences in Persian Medicine, School of Persian and Complementary Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6
Faculty of Medicine, Mashhad University of Medical Sciences, Khorasan Razavi, Iran
7
Medical Genetics Research Center, Basic Sciences Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract
Objective: To evaluate the in vitro antimicrobial activity of a toothpaste containing pomegranate (Punica granatum L.) peel 70% hydroalcoholicextract (PPE) against oral microorganisms and to assess its cytotoxicity in mammalian cells.
Materials and methods: Cytotoxicity was evaluated using the MTT assay on L929 fibroblasts after 24 hr of exposure to PPE toothpaste at concentrations ranging from 62.5 to 2000 µg/mL. Antimicrobial activity was assessed using the agar well diffusion method against Streptococcus mutans, Lactobacillus plantarum, and Candida albicans. Two dilutions of PPE toothpaste (1:1 and 1:3 v/v) were compared with a commercial toothpaste. Chlorhexidine (CHX) was used as the positive control, and distilled water served as the negative control.
Results: PPE toothpaste showed no significant cytotoxicity at any tested concentration and produced inhibition zones against all tested microorganisms. Although the commercial comparator produced the largest inhibition zones overall, the 1:1 PPE dilution showed comparable effects, particularly against C. albicans, and its performance was similar to that of CHX. The differences compared with CHX were not statistically significant.
Conclusion: The PPE toothpaste showed no significant cytotoxic effect on L929 fibroblasts (p > 0.05) and exhibited in vitro antimicrobial activity against common oral pathogens, supporting further evaluation in biofilm models and clinical studies.
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