Avicenna Journal of Phytomedicine

Avicenna Journal of Phytomedicine

Protective effects of Elaeagnus angustifolia L. fruit extract on CCl4-induced oxidative stress and inflammation in rats liver

Document Type : Original Research Article

Authors
masoud ojarudi 1
Ali Golchin 2
Hamid Reza Karamdel 1
Mohammad Valilo 1
Parviz Ranjbarvan 2
1 Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
2 Department of Applied Cell Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Islamic Republic of Iran
10.22038/ajp.2025.25915
Abstract
Objective: Hepatic cells face oxidative stress-induced damage, but plant antioxidants may offer protection. This study aimed to assess Elaeagnus angustifolia L. fruit extract's potential in shielding rat livers from CCl4 damage.
Materials and Methods: 30 Male Wistar rats were randomly divided into five groups: normal control (received distilled water), E. angustifolia hydroalcoholic extract control, CCl4 control, E. angustifolia extract pretreatment (600 mg/kg), and silymarin pretreatment (100 mg/kg). After 14 days of oral administration of extracts, CCl4 was injected intraperitoneally. The samples were collected 48 hr later. Histological and biochemical analyses were then carried out.
Results: CCl4 injection caused significant (p<0.001) changes in liver serum enzymes, lipid profile, bilirubin, total protein, serum albumin, antioxidant enzymes, malondialdehyde, Inflammatory cytokines, and liver tissue morphology. E. angustifolia extract pre-treatment significantly (p<0.05) returned changes to the normal state.
Conclusion: This study's findings revealed that E. angustifolia extract pretreatment could reduce liver injury caused by CCl4 in rats.
Keywords
Elaeagnus angustifolia L
Carbon tetrachloride
Liver damage
Oxidative stress
Antioxidant
Subjects
Avicenna Journal of Phytomedicine
Volume 15, Issue 5
September and October 2025
Pages 1519-1530