Protective effects of Elaeagnus angustifolia L. fruit extract on CCl4-induced oxidative stress and inflammation in rats liver

Ojarudi, Masoud; Golchin, Ali; Karamdel, Hamid Reza; Valilo, Mohammad; Ranjbarvan, Parviz

doi:10.22038/ajp.2025.25915

Protective effects of Elaeagnus angustifolia L. fruit extract on CCl4-induced oxidative stress and inflammation in rats liver

Document Type : Original Research Article

Authors

masoud ojarudi ¹

Ali Golchin ²

Hamid Reza Karamdel ¹

Mohammad Valilo ¹

Parviz Ranjbarvan ²

¹ Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

² Department of Applied Cell Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Islamic Republic of Iran

10.22038/ajp.2025.25915

Abstract

Objective: Hepatic cells face oxidative stress-induced damage, but plant antioxidants may offer protection. This study aimed to assess Elaeagnus angustifolia L. fruit extract's potential in shielding rat livers from CCl₄ damage.
Materials and Methods: 30 Male Wistar rats were randomly divided into five groups: normal control (received distilled water), E. angustifolia hydroalcoholic extract control, CCl₄ control, E. angustifolia extract pretreatment (600 mg/kg), and silymarin pretreatment (100 mg/kg). After 14 days of oral administration of extracts, CCl₄ was injected intraperitoneally. The samples were collected 48 hr later. Histological and biochemical analyses were then carried out.
Results: CCl₄ injection caused significant (p<0.001) changes in liver serum enzymes, lipid profile, bilirubin, total protein, serum albumin, antioxidant enzymes, malondialdehyde, Inflammatory cytokines, and liver tissue morphology. E. angustifolia extract pre-treatment significantly (p<0.05) returned changes to the normal state.
Conclusion: This study's findings revealed that E. angustifolia extract pretreatment could reduce liver injury caused by CCl₄ in rats.

Keywords

Elaeagnus angustifolia L

Carbon tetrachloride

Liver damage

Oxidative stress

Antioxidant

Subjects