Extracts of Apium graveolens (Celery) attenuate hepato-renal injury induced by chronic administration of gentamicin in mice through activation of Nrf2-antioxidant signaling pathways

Kouam, Arnaud Fondjo; Mokam, Mayelle Mepa; Ngounou, Eleonore; Kouoh, Ferdinand Elombo; Fifen, Rodrigue; Kongnyuy, Kerinyuy Juliene; Zeuko’o, Elisabeth Menkem; Nembo, Nembu Erastus; Djamen, Pascal Dieudonné Chuisseu; Njayou, Frédéric Nico; Moundipa, Paul Fewou; Asongalem, Emmanuel Acha

doi:10.22038/ajp.2024.25338

Extracts of Apium graveolens (Celery) attenuate hepato-renal injury induced by chronic administration of gentamicin in mice through activation of Nrf2-antioxidant signaling pathways

Articles in Press

Document Type : Original Research Article

Authors

¹ Medical Research and Applied Biochemistry Laboratory, Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, PO Box 63, Buea, Cameroon

² Pharmacology Laboratory, Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, PO Box 63, Buea, Cameroon

³ Anatomy Laboratory, Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, PO Box 63, Buea, Cameroon

⁴ Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty of Science, University of Yaoundé 1, P.O. Box 812, Yaounde 1, Cameroon

⁵ Laboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé 1, PO Box 812, Yaoundé, Cameroon

⁶ Higher Institute of Health Sciences, Université des Montagnes, P.O. Box 208, Bangangté, Cameroon

10.22038/ajp.2024.25338

Abstract

Objective: This study aimed at investigating the protective effect of extracts from Apium graveolens against gentamicin-induced hepato-renal toxicity.
Materials and Methods: The aqueous and hydro-ethanolic extracts of A. graveolens designated respectively as WAG and HAG were tested for their in vitro antioxidant activities. Then, their cytoprotective effects were assessed against gentamicin-induced cytotoxicity in primary mouse hepatocytes. Finally, mice were administered with gentamicin (20 mg/kg) and co-treated with HAG for 14 days, and histopathology, biochemical and molecular parameters related to gentamicin-induced toxicity were evaluated.
Results: HAG exhibited outstanding chemical antioxidant activities and preserved hepatocytes from gentamicin-induced cytotoxicity. HAG relieved liver and kidney histopathological and biochemical changes, and enhanced the mRNA level of Nrf2 and its target gene HO-1 in gentamicin-intoxicated mice.
Conclusion: HAG attenuates hepato-renal injuries induced by 14-days administration of gentamicin in mice through the activation of Nrf2-antioxidant signaling pathways.

Keywords