Document Type : Short communication
Authors
1
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences; Department of Traditional pharmacy, School of Persian Medicine, Kerman University of Medical Sciences, Kerman,Iran
2
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3
Yazd Neuroendocrine Research Center, Faculty of medicine, Shahid Sadoughi University of Medical Sciences and Health services, Yazd, Iran
4
Hematology and oncology research center, shahid Sadoughi University of medical Sciences, Yazd, Iran Children Growth Disorder Research Center, shahid Sadoughi University of Medical Sciences, Yazd, Iran
5
Department of Pharmacognosy, School of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
10.22038/ajp.2024.25298
Abstract
Objective: Ulcerative colitis is a chronic recurrent inflammatory bowel disease of unknown etiology. The anti-inflammatory, immunomodulatory, and antioxidant characteristics of Henna (Lawsonia inermis) fixed oil (HFO) imply that it may be advantageous for the treatment of colitis.
Materials and Methods: In this research, the effect of HFO in a Wistar albino rat model of acetic acid (AA)-induced ulcerative colitis, was examined. The animals received daily oral administration of either normal saline (10 ml/kg), HFO (100, 400, and 1600 µl/kg), or dexamethasone (2 mg/kg) for 5 days. A single intracolonic injection of 2 ml of a 4% (v/v) acetic acid solution was used to induce colitis. The levels of myeloperoxidase (MPO) and tumor necrosis factor-alpha (TNF-α) were measured.
Results: The administration of HFO at doses 400 and 1600 μl/kg showed a significant enhancement in the weight-to-length ratio of colon tissue in comparison to the control group. Furthermore, the increased amounts of HFO (400 and 1600 μl/kg) were associated with a significant reduction in ulcer severity, area, and index. However, examination of tissue samples revealed a decrease in the overall colitis index suggesting fewer inflammatory cells invaded the colonic regions of rats treated with HFO at doses of 400 and 1600 μl/kg. Moreover, the elevated MPO levels and TNF-α were significantly decreased following the administration of the fixed oil at these doses.
Conclusion: These findings indicate that HFO could potentially decrease the manifestations of experimental colitis in a dose-dependent manner.
Keywords
Main Subjects
)