The hydroalcoholic extract of Nasturtium officinale protectively inhibits apoptotic and inflammatory pathways in hepato- and nephrotoxicity: An in vivo study

Soudkhah, Sevil; Keyghobadi, Sahar; Shadboorestan, Amir; Gholami, Mahdi; Omidi Sarajar, Behnam; Salek Maghsoudi, Armin; Omidi, Mahmoud; Mohammadi Motamed, Saeed; Akbarzadeh Kolahi, Saeid; Rastegar-Pouyani, Nima; Hassani, Shokoufeh

doi:10.22038/ajp.2024.25213

The hydroalcoholic extract of Nasturtium officinale protectively inhibits apoptotic and inflammatory pathways in hepato- and nephrotoxicity: An in vivo study

Document Type : Original Research Article

Authors

Sevil Soudkhah ¹

Sahar Keyghobadi ¹

Amir Shadboorestan ²

Mahdi Gholami ³

Behnam Omidi Sarajar ⁴

Armin Salek Maghsoudi ³

Mahmoud Omidi ⁵

Saeed Mohammadi Motamed ⁶

Saeid Akbarzadeh Kolahi ¹

Nima Rastegar-Pouyani ³

Shokoufeh Hassani ⁷

¹ Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

² Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

³ Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences (TUMS), Tehran, Iran

⁴ Department of toxicology and pharmacology, faculty of pharmacy,Tehran university of medical sciences,Tehran,Iran

⁵ Department of Toxicology and Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Hormozgan University of Medical Sciences

⁶ department of Pharmacognosy, Pharmaceutical sciences Branch, islamic Azad University, Tehran, Iran

⁷ Toxicology and Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), Tehran University of Medical Sciences (TUMS), Tehran, Iran.

10.22038/ajp.2024.25213

Abstract

Objective: Nasturtium officinale (N. officinale (NO)) has been widely used in traditional medicine. This study investigates the protective effects of NO against hepatic and renal damage induced by CCl₄ and gentamicin, respectively, in rats.
Materials and Methods: Male Wistar rats were divided into two arms: A (CCl4-induced hepatotoxicity) and B (gentamicin-induced nephrotoxicity). Seventeen groups were formed by dividing arms A and B, with nine groups in arm A and eight groups in arm B (n=5). Rats were daily treated with various doses (50, 100, and 200 mg/kg BW) of N. officinale extract (NOE) (Total extract; Oral gavage) for 14 and 28 days in arm A and B, respectively. Biochemical and histopathological evaluations and gene expression analyses were conducted on blood, liver, and kidney tissues.
Results: NOE treatment significantly modulated B-cell lymphoma protein 2 (Bcl-2)-associated X (Bax) and B-cell lymphoma protein 2 (Bcl-2) expression in kidney tissue, reducing Bax (p<0.01) and increasing Bcl-2 (p<0.05). In liver tissue, NOE inhibited tumor necrosis factor alpha (TNF-α) (p<0.01) and Interleukin-1 beta (IL-1β) (p<0.001), while reducing AST and ALT activity (p<0.001). Additionally, blood urea nitrogen (BUN) levels significantly decreased (p<0.05) in nephrotoxic rats.
Conclusion: Our findings highlight the capability of NOE as a promising therapeutic against liver and kidney damage induced by CCl₄ and gentamicin, respectively, in animal models.

Keywords

Nephrotoxicity

Hepatotoxicity

Nasturtium officinale

Inflammation

Apoptosis

Subjects