Document Type : Short communication
Authors
1
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
2
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
3
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4
Department of Pathology, Emam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
10.22038/ajp.2024.24786
Abstract
Objective: Contrast-induced nephropathy (CIN) raises the risk of renal injury, but crocin, a saffron component, may improve kidney function. This study investigated crocin's protective effects against CIN in rats.
Materials and Methods: Male Wistar rats were divided into eight groups: Sham, Control, Contrast medium (diatrizoate), Diatrizoate combined with crocin at 10, 20, or 40 mg/kg/day, Diatrizoate combined with N-acetylcysteine (NAC) at 125 mg/kg/day, and Crocin alone at 40 mg/kg/day. Water deprivation began on day 5 for 48 hr, except for the sham and crocin alone groups. Indomethacin and N(ω)-nitro-L-arginine methyl ester were administered after 40 hr of dehydration. Rats were sacrificed on the eighth day, and blood and kidney samples were collected.
Results: Diatrizoate increased serum creatinine and blood urea nitrogen levels, elevated malondialdehyde levels, and reduced glutathione in renal tissue. Crocin reversed these effects. Diatrizoate caused severe tubular necrosis, proteinaceous casts, medullary congestion, and interstitial edema in kidney tissue. Crocin (20 and 40 mg/kg) significantly reduced tubular necrosis, and doses of 10 and 40 mg/kg reduced interstitial edema. NAC significantly improved histopathological damage, biochemical factors, and oxidative stress. The crocin alone group showed no significant changes.
Conclusion: Diatrizoate induces nephrotoxicity by enhancing oxidative stress in rats, and crocin has a protective effect against it. Crocin mitigates both tissue and biochemical damage inflicted by diatrizoate.
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