Objective: Autophagy, as a cellular pathway involved in removing damaged proteins and organelles, performs a vital function in the homeostasis and fate of cells. Natural compounds of coumarin (CO) are found in a variety of herbs. Due to their many medicinal properties, including antitumor and antiproliferative activity, they are involved in apoptosis and autophagy processes. This investigation desired to analyze the apoptotic and autophagic effects of p-coumaric acid (PCA) and CO on HT-29 cells cultured in fibrin hydrogel. Materials and Methods: Cell viability and apoptotic and autophagic changes were evaluated by MTT assay, Acridine Orange, 4′,6-diamidino-2-phenylindole (DAPI), and monodansylcadaverine (MDC) staining. The expression Bax, Bad, Bcl2, Lc3, Beclin-1, P53 and Atg5 was respectively measured by qRT-PCR and Western blotting. Results:CO (IC50=25 μM) and PCA (IC50=150 μM) had a doseand time-dependent cytotoxic effect in HT-29 cells. So, the cytotoxic effects of CO were significantly higher than PCA and these differences were also evident in cell morphology investigations. The data illustrated a high expression of proapoptotic and pro-autophagic genes and a declined expression of anti-apoptotic and anti-autophagic genes. Conclusion: CO (that was more potent) and p-coumaric acidinduced autophagy via PI3K/Akt/mTOR and AMPK/mTOR signaling on HT-29 cells.
Hoveizi,E and Hushmandi,K . (2024). Comparison of effects of P-coumaric acid and coumarin on colorectal cancer cell line by inducing apoptosis and autophagy. Avicenna Journal of Phytomedicine, 14(4), 470-484. doi: 10.22038/ajp.2024.24194
MLA
Hoveizi,E , and Hushmandi,K . "Comparison of effects of P-coumaric acid and coumarin on colorectal cancer cell line by inducing apoptosis and autophagy", Avicenna Journal of Phytomedicine, 14, 4, 2024, 470-484. doi: 10.22038/ajp.2024.24194
HARVARD
Hoveizi E, Hushmandi K. (2024). 'Comparison of effects of P-coumaric acid and coumarin on colorectal cancer cell line by inducing apoptosis and autophagy', Avicenna Journal of Phytomedicine, 14(4), pp. 470-484. doi: 10.22038/ajp.2024.24194
CHICAGO
E Hoveizi and K Hushmandi, "Comparison of effects of P-coumaric acid and coumarin on colorectal cancer cell line by inducing apoptosis and autophagy," Avicenna Journal of Phytomedicine, 14 4 (2024): 470-484, doi: 10.22038/ajp.2024.24194
VANCOUVER
Hoveizi E, Hushmandi K. Comparison of effects of P-coumaric acid and coumarin on colorectal cancer cell line by inducing apoptosis and autophagy. Avicenna Journal of Phytomedicine. 2024;14(4):470-484. doi: 10.22038/ajp.2024.24194
