The cardioprotective properties of Persicaria maculosa and Citrus sinensis extracts against doxorubicin-induced cardiotoxicity in mice

Document Type : Original Research Article


1 Department of Chemistry, Faculty of Science, University of Kafrelsheikh, Kafr El-Sheikh, Egypt

2 Department of Chemistry, Faculty of Science, University of Mansoura, Mansoura, Egypt



 Objective: This study assessed the cardioprotective properties of
Persicaria maculosa (PME) and Citrus sinensis (CME) hydromethanolic extracts, besides Citrus sinensis aqueous extract
(CWE) against doxorubicin (DOX)-induced cardiotoxicity.
Materials and Methods: The extracts were characterized. Mice
were divided into eight groups: control (saline), DOX, protected
(injected with 200 mg/kg of PME, CWE or CME for 21 days,
orally, and DOX), and extracts (PME, CWE or CME
administration, orally, for 21 days). DOX was injected (5 mg/kg,
ip) on days 8, 13 and 18 of the experiment. Cardiac tumor necrosis
factor-alpha (
TNF-α), nuclear factor (erythroid-derived 2)-like 2
Nrf2) and carbonyl reductase 1 (CBR1) expression levels, besides
superoxide dismutase, catalase, malondialdehyde, nitric oxide and
total protein levels were evaluated. Serum lactate dehydrogenase,
creatine phosphokinase cardiac isoenzyme, aspartate transaminase,
cholesterol, triglycerides and creatinine levels, as well as the
cardiac tissues were examined.
Results: Comparing with the control, DOX considerably (p<0.01)
up-regulated TNF-α expression, malondialdehyde, nitric oxide,
cardiac enzymes, lipids and creatinine levels, while it significantly
(p<0.01) down-regulated Nrf2 and CBR1. Additionally, DOX
interfered with antioxidant enzymes' activities
Conversely, protected groups showed a significant
amelioration of DOX-induced cardiotoxic effects.
Conclusion: The current study provides a new understanding of P.
and C. sinensis cardioprotective mechanisms. The
extracts' cardioprotective effects may be due to their antioxidant
activities, ability to maintain the redox homeostasis through
regulation of important antioxidant genes and primary antioxidant
enzymes, and capability to recover inflammatory cytokines and
lipids levels. Noteworthy, the tested extracts showed no toxic
changes on the normal mice.


Main Subjects