Effects of gallic acid on intraperitoneal adhesion bands in rats

Document Type : Short communication

Authors

1 Department of Pharmacology and Toxicology, School of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran

2 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran

3 Department of Pathology, Faculty of Medicine, Zabol University of Medical Sciences, Zabol, Iran

4 International UNESCO Center for Health-Related Basic Sciences and Human Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

5 Department of Pharmacodynamics and Toxicology, School of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran

Abstract

Objective: Gallic acid (GA) is an organic acid that possesses anti-inflammatory effects as it inhibits the production of metalloproteinases, tissue plasminogen activator, growth factors and adhesion molecules. Since formation of abdominal surgery-induced adhesion bands is accompanied by inflammation, angiogenesis and cell proliferation, in the current study, we assessed potential beneficial properties of GA against adhesion bands formation in rats.
Materials and Methods: Thirty-six adult male rats were assigned into six groups of six animals. After induction of anesthesia, peritoneal injury was induced using a standard method and animals received either GA (10, 25, 50 and 100 mg/kg), or normal saline, while a group of rats remained intact. Seven days after the surgery, animals were decapitated and samples were collected for pathology evaluations. Also, lipid peroxidation (TBARS) and tumor necrosis factor alpha (TNF-α) levels were determined in serum samples.
Results: Our results showed that GA significantly reduced lipid peroxidation in serum samples but had no effect on TNF-α levels. Furthermore, microscopic and macroscopic injuries reduced significantly in GA-treated animals.
Conclusion: Since GA reduced adhesion bands formation at microscopic and macroscopic levels, it could be considered a treatment against adhesion bands formation.

Keywords


Aarons CB, Cohen PA, Gower A, Reed KL,
Leeman SE, Stucchi AF, Becker JM.
2007. Statins (HMG-CoA reductase
inhibitors) decrease postoperative
Gallic acid against adhesion bands
AJP, Vol. 12, No. 4, Jul-Aug 2022 343
adhesions by increasing peritoneal
fibrinolytic activity. Ann Surg, 245: 176.
Ara C, Kirimlioglu H, Karabulut AB, Coban
S, Hascalik S, Celik O, Yilmaz S,
Kirimlioglu V. 2005. Protective effect of
melatonin against oxidative stress on
adhesion formation in the rat cecum and
uterine horn model. Life Sci, 77: 1341-
1350.
Binda M, Molinas C, Koninckx P. 2003.
Reactive oxygen species and adhesion
formation Clinical implications in
adhesion prevention. Hum Reprod, 18:
2503-2507.
Brochhausen C, Schmitt VH, Planck CN,
Rajab TK, Hollemann D, Tapprich C,
Krämer B, Wallwiener C, Hierlemann H,
Zehbe R. 2012. Current strategies and
future perspectives for intraperitoneal
adhesion prevention. J Gastrointest Surg,
16: 1256-1274.
Cahill RA, Redmond HP. 2008. Cytokine
orchestration in post-operative peritoneal
adhesion formation. World J
Gastroenterol, 14: 4861.
Cheong Y, Laird S, Shelton J, Ledger W, Li
T, Cooke I. 2002. The correlation of
adhesions and peritoneal fluid cytokine
concentrations: a pilot study. Hum
Reprod, 17: 1039-1045.
Choi K-C, Lee Y-H, Jung MG, Kwon SH,
Kim M-J, Jun WJ, Lee J, Lee JM, Yoon
H-G. 2009. Gallic acid suppresses
lipopolysaccharide-induced nuclear
factor-κB signaling by preventing RelA
acetylation in A549 lung cancer cells.
Mol Cancer Res, 7: 2011-2021.
Demirbag S, Cetinkursun S, Tasdemir U,
Ozturk H, Pekcan M, Yesildaglar N.
2005. Comparison of
hyaluronate/carboxymethylcellulose
membrane and melatonin for prevention
of adhesion formation in a rat model.
Hum Reprod, 20: 2021-2024.
Devasagayam T, Sainis K. 2002. Immune
system and antioxidants, especially those
derived from Indian medicinal plants.
Indian J Exp Biol, 40: 639-655.
Diamond MP, El-Hammady E, Munkarah A,
Bieber EJ, Saed G. 2005. Modulation of
the expression of vascular endothelial
growth factor in human fibroblasts. Fertil
Steril, 83: 405-409.
Dubuisson J, Botchorishvili R, Perrette S,
Bourdel N, Jardon K, Rabischong B,
Canis M, Mage G. 2010. Incidence of
intraabdominal adhesions in a continuous
series of 1000 laparoscopic procedures.
Am J Obstet Gynecol, 203: 111.e1-3.
Eckes B, Nischt R, Krieg T. 2010. Cellmatrix interactions in dermal repair and
scarring. Fibrogenesis Tissue Repair, 3:
4.
Guvenal T, Cetin A, Ozdemir H, Yanar O,
Kaya T. 2001. Prevention of
postoperative adhesion formation in rat
uterine horn model by nimesulide: a
selective COX-2 inhibitor. Hum Reprod,
16: 1732-1735.
Hashemzaei AM, Badrooze BA, Tabrizian
CK, Shahraki EMK, Khatibani FEA,
Shahraki GJ. 2016. Effects of luteolin on
intra peritoneal adhesion bands in rat. J
Fundam Appl Sci, 8: 1-11.
Hashemzaei M, Abdollahzadeh M, Iranshahi
M, Golmakani E, Rezaee R, Tabrizian K.
2017a. Effects of luteolin and luteolinmorphine co-administration on acute and
chronic pain and sciatic nerve ligatedinduced neuropathy in mice. J
Complement Integr Med, 14.
Hashemzaei M, Entezari Heravi R, Rezaee R,
Roohbakhsh A, Karimi G. 2017b.
Regulation of autophagy by some natural
products as a potential therapeutic
strategy for cardiovascular disorders. Eur
J Pharmacol, 802: 44-51.
Hashemzaei M, Tabrizian K, Alizadeh Z,
Pasandideh S, Rezaee R, Mamoulakis C,
Tsatsakis A, Skaperda Z, Kouretas D,
Shahraki J. 2020. Resveratrol, curcumin
and gallic acid attenuate glyoxal-induced
damage to rat renal cells. Toxicol Rep, 7:
1571-1577.
Hassani FV, Rezaee R, Sazegara H,
Hashemzaei M, Shirani K, Karimi G.
2015. Effects of silymarin on neuropathic
pain and formalin-induced nociception in
mice. Iran J Basic Med Sci, 18: 715.
Heydrick SJ, Reed KL, Cohen PA, Aarons
CB, Gower AC, Becker JM, Stucchi AF.
2007. Intraperitoneal administration of
methylene blue attenuates oxidative
stress, increases peritoneal fibrinolysis,
and inhibits intraabdominal adhesion
formation. J Surg Res, 143: 311-319.
Hsiang C-Y, Hseu Y-C, Chang Y-C, Kumar
KS, Ho T-Y, Yang H-L. 2013. Toona
sinensis and its major bioactive
compound gallic acid inhibit LPS-
Hashemzaei et al.
AJP, Vol. 12, No. 4, Jul-Aug 2022 344
induced inflammation in nuclear factorκB transgenic mice as evaluated by in
vivo bioluminescence imaging. Food
Chem, 136: 426-434.
Inoue M, Suzuki R, Koide T, Sakaguchi N,
Ogihara Y, Yabu Y. 1994. Antioxidant,
gallic acid, induces apoptosis in HL60RG cells. Biochem Biophys Res
Commun, 204: 898-904.
Jomezadeh V, Mohammadpour AH, Rajabi
O, Tavassoli A, Maddah G. 2012.
Evaluation of curcumin effects on postoperative peritoneal adhesion in rats. Iran
J Basic Med Sci, 15: 1162.
Kaidi AA, Gurchumelidze T, Nazzal M,
Figert P, Vanterpool C, Silva Y. 1995.
Tumor necrosis factor-α: a marker for
peritoneal adhesion formation. J Surg
Res, 58: 516-518.
Kim S-H, Jun C-D, Suk K, Choi B-J, Lim H,
Park S, Lee SH, Shin H-Y, Kim D-K,
Shin T-Y. 2006. Gallic acid inhibits
histamine release and pro-inflammatory
cytokine production in mast cells.
Toxicol Sci, 91: 123-131.
Kroes B, Van den Berg A, Quarles vUH, Van
Dijk H, Labadie R. 1992. Antiinflammatory activity of gallic acid.
Planta Med, 58: 499-504.
Liakakos T, Thomakos N, Fine PM, Dervenis
C, Young RL. 2001. Peritoneal
adhesions: etiology, pathophysiology, and
clinical significance. Dig Surg, 18: 260-
273.
Mohammadpour AH, Tavassoli A, Khakzad
MR, Zibaee E, Afshar M, Hashemzaei M,
Karimi G. 2015. Effect of gold
nanoparticles on postoperative peritoneal
adhesions in rats. Nanomed J, 2: 211-216.
Moradi A, Abolfathi M, Javadian M,
Heidarian E, Roshanmehr H, Khaledi M,
Nouri A. 2020. Gallic acid exerts
nephroprotective, anti-oxidative stress,
and anti-inflammatory effects against
diclofenac-induced renal injury in
malerats. Arch Med Res, 52: 380-388.
Nair SK, Bhat IK, Aurora AL. 1974. Role of
proteolytic enzyme in the prevention of
postoperative intraperitoneal adhesions.
Arch Surg, 108: 849-853.
Newby AC. 2006. Matrix metalloproteinases
regulate migration, proliferation, and
death of vascular smooth muscle cells by
degrading matrix and non-matrix
substrates. Cardiovasc Res, 69: 614-624.
Newby AC, Zaltsman AB. 2000. Molecular
mechanisms in intimal hyperplasia. J
Pathol, 190: 300-309.
Ouaïssi M, Gaujoux S, Veyrie N, Denève E,
Brigand C, Castel B, Duron J, Rault A,
Slim K, Nocca D. 2012. Post-operative
adhesions after digestive surgery: their
incidence and prevention: review of the
literature. J Visc Surg, 149: e104-e114.
Özçelik B, Serin IS, Basbug M, Uludag S,
Narin F, Tayyar M. 2003. Effect of
melatonin in the prevention of post‐
operative adhesion formation in a rat
uterine horn adhesion model. Hum
Reprod, 18: 1703-1706.
Parsaei P, Karimi M, Asadi SY, Rafieiankopaei M. 2013. Bioactive components
and preventive effect of green tea
(Camellia sinensis) extract on postlaparotomy intra-abdominal adhesion in
rats. Int J Surg, 11: 811-815.
Pourmorad F, Hosseinimehr S, Shahabimajd
N. 2006. Antioxidant activity, phenol and
flavonoid contents of some selected
Iranian medicinal plants. Afr J
Biotechnol, 5: 1142-1145.
Saltzman AK, Olson TA, Mohanraj D,
Carson LF, Ramakrishnan S. 1996.
Prevention of postoperative adhesions by
an antibody to vascular permeability
factor/vascular endothelial growth factor
in a murine model. Am J Obstet Gynecol,
174: 1502-1506.
Üstün Y, Engin-Üstün Y, Ovayolu A,
Meydanli MM, Temel I, Kafkasli A.
2007. The effect of resveratrol on
prevention of the development of
postoperative adhesions in a rat model. J
Gynaecol Endocrinol, 23: 522-526.
Vykoukal D, Davies MG. 2011. Vascular
biology of metabolic syndrome. J Vasc
Surg, 54: 819-831.
Whang SH, Astudillo JA, Sporn E, Bachman
SL, Miedema BW, Davis W, Thaler K.
2011. In search of the best peritoneal
adhesion model: comparison of different
techniques in a rat model. J Surg Res,
167: 245-250.
Yilmaz HG, Tacyildiz IH, Keles C, Gedik E,
Kilinc N. 2005. Micronized purified
flavonoid fraction may prevent formation
of intraperitoneal adhesions in rats. Fertil
Steril, 84: 1083-1088.
Yoshida A, Anand-Apte B, Zetter BR. 1996.
Differential endothelial migration and
Gallic acid against adhesion bands
AJP, Vol. 12, No. 4, Jul-Aug 2022 345
proliferation to basic fibroblast growth
factor and vascular endothelial growth
factor. Growth Factors, 13: 57-64.
Zhang Y, Li X, Zhang Q, Li J, Ju J, Du N,
Liu X, Chen X, Cheng F, Yang L. 2014.
Berberine hydrochloride prevents
postsurgery intestinal adhesion and
inflammation in rats. J Pharmacol Exp
Ther, 349: 417-426.