Morphohistometric analysis of the effects of Coriandrum sativum on cortical and cerebellar neurotoxicity

Document Type : Original Research Article


Department of Anatomy, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia


Objective: Natural compounds can act as metal chelators and oxygen free radical scavengers, which allows them to be used as bioactive antagonists to heavy metals neurotoxicity. The aim of the study to analyze the morphometric effects of Coriandrum sativum (C. sativum) on lead-induced neurotoxicity.
Materials and Methods: Forty Sprague-Dawley albino rats were divided into four equal groups (ten in each group): control group; coriander group: received aqueous C. sativum extracts (600 mg/kg BW for 60 days orally); lead (Pb) group: received a daily dose of lead acetate (Pb) (10 mg/kg BW for 60 days orally); Pb+ coriandrum group: received: aqueous C. sativum extract (600 mg/kg BW) prior to 10 mg/kg BW of Pb. The following parameters malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were measured. Layers thickness and nuclei density were analyzed.
Results: Lead levels in blood and tissues were decreased significantly in the Pb group and those findings were corrected significantly (p=0.001) with C. sativum addition. Data exhibited an increase in oxidative stress marker MDA and a decrease in antioxidant enzymes activities (SOD, CAT, and GPx) significantly in the Pb group and those effects were reversed significantly (p=0.001) by C. sativum administration. The cerebellar cortex and all layers of the somatosensory cortex thickness and nuclei density were diminished significantly in the Pb group. The morphometrical measurements were corrected significantly (p=0.001) by C. sativum.
Conclusion: From the findings of the current study, Pb caused noticeable structural and functional variations in the cerebellar cortex and somatosensory cortex. C. sativum corrected these parameters as it possesses chelating and antioxidant potentials.


Al-Rubaye RHK. 2016. The inhibitory effect of
aqueous extract of Coriander (Coriandrum
sativum L.) leaves on the activity of male
reproductive system of albino mice. Iraqi J
Sci, 57: 344-351.
Donia GR. 2019. Protective effect of Coriander
(Corindrum sativum) against lead toxicity in
rabbits. Eur J Biomed, 6: 520-532.
El-aziz Tahoun EA, Mohammed RS, Donia
GR. 2018. Histopathological and
biochemical studies on the effect of green
tea extract and vitamin C against
fenitrothion toxicity in male Albino rats.
Alex J Vet Sci, 57: 74-86.
Ghosh S, Singha PS, Ghosh D. 2017. Leaves of
Coriandrum sativum as an indigenous
medicinal spice herb of India: A mini
review. Int J Pharmacol Sci Rev Res, 45:
Karami R, Hosseini M, Mohammadpour T,
Ghorbani A, Sadeghnia HR, Rakhshandeh
H, Vafaee F, Esmaeilizadeh M. 2015.
Effects of hydroalcoholic extract of
Coriandrum sativum on oxidative damage in
pentylenetetrazole-induced seizures in rats.
Iran J Neurol, 14: 59-66.
Leena K, Veena S, Arti S. 2011. Protective role
of Coriandrum sativum (coriander) extracts
against lead nitrate induced oxidative stress
and tissue damage in the liver and kidney in
male mice. Int J Appl Biol Pharm Technol,
2: 65-83.
Mehrandish R, Rahimian A, Shahriary A. 2019.
Heavy metals detoxification: A review of
herbal compounds for chelation therapy in
heavy metals toxicity. J Herbmed
Pharmacol, 8: 69-77.
Mustafa HN. 2020. Neuro-amelioration of
Cinnamaldehyde in aluminum-induced
alzheimer's disease rat model. J
Histotechnol, 43: 11-20.
Mustafa HN, Hussein AM. 2016. Does Allicin
combined with vitamin B-complex have
superior potentials than alpha-tocopherol
alone in ameliorating lead acetate-induced
purkinje cell alterations in rats? An
immunohistochemical and ultrastructural
study. Folia Morphol (Warsz), 75: 76-86.
Obafemi TO, Onasanya A, Adeoye A, Falode
JA, Daniel DJ, Irefo EF, Ojo AO, Fadaka A,
Afolabi OB, Awe JO. 2019. Protective
effect of methanolic and flavonoid-rich leaf
extracts of synsepalum dulcificum
(Danielli) on lead-acetate-induced toxicity
in wistar albino rats. J Appl Pharm Sci, 9:
Pourzaki M, Homayoun M, Sadeghi S,
Seghatoleslam M, Hosseini M,
Ebrahimzadeh Bideskan A. 2017.
Preventive effect of Coriandrum sativum on
neuronal damages in pentylentetrazole-
Mustafa et al.
AJP, Vol. 11, No. 6, Nov-Dec 2021 598
induced seizure in rats. Avicenna J
Phytomed, 7: 116-128.
Qadir MI, Ahmad Z. 2017. Advances in
hepatoprotective medicinal plants research.
Bangladesh J Pharmacol, 12: 229-242.
Ramezani R, Moghimi A, Rakhshandeh H,
Ejtehadi H, Kheirabadi M. 2008. The effect
of Rosa damascena essential oil on the
amygdala electrical kindling seizures in rat.
Pak J Biol Sci, 11: 746-751.
Saleh HA, Abd El-aziz GS, Mustafa HN, ElFark M, Mal A, Aburas M, Deifalla AH.
2019. Thymoquinone ameliorates oxidative
damage and histopathological changes of
developing brain neurotoxicity. J
Histotechnol, 42: 116-127.
Seghatoleslam M, Alipour F, Shafieian R,
Hassanzadeh Z, Edalatmanesh MA,
Sadeghnia HR, Hosseini M. 2016. The
effects of Nigella sativa on neural damage
after pentylenetetrazole induced seizures in
rats. J Tradit Complement Med, 6: 262-268.
Shin EJ, Jeong JH, Chung YH, Kim WK, Ko
KH, Bach JH, Hong JS, Yoneda Y, Kim HC.
2011. Role of oxidative stress in epileptic
seizures. Neurochem Int, 59: 122-137.
Shubina OS, Egorova MV, Komusova OI,
Dudenkova NA, Bardin VS, Labzina LY,
Labzina MV, Kirdyashkina OV. 2019.
Morphometric studies of the cerebral cortex
under exposure to lead acetate in albino rats.
Eurasia J Biosci, 13: 1791-1796.
Surkan PJ, Zhang A, Trachtenberg F, Daniel
DB, McKinlay S, Bellinger DC. 2007.
Neuropsychological function in children
with blood lead levels<10 μg/dL.
Neurotoxicology, 28: 1170-1177.
Tellez-Lopez MA, Mora-Tovar G, CenicerosMendez IM, Garcia-Lujan C, PuenteValenzuela CO, Vega-Menchaca MDC,
Serrano-Gallardo LB, Garza RG, MoranMartinez J. 2017. Evaluation of the
chelating effect of methanolic extract of
Coriandrum sativum and its fractions on
wistar rats poisoned with lead acetate. Afr J
Tradit Complement Altern Med, 14: 92-102.
Sharma V, Kansal L, Sharma A, Lodi SH,
Sharma SH. 2011. Ameliorating effect of
Coriandrum sativum extracts on
hematological and immunological variables
in an animal model of lead intoxication. J
Pharm Allied Health Sci, 1: 16-29.
Velaga MK, Yallapragada PR, Williams D,
Rajanna S, Bettaiya R. 2014.
Hydroalcoholic seed extract of Coriandrum
sativum (Coriander) alleviates lead-induced
oxidative stress in different regions of rat
brain. Biol Trace Elem Res, 159: 351-363.