Document Type : Original Research Article
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran
Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Objective: The aim of this study was to evaluate the anti-inflammatory effect of B. persicum essential oil on colonic inflammation and the role of suppression of NF-κB pathway in rat colitis induced by acetic acid solution.
Materials and Methods: Induction of acute colitis was done by intra-luminal instillation of 2 ml of acetic acid (4%) diluted in normal saline. Two hours after colitis induction, 0.2% tween 80 in normal saline, prednisolone (4 mg/kg) or B. persicum essential oil (100, 200, and 400 mg/kg) were administered to the rats orally and continued for 5 consecutive days. The severity of macroscopic and microscopic damages was assessed. Myeloperoxidase and TNF-α activity was evaluated by biochemical analysis and ELISA respectively and protein expression of p-NF-κB was assessed by immunohistochemistry (IHC).
Results: Prednisolone and B. persicum essential oil (100, 200, and 400 mg/kg) decreased macroscopic and microscopic injuries compared to the acetic acid group. On the other hand, prednisolone and B. persicum essential oil (200 and 400 mg/kg) decreased the activity of MPO and TNF-α in the colon tissue of rats compared with the acetic acid group. Furthermore, they suppressed the expression of p-NF-κB protein induced by acetic acid administration.
Conclusion: It is suggested that the anti-inflammatory effect of B. persicum essential oil on acetic acid-induced colitis in rats may be due to the suppression of NF-κB pathway.