The effect of royal jelly and silver nanoparticles on liver and kidney inflammation

Document Type : Short communication


1 Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences

2 Department of Immunology, Faculty of Medicine, Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

3 Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran

4 Department of Anatomy, Faculty of Medicine, Social Determinants of Health Research center Rafsanjan University of Medical Sciences, Rafsanjan, Iran

5 Department of Anatomy, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

6 Department of Anatomy, Faculty of Medicine, Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran


Objective: Royal jelly (RJ) is a honey bee product for which, anti-inflammatory properties were shown in vitro. Nanoparticles, including nano-silver (NS), are plausible inflammation inducers that act by activation of immune cells and consequent production of pro-inflammatory cytokines. This project aimed to explore immunomodulatory effects of royal jelly and nano-silver on the kidney and liver.
Materials and Methods: In this project, 40 male rats were grouped as follows: 10 rats as controls, 10 rats treated with RJ; 10 rats treated with both NS and RJ and 10 rats treated with NS. Liver and kidney interleukin (IL)-1β, -2, -6, and -33 levels were determined using commercial ELISA kits.
Results: RJ reduced kidney IL-6 levels in comparison to control and NS--RJ groups. RJ and NS reduced kidney and liver IL-1β levels. Kidney IL-33 levels were decreased in the RJ and nano-silver groups in comparison to the NS--RJ group.
Conclusion: Based on this study, it may be concluded that RJ together with NS can play anti-inflammatory roles and may affect the function of immune cells.


Ahmed MM, El-Shazly SA, Alkafafy ME,
Mohamed AA, Mousa AA. 2018.
Protective potential of royal jelly against
cadmium-induced infertility in male rats.
Andrologia, 50: e12996.
Au-Yeung BB, Smith GA, Mueller JL, Heyn
CS, Jaszczak RG, Weiss A, Zikherman J.
2017. IL-2 Modulates the TCR Signaling
Threshold for CD8 but Not CD4 T Cell
Proliferation on a Single-Cell Level. J
Immunol, 198: 2445-2456.
Borthwick LA. 2016. The IL-1 cytokine family
and its role in inflammation and fibrosis in
the lung. Semin Immunopathol, 38: 517-
Delkhoshe-Kasmaie F, Malekinejad H,
Khoramjouy M, Rezaei-Golmisheh A
&Janbaze-Acyabar H. 2014. Royal jelly
protects from taxol-induced testicular
damages via improvement of antioxidant
status and up-regulation of E2f1. Syst Biol
Reprod Med, 60: 80-88.
El-Gayar MH, Aboshanab KM, Aboulwafa M
M, Hassouna NA. 2016. Antivirulence and
wound healing effects of royal jelly and
garlic extract for the control of MRSA skin
infections. Wound Med, 13: 18-27.
Kamakura M. 2011. Royalactin induces queen
differentiation in honeybees. Nature, 473:
Klingelfus T, Disner G R, Voigt CL, Alle LF,
Cestari MM, Leme DM. 2019.
Nanomaterials induce DNA-protein
crosslink and DNA oxidation: A
mechanistic study with RTG-2 fish cell line
and Comet assay modifications.
Chemosphere, 215: 703-709.
Locht LJ, Pedersen MO, Markholt S, Bibby B
M, Larsen A, Penkowa M, Stoltenberg M
Rungby J. 2011. Metallic silver fragments
cause massive tissue loss in the mouse
brain. Basic Clin Pharmacol Toxicol, 109:
Metwally Ibrahim SL, Kosba AA. 2018. Royal
jelly supplementation reduces skeletal
Nano-silver, royal jelly and cytokines
AJP, Vol. 11, No. 3, May-Jun 2021 223
muscle lipotoxicity and insulin resistance in
aged obese rats. Pathophysiology, 25: 307-
Nakkala JR, Mata R, Raja K, Khub Chandra V,
Sadras SR. 2018. Green synthesized silver
nanoparticles: Catalytic dye degradation, in
vitro anticancer activity and in vivo toxicity
in rats. Mater Sci Eng C Mater Biol Appl,
91: 372-381.
Nakkala JR, Mata R, Sadras SR. 2017. Green
synthesized nano silver: Synthesis,
physicochemical profiling, antibacterial,
anticancer activities and biological in vivo
toxicity. J Colloid Interface Sci, 499: 33-45.
Nascimento AP, Moraes L. A, Ferreira NU,
Moreno Gde P, Uahib FG, Barizon EA ,
Berretta AA. 2015. The Lyophilization
Process Maintains the Chemical and
Biological Characteristics of Royal Jelly.
Evid Based Complement Alternat Med,
2015: 825068.
Pavel CI, Mărghitaş L A, Bobiş O, Dezmirean
DS, Şapcaliu A, Radoi I , Mădaş MN. 2011.
Biological activities of royal jelly-review.
Sci Papers Anim Sci Biotec, 44: 108-118.
Rahmanzadeh-Shahi S, Golshiri-Isfahani A,
Fathollahi MS, Rezayati M, Bahramabadi
R, Afrooz M, Asadikaram G., Kennedy D,
Arababadi MK. 2018. Interleukin-6 and
Tumor Growth Factor-beta are Risk Factors
for Idiopathic Epistaxis. Lab Med, 49: 329-
Ramadan MF , Al-Ghamdi A. 2012. Bioactive
compounds and health-promoting
properties of royal jelly: A review. J funct
Food, 4: 39-52.
Sepehri Z, Kiani Z, Afshari M, Kohan F,
Dalvand A, Ghavami S. 2017.
Inflammasomes and type 2 diabetes: An
updated systematic review. Immunol Lett,
192: 97-103.
Sharma VK, Yngard RA , Lin Y. 2009. Silver
nanoparticles: green synthesis and their
antimicrobial activities. Adv coll interface
sci, 145: 83-96.
Shin SH, Ye MK, Kim HS , Kang HS. 2007.
The effects of nano-silver on the
proliferation and cytokine expression by
peripheral blood mononuclear cells. Int
Immunopharmacol, 7: 1813-8.
Silici S, Ekmekcioglu O, Eraslan G , Demirtas
A. 2009. Antioxidative effect of royal jelly
in cisplatin-induced testes damage.
Urology, 74: 545-51.
Song JY , Kim BS. 2009. Rapid biological
synthesis of silver nanoparticles using plant
leaf extracts. Bioproces biosystem
engineer, 32: 79.
Tong X, Barbour M, Hou K, Gao C, Cao S,
Zheng J, Zhao Y, Mu R , Jiang HR. 2016.
Interleukin-33 predicts poor prognosis and
promotes ovarian cancer cell growth and
metastasis through regulating ERK and
JNK signaling pathways. Mol Oncol, 10:
Wang JJ. Xue YY, Tang M. 2016.
[Antimicrobial applications and toxicity of
nano-silver in the medical field]. Zhonghua
Shao Shang Za Zhi, 32: 631-634.
You MM, Chen YF, Pan Y M, Liu YC, Tu J,
Wang K , Hu FL. 2018. Royal Jelly
Attenuates LPS-Induced Inflammation in
BV-2 Microglial Cells through Modulating
NF-kappaB and p38/JNK Signaling
Pathways. Mediators Inflamm, 2018: