The effect of ginger (Zingiber officinale) feed on cardiac biomarker in medium-dose isoproterenol-induced myocardial toxicity

Document Type : Short communication


1 Department of Physiology, College of Health Sciences, Bowen University, Iwo Nigeria

2 Department of Physiological Sciences, Faculty of Basic Medical Sciences, Obafemi Awolowo University, Ile- Ife


Objectives- Traditional medicines have been widely used to prevent and treat diseases for thousands of years. This study was designed to evaluate the effect of ginger feed on cardiac biomarker in isoproterenol induced myocardial toxicity.
Materials and Methods- Thirty male wistar rats were grouped into six groups of 5 rats each: Control; ISO- induced toxicity; ginger fed ; ginger fed before; ginger fed+ isoproterenol simultaneously and ginger fed after. Freshly prepared solution of isoproterenol was injected subcutaneously at a dosage of 20mg/kg, while the control recieved distilled water. Blood was collected via cardiac puncture after two weeks of administration, the serum was used to evaluate biomarkers.
Results- The CK-MB of ginger fed groups was significantly lower (p < 0.05) compared to ISO group(8.2 ± 0.5µ/L). The CK of the ginger fed groups showed significant decrease (p>0.05) compared to isoproterenol group (39.36±5.28 µ/L), there was no significant difference in the CK-MB and CK levels of all the groups fed with ginger compared to the control(2.2± 0.3µ/L; 17. 07 ± 3.4.90 µ/L) except the group that was fed with ginger after isoproterenol induction, which was significantly higher compared to the control(p>0.05). The mean value of LDH were lower in all ginger treated groups compared to the ISO group (67.17± 0.88; p≤ 0.05), but significantly higher (p < 0.05) compared to the control(26.45 ± 2.52). The mean value of ALT were lower in all ginger fed groups compared to the ISO group (83.11± 4.88; p ≤ 0.05).
Conclusion- Ginger feed hindered toxic effects of isoproterenol.


National Research Council. 2011. (US)
Committee for the Update of the Guide for
the Care and Use of Laboratory
Animals.Washington (DC): National
Academies Press (US); .8th edition.
Apple FS. 1992. Diagnostic markers for
detection of acute myocardial infarction and
reperfusion. Lab Med, 23: 297-322.
Heraldo GLF, Nestor LF, Rafael BS, Eduardo
RC, Patrícia LDL. 2011. Experimental
model of myocardial infarction induced by
isoproterenol in rats. Rev Bras Cir
Cardiovasc, 26:469-476
Acikel M, Buyukokuroglu ME, Erdogan F,
Aksoy H, Bozkurt E, Senocak H. 2005.
Protective effects of dantrolene against
myocardial injury induced by isoproterenol
in rats: biochemical and histological
findings. Int J Cardiol, 98: 389-94.
Ojha S,Jagriti B, Sachin A, Mahaveer G,
Santosh K, Dharamvir SA. 2011.
Cardioprotective effects of Commiphora
mukul against isoprenaline-induced
cardiotoxicity: A biochemical and
istopathological evaluation. J Environ Biol,
32: 731-738.
Adams JE, Abendschein DR, Jaffe AS .1993.
Biochemical markers of myocardial injury:
Is MB Creatine kinase the choice for the
1990? Circulation, 88: 750-763.
Murray CJL, LopeA DZ. 1997. Alternative
projections of mortality and disability by
cause 1990–2020: Global Burden of Disease
Study. Lancet, 349: 1498–1504.
Rajadurai M, Prince P.S. (2007): “Preventive
effect of narginin on cardiac mitochondrial
enzymes during isoproterenol-induced
myocardial infarction in rats: a transmission
electron microscopic study. J of Biochem
Ginger feed attenuates iso-induced toxicity
AJP, Vol. 11, No. 1, Jan-Feb 2021 9
and Molecu Toxicol, 21: 354–361.
Saravanan G, Ponmurugan P, Sathiyavathi M,
Vadivukkarasi S, Sengottuvelu S. 2013.
Cardioprotective activity of Amaranthus
viridis Linn: effect on serum marker
enzymes, cardiac troponin and antioxidant
system in experimental myocardial infarcted
rats. Int J Cardio, 165: 494–498.
Priscilla DH, Prince PSM. 2009.
Cardioprotective effect of gallic acid on
cardiac troponin-T, cardiac marker
enzymes, lipid peroxidation products and
antioxidants in experimentally induced
myocardial infarction in Wistar rats,”
Chemico-Biol Interact,179: 118–124.
Amano S, Arai M, Goto S, Togari A. 2007.
Inhibitory effect of NPY on isoprenalineinduced osteoclastogenesis in mouse bone
marrow cells. Biochem Biophys Acta, 1770:
966- 973.
Helal EGE, El-Wahab SMA, Sharaf AMM,
Zedan GA. 2012. Effect of Zingiber
officinale on fatty liver induced by
oxytetracycline in albino rats. Egyp J Hosp
Med, 46: 26-42.
Wang Q, Xiao-feng Y, Hua-li X, Yi-chuan J,
Xue-zhong Z, and Da-yuan S. 2018.
Ginsenoside Re Attenuates IsoproterenolInduced Myocardial Injury in Rats
Evidence-Based Compl Alter Med, 2018: 1-
Tasatargil A, Kuscu N, Dalaklioglu S. 2017.
Cardioprotective effect of nesfatin-1 against
isoproterenol-induced myocardial infarction
in rats: Role of the Akt/GSK-3𝛽 pathway.
Peptides, 95: 1–9.
Peer PA, Trivedi PC, Nigade PB, Ghaisas MM,
Deshpande AD. 2008 Cardioprotective
effect of Azadirachta indica A. Juss. on
isoprenaline induced myocardial infarction
in rats. Int J Cardiol, 26: 123-126.
Akila P, Asaikumar L, Vennila L. 2017.
Chlorogenic acid ameliorates isoproterenolinduced myocardial injury in rats by
stabilizing mitochondrial and lysosomal
enzymes. Biomed Pharmacother. 85: 582-
Shukla Y, Singh M. 2007. Cancer preventive
properties of ginger: a brief review. Food
Chem Toxicol; 45: 683-90.
Afzal M, Al-hadidi D, Menon M, Pesek J,
Dhami MS. 2011. Ginger: An
Ethnomedical, Chemical and
Pharmacological Review. Drug Interact, 18:
Duke JA, Ayensu ES. 1997. Medicinal Plants
of China. Medicinal Plants of the World.
Algonac, MI: Reference Publications, USA,
pp. 362. Ithayarasi AP, Devi CS. Indian J
Physiol Pharmacol, 41: 369-376.
Glick MR, Ryder KW, Jackson SA. 1986.
Graphical Comparisons of Interferences in
Clinical Chemistry lnstrumentation. Clin
Chem, 32: 470-474.
Athirah ZA, Ibrahim J, Roza D, and Fhataheya
B. Protective effects of the standardized
extract of Zingiber officinale on
myocardium against isoproterenol-induced
biochemical and histopathological
alterations in rats. Pharm Biol, 53: 1795-
Duan L, Xiong X, Hu J, Liu Y, Li J, and Wang
J. 2017. “Panax notoginseng saponins for
treating coronary artery disease: A
functional and mechanistic overview,”
Frontiers Pharmacol, 8: 702.
Dugasani S, Pichika MR, Nadarajah VD,
Balijepalli MK, Tandra S, Korlakunta JN.
Comparative antioxidant and antiinflammatory effectsof [6]-gingerol, [8]-
gingerol, [10]-gingerol and [6]-shogaol. J
Ethnopharmacol, 2010; 127: 515-20.
Shanmugam KR, Mallikarjuna K, Sathyavelu
Reddy K. Efficacy of ethanolic extract of
ginger on kidney lipid metabolic profiles in
diabetic rats. Int J Diabetes Dev Ctries,
2011; 31: 97-103.
Ansari MN, Bhandari U, Pillai KK. 2006.
Ethanolic Zingiber officinale R extract pretreatment alleviates isoproterenol-induced
oxidative myocardial necrosis in rats. I J
Expl Bio, 44: 892-7.
Raish M. 2017. Momordica charantia
polysaccharides ameliorate oxidative stress,
hyperlipidemia, inflammation, and
apoptosis during myocardial infarction by
inhibiting the NF-κB signaling pathway. Int
J Biol Macromol; 97: 544-551.
Roth GA, Abate D, Abate KH et al.(2018).
Global, regional, and national age-sexspecific mortality for 282 causes of death in
195 countries and territories, 1980–2017: a
systematic analysis for the Global Burden of
Disease Study 2017. Lancet. 392: 1736-
Woudstra L, Biesbroek PS, Emmens RW,
Heymans S, Juffermans LJ, van Rossum
AC. 2017. Lymphocytic myocarditis occurs
Olumide Ojo et al.
AJP, Vol. 11, No. 1, Jan-Feb 2021 10
with myocardial infarction and coincides
with increased inflammation, hemorrhage
and instability in coronary artery
atherosclerotic plaques. Int J Cardio, 232:
Long J, Gao M, Kong Y, Shen X, Du X, Son
YO, et al. 2012. Cardioprotective effect of
total paeony glycosides against
isoprenaline-induced myocardial ischemia
in rats. Phytomedicine, 19: 672-676.
Awada HK, Johnson NR, Wang Y. Sequential
delivery of angiogenic growth factors
improves revascularization and heart
function after myocardial infarction. J
Contr. Relea, 2015; 207: 7-17.
Subbaiah GV, Mallikarjuna K, Shanmugam B,
Ravi S, Taj PU, Reddy KS. 2017. Ginger
treatment ameliorates alcohol-induced
myocardial damage by suppression of
hyperlipidemia and cardiac biomarkers in
rats. Phcog Mag, 13: 69-75.
Kaplan, L.A., Pesce, AJ. 1989. Clinical
Chemistry, St. Louis, C.V. Mosby, pp. 911-
Mansour A, Bakheet SA, Aleisa AM, Al-Rejaie
SS, AL-Yahya AA, ElAmeen M, et al.
Protective Effect of 6-Gingerol Against
cardiotoxicity Induced by Doxorubicin.
Open Pharmacol J, 2: 20-3.
Attyah AM, Ismail SH. 2008. Protective effect
of ginger extract against cisplatin-induced
hepatotoxicity and cardiotoxicity in rats.
Iraqi J Pharm Sci, 2012; 21: 27-33.
Ansari MN, Bhandari U, Pillai KK. 2006.
Ethanolic Zingiber officinale R extract pretreatment alleviates isoproterenol-induced
oxidative myocardial necrosis in rats. I J
Expl Bio; 44: 892-7.
Siddiqui MA, Ahmad U, Khan AA,
Badruddeen AM, Khalid M, Akhtar J. 2016.
Isoprenaline: A Tool for Inducing
Myocardial Infarction in Experimental
Animals. Int J Pharm. 6: 138-144.
Whellan DJ. 2005. Heart failure disease
management: implementation and
outcomes. Cardiol Rev, 13: 231-39.
McGill R.M. (2016). The past and present of
serum aminotransferases and the future of
liver injury biomarkers. EXCLI J, 15: 817–
Krushna G, Kareem MA, Devi KL. 2009. Antidyslipidaemic effect of Aegle marmelos
Linn. Fruit on isoproterenol induced
myocardial injury in rats. Int J Pharmacol, 6:
Tietz, NW. 1982. Fundamentals of Clinical
Chemistry, W.B. Saunders co., p 674.
Committee on Enzymes of the Scandinavian
Society for Clinical Chemistry and Clinical
Physiology. 1974. Scand J Clin Lab Invest,
United Nations(2015).Transforming our world:
the 2030 agenda for sustainable
Zhang GX, Kimura S, Nishiyama A, Shokoji T,
Rahman M, Yao L, Nagai Y, Fujisawa Y,
Miyatake A, Abe Y. (2005). Cardiac
oxidative stress in acute and chronic
isoproterenol-infused rats. Cardiovasc Res,
65: 230-238.