Document Type: Original Research Article
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R.Iran.
School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences
Objective: Colitis is an inflammatory bowel disease with unknown etiology where many factors might play a role. Adiantum capillus-veneris mayhave beneficial effects in colitis because of its anti-inflammatory, antioxidant, wound healing and antimicrobial effects. The aim of this study was to explore the anti-inflammatory and anti-ulcerative effects of A. capillus-veneris on acetic acid-induced colitis in a rat model.
Materials and Methods: A. capillus-veneris aqueous (ACAE; 150, 300, and 600 mg/kg) and hydroalcoholic extract (ACHE; 150, 300, and 600 mg/kg) were given orally (p.o.) to male Wistar rats 2 hr before induction of colitis by intra-rectal administration of acetic acid 3%, and continued for 4 days. Prednisolone (4 mg/kg) and mesalazine (100 mg/kg) were applied p.o., as reference drugs for comparison. On day five, colitis indices of tissue specimens were evaluated and levels of biochemical markers including myeloperoxidase (MPO) and malondialdehyde (MDA) were determined.
Results: In all groups treated with ACAE and ACHE with the exception of ACAE (150 mg/kg), ulcer index and wet weight of colon as parameters of macroscopic injuries, total colitis index as marker of microscopic features and MPO activity were significantly reduced in comparison to the control group; however, MDA value was only diminished in ACAE (300 and 600 mg/kg) and ACHE (300 mg/kg) groups significantly.
Conclusion: This research showed that ACAE and ACHE had dose-related beneficial effects on acetic acid-induced colitis and these effects could be attributed to anti-inflammatory, ulcer healing and antioxidant activities of these extracts.