Cinnamon extract supplementation improves inflammation and oxidative stress induced by acrylamide: An experimental animal study

Document Type : Original Research Article


1 Nutrition and Metabolic Diseases Research Center, Department of Nutrition, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

2 Hyperlipidemia Research Center, Department of Nutrition, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

3 Department of Nutrition, Faculty of Paramedicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

4 Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.


Objective: Toxic effects of acrylamide on body organs incline researches to prevent or decrease these effects. The objective of the present study was to evaluate the effects of cinnamon extract (CE) supplementation on inflammation and oxidative stress induced by acrylamide in rats.
Materials and Methods: Thirty two rats were divided into four groups as follow 1) The control group received distilled water, 2) Acrylamide- intoxicated group was administrated with 35 ml/kg/day acrylamide for two weeks, 3) Acrylamide- intoxicated rats treated with CE 250 mg/kg/day for 28 days, and 4) Acrylamide- intoxicated rats treated with CE 500 mg/kg day for 28 days. Fasting blood sample was obtained for subsequent analysis.
Results: The results showed that acrylamide- intoxicated group had significantly higher levels of malondialdehyde, tumor necrosis factor alpha, high sensitive C reactive protein, leptin and alanine transaminase (p0.05).
Conclusion: This study suggests that cinnamon extract may potentially be effective as a dietary source of bioactive compounds for managing acrylamide intoxication.


Main Subjects

Ansar S, Siddiqi NJ, Zargar S, Ganaie MA, Abudawood M. 2016. Hepatoprotective effect of Quercetin supplementation against Acrylamide-induced DNA damage in wistar rats. BMC Complement Altern Med,16:327-332.
Alturfan AA, Tozan-Beceren A, Şehirli AÖ, Demiralp E, Şener G, Omurtag GZ. 2012. Resveratrol ameliorates oxidative DNA damage and protects against acrylamide-induced oxidative stress in rats. Mol Biol Rep, 39:4589-4596.
Askari F, Rashidkhani B, Hekmatdoost A. 2014. Cinnamon may have therapeutic benefits on lipid profile, liver enzymes, insulin resistance, and high-sensitivity C-reactive protein in nonalcoholic fatty liver disease patients. Nutr Res, 34:143-148.
Amin KA, El-Twab A. 2009. Oxidative markers, nitric oxide and homocysteine alteration in hypercholesterolimic rats: role of atorvastatine and cinnamon. Int J Clin Exp Med, 2 :254-265.
Braithwaite I, Stewart AW, Hancox RJ, Beasley R, Murphy R, Mitchell EA, ISAAC Phase Three Study Group; ISAAC Phase Three Study Group. 2014. Fast-food consumption and body mass index in children and adolescents: an international cross-sectional study. BMJ Open, 4:e005813.
Cao H, Polansky MM, Anderson RA. 2007. Cinnamon extract and polyphenols affect the expression of tristetraprolin, insulin receptor, and glucose transporter 4 in mouse 3T3-L1 adipocytes. Arch Biochem Biophys, 459: 214-222.
Dehghan G, Shaghaghi M, Jafari A, Mohammadi M, Badalzadeh R. 2014. Effect of endurance training and cinnamon supplementation on post-exercise oxidative responses in rats. Mol Biol Res Commun, 3:269-281.
Einstein A, Podolsky B, Rosen N. 1935. Can quantum-mechanical description of physical reality be considered complete? Phys Rev, 47: 777-780.
El-Baroty GS, El-Baky HA, Farag R, Saleh MA. 2010. Characterization of antioxidant and antimicrobial compounds of cinnamon and ginger essential oils. AJBR, 4: 167-174.
Eidi A, Mortazavi P, Bazargan M, Zaringhalam J. 2012. Hepatoprotective activity of cinnamon ethanolic extract against CCL 4-induced liver injury in rats. EXCLI J, 11:495-507.
El-Mehi AE, El-Sherif NM. 2015. Influence of acrylamide on the gastric mucosa of adult albino rats and the possible protective role of rosemary. Tissue Cell, 47: 273-283.
El‐Raouf A, Ola M, El‐Sayed ESM, Manie MF. 2015. Cinnamic acid and cinnamaldehyde ameliorate cisplatin‐induced splenotoxicity in rats. J Biochem Mol Toxicol, 29: 426-431.
Ejaz A, Wu D, Kwan P, Meydani M. 2009. Curcumin inhibits adipogenesis in 3T3-L1 adipocytes and angiogenesis and obesity in C57/BL mice. J Nutr, 2009;139: 919-925.
Goffeng LO, Kjuus H, Heier MS, Alvestrand M, Ulvestad B, Skaug V. 2008. Colour vision and light sensitivity in tunnel workers previously exposed to acrylamide and N-methylolacrylamide containing grouting agents. Neurotoxicology, 29: 31-39.
Ghobadi Pour M, Mirazi N, Seif A. 2019. Treatment of liver and spleen illnesses by herbs: Recommendations of Avicenna’s heritage "Canon of Medicine". Avicenna J Phytomed, 9:101-116.
Ghorbel I, Chaabane M, Elwej A, Kallel C, Grati Kamoun N, Najiba Z. 2017. Extra Virgin olive oil mitigates hematotoxicity induced by acrylamide and oxidative damage in adult rats. Mazums-pbr, 3 :34-40.
Gaique TG, Lopes BP, Souza LL, Paula GS, Pazos‐Moura CC, Oliveira KJ. 2016. Cinnamon intake reduces serum T3 level and modulates tissue‐specific expression of thyroid hormone receptor and target genes in rats. J Sci Food Agric, 96: 2889-2895.
Gunawardena D, Karunaweera N, Lee S, van Der Kooy F, Harman DG, Raju R, Bennett L, Gyengesi E, Sucher NJ, Münch G. 2015. Anti-inflammatory activity of cinnamon (C. zeylanicum and C. cassia) extracts–identification of E-cinnamaldehyde and o-methoxy cinnamaldehyde as the most potent bioactive compounds. Food Funct, 6: 910-919.
Hansen SH, Olsen AK, Søderlund EJ, Brunborg G. 2010. In vitro investigations of glycidamide-induced DNA lesions in mouse male germ cells and in mouse and human lymphocytes. Mutat Res, 696: 55-61.
Hagenlocher Y, Hösel A, Bischoff SC, Lorentz A. 2016. Cinnamon extract reduces symptoms, inflammatory mediators and mast cell markers in murine IL-10−/− colitis. J Nutr Biochem, 30:85-92.
Haidari F, Omidian K, Rafiei H, Zarei M, Mohamad Shahi M. 2013. Green tea (Camellia sinensis) supplementation to diabetic rats improves serum and hepatic oxidative stress markers. Iran J Pharm Res, 12: 109-114.
Hong J-W, Yang G-E, Kim YB, Eom SH, Lew J-H, Kang H. 2012. Anti-inflammatory activity of cinnamon water extract in vivo and in vitro LPS-induced models. BMC Complement Altern Med, 12: 1-8.
Heber D, Zhang Y, Yang J, Ma JE, Henning SM, Li Z. 2014. Green tea, black tea, and oolong tea polyphenols reduce visceral fat and inflammation in mice fed high-fat, high-sucrose obesogenic diets. J Nutr, 144: 1385-1393.
Ismail NS. 2014. Protective effects of aqueous extracts of cinnamon and ginger herbs against obesity and diabetes in obese diabetic rat. WJDFS, 9: 145-153.
Jiang L, Cao J, An Y, Geng C, Qu S, Jiang L, Zhong L. 2007. Genotoxicity of acrylamide in human hepatoma G2 (HepG2) cells. Toxicol In Vitro, 21: 1486-1492.
Kokanova-Nedialkova Z, Nedialkov P, Kondeva-Burdina M, Simenova R, Tsankova V, Nikolov S. 2016. Good king-henry (Chenopodium bonus-henricus L.)–a source of hepatoprotective flavonoids. Planta Med, 81: 840-841.
Koochaksaraie R, Irani M, Gharavysi S. 2011. The effects of cinnamon powder feeding on some blood metabolites in broiler chicks. Rev Bras Cienc Avic, 13: 197-202.
Lasekan O, Abbas K. 2010. Analysis of volatile flavour compounds and acrylamide in roasted Malaysian tropical almond (Terminalia catappa) nuts using supercritical fluid extraction. Food Chem Toxicol, 48: 2212-2216.
Liu S, Jiang L, Zhong T, Kong S, Zheng R,Kong F, Zhang C, Zhang L, An L. 2015. Effect of Acrylamide on Oocyte Nuclear Maturation and Cumulus Cells Apoptosis in Mouse In Vitro. PloS One, 10:e0135818.
Li RW, Theriault AG, Au K, Douglas TD, Casaschi A, Kurowska EM, Mukherjee R. 2006. Citrus polymethoxylated flavones improve lipid and glucose homeostasis and modulate adipocytokines in fructose-induced insulin resistant hamsters. Life Sci, 79: 365-373.
Lu T, Sheng H, Wu J, Cheng Y, Zhu J, Chen Y. 2012. Cinnamon extract improves fasting blood glucose and glycosylated hemoglobin level in Chinese patients with type 2 diabetes. Nutr Res, 32: 408-412.
Mroueh M, Saab Y, Rizkallah R. 2004. Hepatoprotective activity of Centaurium erythraea on acetaminophen‐induced hepatotoxicity in rats. Phytother Res, 18: 431-433.
Moselhy SS, Ali HK. 2009. Hepatoprotective effect of cinnamon extracts against carbon tetrachloride induced oxidative stress and liver injury in rats. Biol Res, 42: 93-98.
Machado MV, Coutinho J, Carepa F, Costa A, Proença H, Cortez-Pinto H. 2012. How adiponectin, leptin, and ghrelin orchestrate together and correlate with the severity of nonalcoholic fatty liver disease. Eur J Gastroenterol Hepatol, 24: 1166-1172.
Matsunami T, Sato Y, Ariga S, Sato T, Kashimura H, Hasegawa Y, Yukawa M. 2010. Regulation of oxidative stress and inflammation by hepatic adiponectin receptor 2 in an animal model of nonalcoholic steatohepatitis. Int J Clin Exp Pathol, 3: 472-481.
Mahmoud AM. 2013. Hematological alterations in diabetic rats—role of adipocytokines and effect of citrus flavonoids. EXCLI J, 12:647-657.
Ohashi K, Parker JL, Ouchi N, Higuchi A, Vita JA, Gokce N, Pedersen AA, Kalthoff C, Tullin S, Sams A, Summer R, Walsh K. 2010. Adiponectin promotes macrophage polarization toward an anti-inflammatory phenotype. J Biol Chem, 285: 6153-6160.
Papoušek R, Pataj Z, Nováková P, Lemr K, Barták P. 2014. Determination of acrylamide and acrolein in smoke from tobacco and E-cigarettes. Chromatographia, 77: 1145-1151.
Roussel A-M, Hininger I, Benaraba R, Ziegenfuss TN, Anderson RA. 2009. Antioxidant effects of a cinnamon extract in people with impaired fasting glucose that are overweight or obese. J Am Coll Nutr, 28: 16-21.
Raj VP, Chandrasekhar RH, Vijayan P, Dhanaraj S, Rao MC, Rao VJ, Nitesh K. . 2010. In vitro and in vivo hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata leaves. Indian J Pharmacol, 42: 99-104.
Septembre‐Malaterre A, Le Sage F, Hatia S, Catan A, Janci L, Gonthier MP. 2016. Curcuma longa polyphenols improve insulin‐mediated lipid accumulation and attenuate proinflammatory response of 3T3‐L1 adipose cells during oxidative stress through regulation of key adipokines and antioxidant enzymes. Biofactors, 8: 418-430.
Shen Y, Wang Q, Zhao Q, Zhou J. 2009. Leptin promotes the immune escape of lung cancer by inducing proinflammatory cytokines and resistance to apoptosis. Mol Med Rep, 2: 295-299.
Shatwan IA, Ahmed LA, Badkook MM. 2013. Effect of barley flour, crude cinnamon, and their combination on glycemia, dyslipidemia, and adipose tissue hormones in type 2 diabetic rats. J Med Food, 16: 656-662.
Shihabudeen HMS, Priscilla DH, Thirumurugan K. 2011. Cinnamon extract inhibits α-glucosidase activity and dampens postprandial glucose excursion in diabetic rats. Nutr Metab (Lond), 8: 46-57.
Taubert D, Glöckner R, Müller D, Schömig E. 2006. The garlic ingredient diallyl sulfide inhibits cytochrome P450 2E1 dependent bioactivation of acrylamide to glycidamide. Toxicol Lett, 164: 1-5.
Tang H, Sebastian BM, Axhemi A, Chen X, Hillian AD, Jacobsen DW, Nagy LE. 2012. Ethanol‐induced oxidative stress via the CYP2E1 pathway disrupts adiponectin secretion from adipocytes. Alcohol Clin Exp Res, 36: 214-22.
Watzek N, Scherbl D, Schug M, Hengstler JG, Baum M, Habermeyer M, Richling E, Eisenbrand G. 2013. Toxicokinetics of acrylamide in primary rat hepatocytes: coupling to glutathione is faster than conversion to glycidamide. Arch Toxicol, 87: 1545-1556.
Williams CD, Stengel J, Asike MI, Torres DM, Shaw J, Contreras M, et al. 2011. Prevalence of nonalcoholic fatty liver disease and nonalcoholic steatohepatitis among a largely middle-aged population utilizing ultrasound and liver biopsy: a prospective study. Gastroenterology, 140: 124-131.
Wang F, Pu C, Zhou P, Wang P, Liang D, Wang Q, Hu Y, Li B, Hao X. 2015. Cinnamaldehyde prevents endothelial dysfunction induced by high glucose by activating nrf2. Cell Physiol Biochem, 36: 315-324.
Wang G-W, Deng L-Q, Luo Y-P, Liao Z-H, Chen M. 2016. Hepatoprotective triterpenoids and lignans from the stems of Schisandra pubescens. Nat Prod Res, 16:1855-1860.
Wickenberg J, Lindstedt S, Nilsson J, Hlebowicz J. 2014. Cassia cinnamon does not change the insulin sensitivity or the liver enzymes in subjects with impaired glucose tolerance. Nutr J, 13: 1-6.
Zhao M, Wang P, Zhu Y, Liu X, Hu X, Chen F. 2015. Blueberry anthocyanins extract inhibits acrylamide-induced diverse toxicity in mice by preventing oxidative stress and cytochrome P450 2E1 activation. J Funct Foods, 14: 95-101.
Zhang K, Han ES, Dellinger TH, Lu J, Nam S, Anderson RA, Hu Y, Li B, Hao X. 2016. Cinnamon extract reduces VEGF expression via suppressing HIF‐1α gene expression and inhibits tumor growth in mice. Mol Carcinog, 56: 436-446.
Zheng Y-F, Wei J-H, Fang S-Q, Tang Y-P, Cheng H-B, Wang T-L, Li CY, Peng GP. 2015. Hepatoprotective triterpene saponins from the roots of Glycyrrhiza inflata. Molecules, 20: 6273-6283.