Evaluation of the protective and therapeutic effects of Pistacia atlantica gum aqueous extract on cellular and pathological aspects of experimental asthma in Balb/c mice

Document Type : Original Research Article


1 Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

3 Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

4 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences.

5 Faculty of medicine, Baqiyatallah University of medical sciences, Tehran, Iran


Objective: The purpose of this study was to investigate the protective and therapeutic effects of aqueous extract of P. atlantica gum on an experimental asthma in BALB/c mice.
Materials and Methods: Aqueous extract of dried and milled P. atlantica gum was assemble andevaluate by GC-MS.  In order to investigate the effect of P. atlantica gum extract on cellular and pathological aspects of asthma, 60 BALB/c mice were divided into six groups as: negative control, asthmatic group, asthmatic group receiving dexamethasone (1mg/kg; intraperitoneal (IP)) and three asthmatic groups receiving different concentrations of the extract (100, 200 and 400 mg/kg, orally) from the beginning of the study and continued for 84 days. The examined parameters included cell population, IgE antibody production, levels of IL-4, IL-5, TGF-β, INF-γ, IL-10, and IL-17 cytokines, and lung tissue damage.
Results: Regardless of the dose, aqueous extract of P. atlantica gum, caused significant decrease in the number of BALF eosinophilic cells and levels of anti-ovalbumin IgE, IL-4, IL-5 and IL-17 cytokine levels, as well as pathologic damage of the lung tissue. In addition, the amount of anti-inflammatory IL-10, TGF-β, and INF-γ Th1 cytokines significantly increased in the extract-treated groups compared to the asthmatic and dexamethasone-treated groups. Moreover, IFN-γ/IL-4 ratio significantly increased in a dose-dependent manner compared to the un-treated asthma group.
Conclusion: The aqueous extract of P. atlantica gum can be considered as a potent anti-inflammatory and immunomodulatory compound and may be used as a natural compound for treatment of immune system disorders.


Main Subjects

Abediankenari S, Ghasemi M. 2009. Generation of immune inhibitory dendritic cells and CD4+T regulatory cells inducing by TGF beta. Iran J Allergy Asthma Immunol, 8:25-30.
Abtahi Froushani SM, Esmaili Gourvarchin Galeh H. 2014. New insight into the immunomodulatory mechanisms of Tretinoin in NMRI mice. Iran J Basic Med Sci, 17:632-637.
Abtahi Froushani SM, Esmaili gouvarchin Galee H, Khamisabadi M, Lotfallahzade B. 2015. Immunomudulatory effects of hydroalcoholic extract of Hypericum perforatum. Avicenna J Phytomed, 5: 62-68.
Abtahi Froushani SM, Zarei L, Esmaeili Gouvarchin Ghaleh H, Mansori Motlagh B.2016. Estragole and methyleugenol-free extract of Artemisia dracunculus possess immunomodulatory effects. Avicenna J Phytomed, 6: 526-534.
 Ashokkumar K, Selvaraj K, Muthukrishnan SD. 2013. Cynodon dactylon (L.) Pers.: An updated review of its phytochemistry and pharmacology. J Med Plants Res, 7:3477–3483.
Assaf AM, Abbassi RA, Al-Binni M. 2017.Academic stress-induced changes in Th1- and Th2-cytokine response. Saudi Pharm J, 25:1237-1247.
Bahrami Mahne S, Mahdaviani SA. 2014. Role of the immune cells, mediators and cytokines in the pathogenesis of asthma: a review article. Tehran Univ Med J, 72:273-285.
Chuanfeng F, Xiaoxia L, Hongyan L, Li X, Lei L, Chun Y, Guangqiang G, Xiaofeng J. 2015. Downregulation of SUMF2 gene in the ovalbumin-induced rat model of allergic inflammation. Int J Clin Exp Pathol, 8:12053-12063.
Esmaili Gourvarchin Galeh H, Abtahi Froushani SM, Afzale Ahangaran N, Naji Hadai S. 2018. Effects of educated monocytes with xenogeneic mesenchymal stem cell–derived conditioned medium in a mouse model of chronic asthma. Immunol Invest, 47: 504-520.
Finotto S, Eigenbrod T, Karwot R, Boross I, Doganci A, Ito Nishimoto N, Yoshizaki K, Kishimoto T, Rose-John S, Galle PR, Neurath MF. 2007. Local blockade of IL-6R signaling induces lung CD4+ T cell apoptosis in a murine model of asthma via regulatory T cells. Int Immunol, 19:685-693.
Haghdoost F, Baradaran Mahdavi MM, Zandifari A, Sanei MH, Zolfaghari B, Javanmard SH. 2013. Pistacia atlantica resin has a dose-dependent effect on angiogenesis and skin burn wound healing in the rat. Evid Based Complement Alternat Med, 893425: 1- 8.
Hansbro PM, Kaiko GE, Foster PS. 2011. Cytokine/anti-cytokine therapy- novel treatments for asthma? Br J Pharmacol, 163: 81-95.
Hosseini F, AdlgostarA and Sharifnia F. 2013. Antibacterial activity of Pistacia atlantica extracts on Streptococcus mutans biofilm. Int J Biol Sci, 2:1-7.
Kaliora AC, Stathopoulou MG, Triantafillidis JK, Dedoussis GV, Andrikopoulos NK. 2007. Chios mastic treatment of patients with active Crohn’s disease. World J Gastroenterol, 13: 748-753.
Katzung BG, Masters SB, Trevor AJ. 2012. Basic and clinical pharmacology. 12th ed. New York: McGraw Hill Publications.
Khodaei H, Alizadeh M. 2017. Inhibition of IL-4 but not IFN-γ production by splenocytes of mice immunized with ovalbumin after oral administration of 5-hydroxymethylfurfural. Food Agric Immunol, 28: 27-34.
 Lee SY, Kim JS, Lee JM, Kwon SS, Kim KH, Moon HS, Song JS, Park SH, Kim YK. 2008. Inhaled corticosteroid prevents the thickening of airway smooth muscle in the murine model of chronic asthma. Pulm Pharmacol Ther, 21:9-14.
Levy ML, Hardwell A, McKnight E, Holmes J. 2013. Asthma patients' inability to use a pressurised metered-dose inhaler (pMDI) correctly correlates with poor asthma control as defined by the Global Initiative for Asthma (GINA) strategy: a retrospective analysis. Prim Care Respir J, 22:406-411.
 Lü JM, Lin PH, Yao Q, Chen C. 2010. Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems. J Cell Mol Med, 14:840-860.
Minaiyan M, Karimi F, Ghannadi A. 2015. Anti-inflammatory effect of Pistacia atlantica subsp. kurdica volatile oil and gum on acetic acid-induced acute colitis in the rat.  Int J pharmacogn, 2: 1-12.
Murano N, Murano M, Ishida K, Kawakami K, Abe Y, Inoue T, Morita E, Yasumoto S, Toshina K, Nishikawa T, Ito S, Hirata I, Katsu K. 2006. Therapeutic effect of SHI-219, a novel water soluble prodrug of EG626 (phtalazinol), on mouse dextran sodium sulfate -induced colitis. Bull Osaka Med Coll, 52: 69-79.
 Newcomb DC, Peebles RS. 2013. Th17-mediated inflammation in asthma.  Curr Opin Immunol, 25:10.-27
Nials A,Uddin S. 2008. Mouse models of allergic asthma: acute and chronic allergen challenge, 1: 213-220.
Ober C, Yao TC. 2011. The genetics of asthma and allergic disease: a 21st century perspective. Immunol Rev, 242:10-30.
Rincon M, Irvin CG. 2012. Role of IL-6 in asthma and other inflammatory pulmonary diseases. Int J Biol Sci, 8:1281-1290.
Sharifi MS, Hazell SL. 2011. GC-MS analysis and antimicrobial activity of the essential oil of the trunk exudates from Pistacia atlantica kurdica. J Pharm Sci Res, 3: 1364- 1367.
 Taran M, Mohebali M, Esmaeli J. 2010. In vivo efficacy of gum obtained Pistacia atlantica in the experimental treatment of cutaneous leishmaniasis. Iran J Public Health, 39: 36-41.
Taur DJ, Patil RY. 2011. Some medicinal plants with antiasthmatic potential: a current status. Asian Pac J Trop Biomed, 1:413-418.
Tripathi P, Nair S, Arora N. 2013. Supplementation of antioxidants glutathione and α-lipoic acid attenuates oxidative stress and Th2 response in allergic airway inflammation. Indian J Allergy Asthm, 27:19–26.
Tsai CH, Tung KY, Su MW, Chiang BL, Chew FT, Kuo NW, et al. 2013. Interleukin-13 genetic variants, household carpet use and childhood asthma. PLoS One, 8:e51970.
Zhenghao Li, Jie Zheng. Ning Zhang. 2016. Berberine improves airway inflammation and inhibits NF-κB signaling pathway in an ovalbumin-induced rat model of asthma, J Asthma, 53:999-1005.