In vitro and in vivo antifungal activity of Allium hirtifolium and Allium sativum

Document Type : Original Research Article


Department of Microbiology, Yasooj Branch, Islamic Azad University, Yasooj, Iran


Objective: One of the major clinical problems is the resistance of Candida speciestowards most of antifungals. The search for new antifungal drug from plants have helped to overcome this problem. This study evaluated the effects of Allium hirtifolium and Allium sativumextracts on Candida tropicalis both in vitro and in a mouse model of systemic candidiasis.
Materials and Methods: In this study, clinical isolates of Ctropicalis were isolated and identified from immunocompromised patients with recurrent candidiasis. Antifungal susceptibilities assessment and time kill study of aqueous and ethanolic extracts of A. hirtifolium and A. sativumextracts were done against Ctropicalis. The in vivo activity of aqueous extracts of A. hirtifolium and A. sativumwere evaluated in a mouse model of systemiccandidiasis caused by Ctropicalis through estimating the host survival time, fungal burden and histopathologic analyses.
Results: The aqueous and ethanolic extracts of A. hirtifolium and A. sativum exhibited significant antifungal activity against Ctropicalis. In time kill study, A. hirtifolium and A. sativum extracts exhibited significant effects against Ctropicalis (p<0.05). Treatment of BALB/c mice that were systemicallyinfected with C. tropicalis, showed that treatments with A. hirtifolium and A. sativum (at 1 mg/kg/day) were slightly less efficacious than that of fluconazole in terms of the fungal burden reduction and host survival time, it was still effective against C. tropicalis.
Conclusion: These findings demonstrate the anticandidal properties of A. hirtifolium and A. sativumextracts in vitro and in vivo and suggest their potential to be used as an adjuvant therapy in the management of Candida infections.


Main Subjects

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