Avicenna Journal of Phytomedicine

Avicenna Journal of Phytomedicine

A cytotoxicity and comparative antibacterial study on the effect of Zataria multiflora Boiss, Trachyspermum copticum essential oils, and Enrofloxacin on Aeromonas hydrophila

Document Type : Original Research Article

Authors
Hassan Malekinejad 1
Behnaz Bazargani-Gilani 1
Amir Tukmechi 2
Hadi Ebrahimi 1
1 Department of Pharmacology & Toxicology, Faculty of Veterinary Medicine, Urmia University, Urmia, I. R. Iran
2 Department of Pathobiology & Quality Control, Artemia & Aquatic Animal Research Institute, Urmia University, Urmia, I. R. Iran
10.22038/ajp.2012.108
Abstract
Objective: this study designed to test the antibacterial potency of enrofloxacin (ENR) and essential oils from Zataria multiflora Boiss (ZEO) and Trachyspermum copticum (TEO)on Aeromonas hydrophila.
Material and Methods: The antibacterial potency of test compounds was determined by several methods including the inhibition zone diameter determination, microbroth dilution method and colorimetric method of MTT. The cytotoxicity of test substances was assessed on Chinook salmon (Oncorhynchus tshawytscha) embryo (CHSE-214) cells.
Results: Results showed that ENR and tested essential oils exert antibacterial effect against A. hydrophila. Moreover, ENR exerted the most potent antibacterial effect with MIC values of 62.5 ng/ml. The natural compounds of ZEO and TEO also showed antibacterial effects with rather high MIC values of 0.315 mg/ml, and 1.25 mg/ml, respectively. None of the tested substances showed toxicity on CHSE-24 cells.
Conclusion: It is concluded that ZEO and TEO could be applied to prevent from A. hydrophila infection. Moreover, data also suggest that MTT method could be both cost- and time-effective and accurate method of MIC determination.
Keywords
Natural Antibacterial Substances
Microbroth dilution Method
MTT Technique
Cytotoxicity
Avicenna Journal of Phytomedicine
Volume 2, Issue 4 - Serial Number 4
September and October 2012
Pages 188-195