Antiulcer effects of Zataria multiflora Boiss. on indomethacin-induced gastric ulcer in rats

Document Type : Short communication


1 Department of Pharmacology and Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

3 School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran


Objective: Zataria multiflora has been reported to have several medicinal properties including antioxidant, antibacterial, antispasmodic, and expectorant activities. This study aimed to investigate the effect of Z. multiflora hydro-alcoholic extract (ZMHE) on peptic ulcer caused by indomethacin in rats.
Materials and Methods: ZMHE was prepared by maceration,  condensed by rotary evaporator and dried by a freeze-drier. In this study, 72 male Wistar rats were randomly divided into 12 groups, six in each including: normal rats, control rats, ranitidine-treated, and animals that were treated with ZMHE (100, 200, 400 mg/kg). Parenteral and oral treatments were done 1 and 2 hours, respectively before gastric ulcer induction by indomethacin (25 mg/kg, i.p.). After 6 hours, animals were sacrificed, two sides of the stomach were ligated and gastric contents and tissues were investigated in terms of pH and biochemical and histologic parameters, respectively.
Results: ZMHE (at all doses) considerably decreased the ulcer area and ulcer severity in comparison to control group after oral and parenteral administration. Oral administration of this extract increased thepH of stomach contents while diminished pepsin activity in a dose-dependent manner. Following parenteral treatment, a significant difference in pH of stomach content was observed only by ZMHE 400mg/kg in comparison to control group. The myeloperoxidase (MPO) enzyme activity decreased in groups that received the extract via both oral and parenteral routes.
Conclusion: It might be concluded that ZMHE could protect against experimental gastric ulcer induced by indomethacin and this action is probably mediated via reducing acid secretion and pepsin activity besides enhancing tissue antioxidant capacity


Main Subjects

Abed A, Minaiyan M, Ghannadi A, Mahzouni P, Babavalian MR. 2012. Effect of Echium amoenum Fisch. ET. Mey a traditional Iranian herbal remedy in an experimental model of acute pancreatitis. ISRN Gastroenterol, doi: 10.5402/2012/141548.
Borra SK, Lagisetty RK, Mallela GR. 2011. Anti-ulcer effect of Aloe vera in non-steroidal anti-inflammatory drug induced peptic ulcers in rats. Afr J Pharm Pharmaco, 5:1867-1871.
Boskabady MH, Gholami Mohtaj L. 2014. Effect of the Zataria multiflora on systemic inflammation of experimental animals' model of COPD. Bio Mmed Res Int, doi: 10.1155/2014/802189.
Boskabady MH, Alitaneh S, Alavinezhad A. Carum copticum L. 2014: An herbal medicine with various pharmacological effects. Bio Med Res Int.
Furst DE, Ulrich RW, Prakash SH. 2011. NSAIDs, disease modifying antirrhumatic drugs, nonopioid analgesics and drugs used in gout. In: Katzung BG (Ed.), Basic and Clinical Pharmacology, pp. 637-638, New York, Mc Grow Hill.
Gharib-Naseri M, Mazlomi H, Goshaiesh M, Vakilzadeh G, Heidari A. 2010. Antispasmodic effect of Zataria multiflora Boiss. Leaf extract on the rat uterus. Iran J Pharm Res, 5:131-136
Ghasemi-Dehkordi N. Iranian Herbal Pharmacopoeia. 2002. Ministry of Health Pub. Tehran, 1:105.
Hamauzu Y, Irie M, Kondo M, Fujita T. 2008. Antiulcerative properties of crude polyphenols and juice of apple, and Chinese quince extracts. Food Chem, 108:488-495.
Hosseininejad Z, Moghadam S, Ebrahimi F, Abdollahi M, Zahedi M, Nazari M, et al. 2011. In vitro screening of selected Iranian medicinal plants against Helicobacter pylori. Int J Green Pharm, 5:282-285.
Khazdair MR, Ghorani V, Alavinezhad A, Boskabady MH. 2018. Pharmacological effects of Zataria multiflora Boiss L. and its constituents focus on their anti-inflammatory, antioxidant, and immunomodulatory effects. Fundam Clin Pharmacol, 32:26-50.
Longo DL, Fauci AS, Kasper DL, Hauser SL, Jameson JL, Loscalzo J. 2012. Harrison's Principles of Internal Medicine. In: Valle JD (Ed.), Peptic ulcer disease and related disorders. pp. 683-688, New York, Mc Graw Hill.
Mashak Z, Moradi B. 2012. The combined effect of Zataria multiflora Boiss. And Cinnamomum zeylanicum Nees. Essential oil on the growth of Bacillus cereus in a food model system. J Med Plants, 2:62-73.
Minaiyan M, Ghannadi A, Salehi E. 2005. Antiulcerogenic effect of Zataria multiflora Boiss. On cysteamine induced duodenal ulcer in rats. Iran J Pharm Sci, 1:223-229.
 Minaiyan M, Ghannadi A, Karimzadeh A. 2006. Anti-ulcerogenic effect of ginger (rhizome of Zingiber officinale Roscoe) on cysteamine-induced duodenal ulcer in rats. DARU J. Pharm Sci, 14:97-101.
Minaiyan M, Mahzouni P, Ansari-Roknabady M. 2011a. Effect of Matricaria aurea (Loefl.) Shultz-Bip. hydroalcoholic extract on acetic acid-induced acute colitis in rats. Iran J Basic Med Sci, 14:67-74.
Minaiyan M, Ghannadi A, Mahzouni P, Jaffari-Shirazi E. 2011b. Comparative study of Berberis vulgaris fruit extract and berberine chloride effects on acetic acid-induced colitis in rats. Iran J Pharm Res, 10:97-104.
Motaghi E, Hajhashemi V, Mahzouni P, Minaiyan M. 2016. The effect of memantine on trinitrobenzene sulfonic acid-induced ulcerative colitis in mice. Eur J Pharmacol, 793:28-34.
Nakhai LA, Mohammadirad A, Yasa N, Minaie B, Nikfar S, Ghazanfari G, et al. 2007. Benefits of Zataria multiflora Boiss in experimental model of mouse inflammatory bowel disease. Evid Based Complement Alternat Med, 4:43-50.
Parvan M, Sajjadi S, Minaiyan M. 2017. Protective effect of two extracts of Cydonia oblonga Miller (quince) fruits on gastric ulcer induced by indomethacin in rats. Int J Prev Med, Doi: 10.4103.
Raghuveer B, Chakarvarthy K. 2013. Evaluation of preventive effect of Withania somnifera root extract against ethanol induced ulcers in rats. Int J Bioassays, 2:938-941.
Saei-Dehkordi SS, Tajik H, Moradi M, Khalighi-Sigaroodi F. 2010. Chemical composition of essential oils in Zataria multiflora Boiss. From different parts of Iran and their radical scavenging and antimicrobial activity. Food Chem Toxicol, 48:1562-1567.
Samarghandian S, Azimini-Nezhad M, Farkhondeh T. 2016. The effects of Zataria multiflora on blood glucose, lipid profile and oxidative stress parameters in adult mice during exposure to bisphenol A. Cardiovasc Hematol Disord Drug Targets, 16:41-46.
Shahrani M, Rafieyan M, Pilevarian A, Shirzad H, Hashemzade M, Yousefi H,  et al. 2007. The effect of Amirkabiria odoratissima M. extract on gastric acid and pepsin secretion level in rat. J Shahrekord Univ Med Sci, 8:88-95
Sharafati-Chaleshtori R, Rafieian-Kopaei M, Rokni N, Mortezaei S, Sharafati-Chaleshtori A. 2013.  Antioxidant activity of Zataria multiflora hydroalcoholic extract and its antibacterial effect on Staphylococcus aureus. J Mazandaran Univ Med Sci, 22:88-94.
Sharififar F, Moshafi M, Mansouri S. 2007. In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control, 18:800–805.
Soll AH, Weinstein WM, Kurata J, McCarthy D. 1991. Nonsteroidal anti-inflammatory drugs and peptic ulcer disease. Ann Intern Med, 114:307-19.
Suleyman H, Albayrak A, Bilici M, Cadirci E, Halici Z. 2010. Different mechanisms in formation and prevention of indomethacin-induced gastric ulcers. Inflammation, 33:224-234.
Sung J, Kuipers E, El‐Serag H. 2009. Systematic review: the global incidence and prevalence of peptic ulcer disease. Aliment Pharm Ther, 29:938-946.
Waterman PG, Mole S. 1994. Analysis of Phenolic Plant Metabolites; Ecological Methods and Concepts, pp. 143-154, New York, Wiley & Sons.
Yoshikawa T, Naito Y, Kishi A, Tomii T, Kaneko T, Iinuma S. 1993. Role of active oxygen, lipid peroxidation, and antioxidants in the pathogenesis of gastric mucosal injury induced by indomethacin in rats. Gut, 34:732-737.
Zomorodian K,  Saharkhiz M J,  Rahimi M J,  Bandegi A, Shekarkhar G, Bandegani A, et al. 2011. Chemical composition and antimicrobial activities of the essential oils from three ecotypes of Zataria multiflora. Pharmacogn Mag, 7: 53–59.