Evaluation of antioxidant activity of Malus domestica fruit extract from Kashan area

Document Type : Original Research Article


1 Faculty of Agriculture and Natural Sources, Science and Research Branch, Islamic Azad University, Tehran, I. R. Iran

2 Essential Oils Research Institute, University of Kashan, Kashan, I. R. Iran

3 Isfahan Research Center of Natural Resources, Kashan Station, Kashan, I. R. Iran


Objective: Antioxidants are considered as the main factors in the inhibition of unwanted oxidation reactions.
Materials and Methods: In this research the antioxidant potential of the fresh fruits of 4 cultivars (A to D) of Malus domestica (M. domestica) cultivated in the Kashan, Qamsar area was evaluated. The antioxidant activity of the samples were evaluated using two complementary antioxidant assays: 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene/linoleic acid tests and the results were compared with the synthetic standard antioxidant butylated hydroxytoluene (BHT).
Results: Total phenolic contents of the samples are also estimated by Folin-Ciocalteu's phenol test. In both DPPH β-carotene/linoleic acid tests in the concentration of 2 mg/ml, only samples from cultivar A showed moderate antioxidant activity with 63.92±0.42 and 6.02±0.03 inhibition percentages, respectively and other samples were weakly active.
Conclusion: The Folin-Ciocalteu's phenol test was also showed very little phenolic compounds for the fruits. In conclusion, weak antioxidant activity was estimated for the studied apple cultivars.