Evaluation of antioxidant activity of Malus domestica fruit extract from Kashan area

Jelodarian, Sara; Haghir Ebrahimabadi, Abdolrasoul; Khalighi, Ahmad; Batooli, Hossain

doi:10.22038/ajp.2012.100

Evaluation of antioxidant activity of Malus domestica fruit extract from Kashan area

Document Type : Original Research Article

Authors

Sara Jelodarian ¹

Abdolrasoul Haghir Ebrahimabadi ²

Ahmad Khalighi ¹

Hossain Batooli ³

¹ Faculty of Agriculture and Natural Sources, Science and Research Branch, Islamic Azad University, Tehran, I. R. Iran

² Essential Oils Research Institute, University of Kashan, Kashan, I. R. Iran

³ Isfahan Research Center of Natural Resources, Kashan Station, Kashan, I. R. Iran

10.22038/ajp.2012.100

Abstract

Objective: Antioxidants are considered as the main factors in the inhibition of unwanted oxidation reactions.
Materials and Methods: In this research the antioxidant potential of the fresh fruits of 4 cultivars (A to D) of Malus domestica (M. domestica) cultivated in the Kashan, Qamsar area was evaluated. The antioxidant activity of the samples were evaluated using two complementary antioxidant assays: 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene/linoleic acid tests and the results were compared with the synthetic standard antioxidant butylated hydroxytoluene (BHT).
Results: Total phenolic contents of the samples are also estimated by Folin-Ciocalteu's phenol test. In both DPPH β-carotene/linoleic acid tests in the concentration of 2 mg/ml, only samples from cultivar A showed moderate antioxidant activity with 63.92±0.42 and 6.02±0.03 inhibition percentages, respectively and other samples were weakly active.
Conclusion: The Folin-Ciocalteu's phenol test was also showed very little phenolic compounds for the fruits. In conclusion, weak antioxidant activity was estimated for the studied apple cultivars.

Keywords

Apple

Malus domestica

Extract

Antioxidant activity

Total phenolic content