Evaluation of fibrinolytic and antioxidant effects of Allium elburzense bulb extracts

Document Type: Original Research Article


1 Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

3 Isfahan Center of Public Health Training and Research, Institute of Public Health Research, Tehran University of Medical Science, Iran


Objective: Allium elburzense is an endemic plant of the family Amaryllidaceae that grows wild in northern Iran with some nutritional and medicinal applications. The present study was aimed to investigate the fibrinolytic and antioxidant effects of A. elburzense bulb extracts.
Materials and Methods: Hydroalcoholic, aqueous, chloroformic and butanolic extracts were evaluated in this research. In vitro antioxidant assays were performed using total phenolic, DPPH, and FRAP methods. In the in vivo analysis, animals receivedi.p. injection of A. elburzense hydroalcoholic extract for 21 days and hydroperoxides level, FRAP value, PT and aPTT were determined in serum samples. The fibrinolytic activity of different extracts was quantitatively evaluated by measurement of clot weight.
Results: In vitro antioxidant assay showed that A. elburzense aqueous extract had the highest DPPH scavenging and the highest total antioxidant capacity. In the in vivo assay, A. elburzense hydroalcoholic extract reduced serum hydroperoxides level and increased serum total antioxidant capacity in rats.In vitro fibrinolytic assay revealed remarkable thrombolytic activity for this plant with the highest effect for the aqueous extract. However, coagulation parameters including PT and aPTT were not affected by administration of A. elburzense hydroalcoholic extract in rats.
Conclusion: In conclusion, the results of this study revealed the potential antioxidant and fibrinolytic effects of A. elburzense bulbextracts. For developing novel thrombolytic agents, further investigations for isolation of bioactive constituents and finding the underlying mechanisms are suggested.



Main Subjects

Ackermann RT, Mulrow CD, Ramirez G, Gardner CD, Morbidoni L, Lawrence VA. 2001. Garlic shows promise for improving some cardiovascular risk factors. Arch Intern Med, 161: 813-824.

Ali R, Hossain M, Runa JF, Hasanuzzaman M, Islam M. 2014. Evaluation of thrombolytic potential of three medicinal plants available in Bangladesh, as a potent source of thrombolytic compounds. Avicenna J Phytomed, 4: 430-436.

Anderson FA Jr, Spencer FA. 2003. Risk factors for venous thromboembolism. Circulation, 107: 9-16.

Babaee F, Safaeian L, Zolfaghari B, Haghjoo Javanmard S. 2016. Cytoprotective effect of hydroalcoholic extract of Pinus eldarica bark against H2O2-induced oxidative stress in human endothelial cells. Iran Biomed J, 20: 161-167.

Barile E, Zolfaghari B, Sajjadi SE, Lanzotti V. 2004. Saponins of Allium elburzense. J Nat Prod, 67: 2037-2042.

Barile E, Capasso R, Izzo A, Lanzotti V, Sajjadi SE, Zolfaghari B. 2005. Structure-activity relationships for saponins from Allium hirtifolium and Allium elburzense and their antispasmodic activity. Planta Med, 71: 1010-1018.

Benzie IF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem, 239: 70-76.

Borek C. 2001. Antioxidant Health Effects of Aged Garlic Extract. J Nutr, 131: 1010-1015.

Dacie JV, Lewis SM. 1996. Tests for acute phase response. In: practical haematology, 8th ed., New York, Churchill Livingstone. pp. 559-563.

El-Sabban F. 2009. Garlic as an antithrombotic and antiplatelet aggregation agent. J Chin Clin Med, 4: 288-294.

Everette JD, Bryant QM, Green AM, Abbey YA, Wangila GW, Walker RB. 2010. Thorough study of reactivity of various compound classes toward the Folin-Ciocalteu reagent. J Agric Food Chem, 58: 8139-8144.

Fugh-Berman A.2000. Herbs and dietary supplements in the prevention and treatment of cardiovascular disease.Prev Cardiol, 3: 24-32.

Ghadirkhomi A, Safaeian L, Zolfaghari B, Agha Ghazvini MR, Rezaei P. 2016. Evaluation of acute and sub-acute toxicity of Pinus eldarica bark extract in Wistar rats. Avicenna J Phytomed, 6: 558-566.

Gorinstein S, Drzewiecki J, Leontowicz H, Leontowicz M, Najman K, Jastrzebski Z, et al. 2005.Comparison of the bioactive compounds and antioxidant potentials of fresh and cooked Polish, Ukrainian and Israeli garlic. J Agric Food Chem. 53: 2726-2732.

Hiyasat B, Sabha D, Grotzinger K. 2009. Antiplatelet activity of Allium ursinum and Allium sativum. Pharmacology 83: 197-204.

Iwalewa EO, Adewale IO, Taiwo BJ. 2008. Effects of Harungana madagascariensis stem bark extract on the antioxidantmarkers in alloxan induced diabetic and carrageenan inducedinflammatory disorders in rats. J Complement Integr Med, 5: 1-2.

Kesieme E, Kesieme C, Jebbin N, Irekpita E, Dongo A. 2011. Deep vein thrombosis: a clinical review. J Blood Med, 2: 59-69.

Kim TH, Lee KM, Hong ND, Jung YS. 2015. Anti-platelet and anti-thrombotic effect of a traditional herbal medicine Kyung-Ok-Ko. J Ethnopharmacol, 3: 172-179.

Kimura Y, Okuda H, Ogita Z. 1997. Effects of flavonoids isolated from Scutellariae radix on fibrinolytic system induced by trypsin in human umbilical vein endothelial cells.J Nat Prod, 60: 598–601.

Kini RD, Tripathi Y, Raghuveer CV, Pai SR, Ramaswamy C, Kamath P. 2011. Role of vitamin c as an antioxidant in cadmium chloride induced testicular damage.Int J Appl Biol Pharm Technol, 2: 484-488.

Lanzotti V. 2006. The analysis of onion and garlic. J Chromatogr A, 1112: 3-22.

Lister CE, Hedges LJ. 2007. The nutritional attributes of Allium species, 1814: 1-44.

Matsuura H. 2001. Saponins in garlic as modifiers of the risk of cardiovascular disease. J Nutr, 131: 1000-1005.

Neil A, Silagy C. 1994. Garlic: Its cardioprotective properties. Curr Opin Lipidol, 5: 6-10.

Neshati F, Fritsch RM. 2009. Seed characters and testa sculptures of some Iranian Allium L. species (Alliaceae). Feddes Repert, 120: 322-332.

Onoja SO, Omeh YN, Ezeja MI, Chukwu MN. 2014. Evaluation of the in vitro and in vivo antioxidant potentials of Aframomum melegueta methanolic seed extract. J Trop Med, 2014: 159343.

Rajput MS, Mathur V, Agrawal P, Chandrawanshi HK, Pilaniya U. 2011. Fibrinolytic activity of kaempferol isolated from the fruits of Lagenaria siceraria (Molina) Standley. Nat Prod Res, 25: 1870-1875.

Sikka P, Bindra VK. 2010.Newer antithrombotic drugs. Indian J Crit Care Med, 14: 188–195.

Srivastava KC., Tayagi OD. 1993. Effects of a garlic derived principle (Ajoene) on aggregation and arachidonic acid metabolism in human blood platelets. Prostaglandins Leukot Essent Fatty Acids, 49: 587-595.

Wolf SP. 1994. Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Methods Enzymol, 233: 182-189.

Yoshikawa K, Arihara S, Wang JD, Narui T, Okuyama T. 1991. Structures of two new fibrinolytic saponins from the seed of Luffa cylindrica Roem. Chem Pharm Bull (Tokyo), 39: 1185-1188.

Zhang R, Huang B, Du D, Guo X, Xin G, Xing Z et al. 2013. Anti-thrombosis effect of diosgenyl saponins in vitro and in vivo. Steroids, 78: 1064-1070.

Zolfaghari B,Shokoohinia Y, Ramezanlou P, Sadeghi A, Mahmoudzadeh M, Minaiyan M. 2012. Effects of methanolic and butanolic fractions of Allium elburzense Wendelbo bulbs on blood glucose level of normal and STZ-induced diabetic rats. Res Pharm Sci, 7: 201–207.

Zolfaghari B, Kazemi M, Nematbakhsh M. 2015. The effects of unripe grape extract on systemic blood pressure and serum levels of superoxide dismutase, malondialdehyde and nitric oxide in rat. Adv Biomed Res, 4: 109.