1Faculty of Sciences and Tecniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, Morocco
2ISPITS Rabat, Avenue Hassan II Km 4,5 Route de Casa, Rabat Maroc
32UMR 5018 CNRS-UPS and IFR 31, Rangueil Hospital, L1 Bldg, BP 84225 Toulouse 31432 Cedex 4, France
Objective: As the aqueous extract of Capparis spinosa (CS) possess antidiabetic effect, he present study aims to reveal the possible mechanism of action of CS in diabetic mice.
Materials and Methods: Both single and repeated oral administrations of aqueous extract of CS were performed in multi-low dose streptozotocin-induced (MLDS) diabetic mice. Euglycemic hyperinsulinemic clamp was used in association with the endogenous glucose production (perfusion rate of 3-3H glucose) to evaluate the effect of CS aqueous extract on insulin sensitivity.
Results: Our study showed that aqueous extract of CS possess a potent hypoglycaemic activity in MLDS diabetic mice. Furthermore, the analysis perfusion of 3-3H glucose demonstrated the parallel decrease of basal endogenous glucose production (EGP) with the hypoglycaemic activity. EGP was lower in CS-Treated group when compared to the control group (p<0.001). The euglycemic hyperinsulinemic clamp technique demonstrated that CS treatment improves insulin sensitivity in peripheral tissues.
Conclusion: We conclude that the antihyperglycemic effet CS is probably due to the inhibition of basal endogenous glucose production and the improvement of insulin sensitivity in MLDS diabetic mice.
Adeneye AA. 2012. The leaf and seed aqueous extract of Phyllanthus amarus improves insulin resistance diabetes in experimental animal studies. J Ethnopharmacol, 144: 705-711.
American Diabetes Association. Diagnosis and Classification of Diabetes Mellitus. 2014. Diabetes Care, 37: 81-90.
Burcelin R, Eddouks M, Maury J, Kand J, Assan R, Girard J. 1995. Excessive glucose production, rather than insulin resistance, accounts for hyperglycaemia in recent-onset streptozotocin-diabetic rats. Diabetologia, 38: 283-290.
Eddouks M, Maghrani M, Lemhadri A, Ouahidi ML, Jouad H. 2002. Ethnopharmacological survey of medicinal plants used for the treatment of diabetes mellitus, hypertension and cardiac diseases in the south-east region of Morocco (Tafilalet). J Ethnopharmacol, 82: 97-103.
Hu X, Sato J, Bajjoto G, Khookhor O, et al. 2010. Goshajinkigan improves insulin resistance in diabetic rats via nitric oxide pathway. Nag J Med Sci, 72: 35-42.
Huseini HF, Hasani-Rnjbar S, Nayebi N, et al. 2013. Capparis spinosa L. (Caper) fruit extract in treatment of type 2 diabetic patients: a randomized double-blind placebo-controlled clinical trial. Comp Ther Med, 21: 447-452.
Rahmani R, Mahmoodi M, Karimi M, Hoseini F, Heydari R, Salehi M, Yousefi A. 2013. Effect of Hydroalcoholic Extract of Capparis Spinosa Fruit on Blood Sugar and Lipid Profile of Diabetic and normal Rats. Zahedan J Res Med Sci, 15: 34-38.
Thorvaldson L, Holstad M, Sandler S. 2003. Cytokine release by murine spleen cells following multiple low dose streptozotocin-induced diabetes and treatment with a TNF-alpha transcriptional inhibitor. Inter J Immunopharmacol, 3: 1609-1617.
Tominaga M, Kimura, M, Sygiyama K, et al. 1995. Effects of Sheishin-renshi-in and Gymnema sylvestre on insulin resistance in streptozotocin-induced diabetic rats. Diabetes Res and Clin Pract, 29: 11-17.
Zhou H, Jian R, Kang J, et al. 2010. Anti-inflammatory effects of Caper (Capparis spinosa L.) fruit aqueous extract and the isolation of main phytochemicals. J Agr F Chem, 58: 12717-12721.