@article { author = {Murugan, Sasikumar and Purusothaman, Divya and Richard, Edwin Jothie and Chalichem, Nehru Sai Suresh and Bethapudi, Bharathi and Chandrasekaran, Prasanna Raja and Chinampudur Velusami, Chandrasekaran and D’Souza, Prashanth and Mundkinajeddu, Deepak}, title = {Anti-diarrhoeal activity of a polyherbal formulation in rats and elucidation of its cellular mechanisms}, journal = {Avicenna Journal of Phytomedicine}, volume = {10}, number = {4}, pages = {417-427}, year = {2020}, publisher = {Mashhad University of Medical Sciences}, issn = {2228-7930}, eissn = {2228-7949}, doi = {10.22038/ajp.2019.14339}, abstract = {Objective: The present study was aimed to study anti-diarrhoeal activity of a polyherbal formulation (PHF) in rats and elucidate its mechanism of action. Materials and Methods: Anti-diarrhoeal activity of PHF was investigated using castor oil-induced diarrhoea, small intestinal transit and enteropooling models in rats. PHF was tested at 75, 150 and 300 mg/kg rat body weight. Loperamide was used as a reference control for in vivo studies. Anti-secretory action was evaluated against heat labile enterotoxin (from Escherichia coli)induced secretion in rat ileal loop model. The effect of PHF (12.5-100 µg/ml) on cAMP-dependent secretory activity was investigated against forskolin-induced cAMP release in HT-29 cells. Results: PHF demonstrated significant (p≤0.05) anti-diarrhoeal activity by increasing the time for first faecal drop and inhibited diarrhoeal episodes by 43, 58 and 60% at 75, 150 and 300 mg/kg body weight, respectively in a dose-dependent manner. Also, the intestinal transit was inhibited upto 33% and the weight of secretory contents induced by castor oil was significantly reduced by PHF, approximately 29% in enteropooling assay. On the other hand, the intestinal loop instilled with PHF and enterotoxin from E. coli demonstrated 61% inhibition of fluid accumulation as compared to loop instilled with enterotoxin only. In vitro studies indicated that PHF inhibits cAMP release in HT-29 cells corroborating the anti-secretory effects observed in aforesaid studies. Conclusion: The results suggest that the PHF possesses anti-diarrhoeal activity, evident through reduced faecal output, decreased intestinal transit and anti-secretory activities.}, keywords = {Anti-diarrhoea,Anti-secretory,Castor oil,cAMP,Diarrhoeal drop,Enterotoxin}, url = {https://ajp.mums.ac.ir/article_14339.html}, eprint = {https://ajp.mums.ac.ir/article_14339_29f5fdb3da81e74312f3e85cb8743bcd.pdf} }