Research and Development centre, Natural Remedies Pvt Ltd, Plot No. 5B, Veerasandra Indl. Area, 19th K.M. Stone, Hosur Road, Bangalore 560100, Karnataka, India
Objective: The present study was aimed to study anti-diarrhoeal activity of a polyherbal formulation (PHF) in rats and elucidate its mechanism of action. Materials and Methods: Anti-diarrhoeal activity of PHF was investigated using castor oil-induced diarrhoea, small intestinal transit and enteropooling models in rats. PHF was tested at 75, 150 and 300 mg/kg rat body weight. Loperamide was used as a reference control for in vivo studies. Anti-secretory action was evaluated against heat labile enterotoxin (from Escherichia coli)induced secretion in rat ileal loop model. The effect of PHF (12.5-100 µg/ml) on cAMP-dependent secretory activity was investigated against forskolin-induced cAMP release in HT-29 cells. Results: PHF demonstrated significant (p≤0.05) anti-diarrhoeal activity by increasing the time for first faecal drop and inhibited diarrhoeal episodes by 43, 58 and 60% at 75, 150 and 300 mg/kg body weight, respectively in a dose-dependent manner. Also, the intestinal transit was inhibited upto 33% and the weight of secretory contents induced by castor oil was significantly reduced by PHF, approximately 29% in enteropooling assay. On the other hand, the intestinal loop instilled with PHF and enterotoxin from E. coli demonstrated 61% inhibition of fluid accumulation as compared to loop instilled with enterotoxin only. In vitro studies indicated that PHF inhibits cAMP release in HT-29 cells corroborating the anti-secretory effects observed in aforesaid studies. Conclusion: The results suggest that the PHF possesses anti-diarrhoeal activity, evident through reduced faecal output, decreased intestinal transit and anti-secretory activities.
Murugan, S., Purusothaman, D., Richard, E. J., Chalichem, N. S. S., Bethapudi, B., Chandrasekaran, P. R., Chinampudur Velusami, C., D’Souza, P., & Mundkinajeddu, D. (2020). Anti-diarrhoeal activity of a polyherbal formulation in rats and elucidation of its cellular mechanisms. Avicenna Journal of Phytomedicine, 10(4), 417-427. doi: 10.22038/ajp.2019.14339
MLA
Sasikumar Murugan; Divya Purusothaman; Edwin Jothie Richard; Nehru Sai Suresh Chalichem; Bharathi Bethapudi; Prasanna Raja Chandrasekaran; Chandrasekaran Chinampudur Velusami; Prashanth D’Souza; Deepak Mundkinajeddu. "Anti-diarrhoeal activity of a polyherbal formulation in rats and elucidation of its cellular mechanisms", Avicenna Journal of Phytomedicine, 10, 4, 2020, 417-427. doi: 10.22038/ajp.2019.14339
HARVARD
Murugan, S., Purusothaman, D., Richard, E. J., Chalichem, N. S. S., Bethapudi, B., Chandrasekaran, P. R., Chinampudur Velusami, C., D’Souza, P., Mundkinajeddu, D. (2020). 'Anti-diarrhoeal activity of a polyherbal formulation in rats and elucidation of its cellular mechanisms', Avicenna Journal of Phytomedicine, 10(4), pp. 417-427. doi: 10.22038/ajp.2019.14339
VANCOUVER
Murugan, S., Purusothaman, D., Richard, E. J., Chalichem, N. S. S., Bethapudi, B., Chandrasekaran, P. R., Chinampudur Velusami, C., D’Souza, P., Mundkinajeddu, D. Anti-diarrhoeal activity of a polyherbal formulation in rats and elucidation of its cellular mechanisms. Avicenna Journal of Phytomedicine, 2020; 10(4): 417-427. doi: 10.22038/ajp.2019.14339
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