Fatty acid composition, antioxidant and antibacterial activities of Adonis wolgensis L. extract

Document Type : Original Research Article

Authors

1 Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, I. R. Iran

2 Department of Organic Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, I. R. Iran

3 Department of Medical Engineering, Maziar University, Noor, I. R. Iran

Abstract

Objectives: The objective of this study was to analyze the fatty acid content, antioxidant, and antibacterial activities of hydro-methanolic extract of Adonis wolgensis L. (A. wolgensis L.) growing wild in north of Iran.
Materials and Methods: Oils of A. wolgensis L. was obtained by means of Soxhlet apparatus from leaves and stems. Methyl esters were derived from the oily mixtures by trans-esterification process and were analyzed by GC/FID and GC/MS systems. Phenolic compounds extraction was done with aqueous methanol (90%). This extract was investigated for antioxidant activity using DPPH radical scavenging and reducing power methods and was also tested against a panel of microorganisms.
Results: Linolenic acid (45.83%) and oleic acid (47.54%) were the most abundant fatty acids in leaves and stems, respectively. Hydro-methanolic extract with the high amount of total phenolics (9.20 ±0.011 mg GAE per dry matter) was the potent antioxidant in the assays. Results obtained from measurements of MIC for extract, indicated that E. coli, S. aureus, and S. enteritidis were the most sensitive microorganisms tested, but no activity was observed against Gram-positive microorganism (B. subtilis).
Conclusion: The results obtained from the present study indicated that the oil of A. wolgensis leaves and stems contained a high source of poly-unsaturated fatty acids (PUFAs). These results also showed that hydro-methanolic extract of this plant contained significant antioxidant and antibacterial activities.
 

Keywords

References

Alves MJ, Ferreira ICFR, Dias J, Teixeira V, Martins A, Pintado M. 2012. A Review on Antimicrobial Activity of Mushroom (Basidiomycetes) Extracts and Isolated Compounds. Planta Med, 78: 1707-1718.
Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 26: 1199-1200.
Clinical and Laboratory Standards Institute. 2005. Performance Standards for Antimicrobial Susceptibility Testing. Fifteenth informational supplement, CLSI Document No. M100-S15. CLSI, Wayne PA.
Das UN. 2000. Beneficial effect(s) of n-3 fatty acids in cardiovascular diseases: But, why and how? Prostaglandins Leukot. Essent. Fatty Acids, 63: 351-362.
Dorman HJD, Peltoketo A, Hiltunen R, Tikkanen MJ. 2003. Characterisation of the antioxidant properties of de-odourised aqueous extracts from selected Lamiaceae herbs. Food Chem, 83: 255-262.
Grundy SM. 1997. What is the desirable ratio of saturated, polyunsaturated, and monounsaturated fatty acids in the diet? Am J Clin Nutr, 66: 988S-990S.
Halliwell B, Gutteridge JMC. 1989. Oxidative stress: adaptation damage repair and death. In: Halliwell B, Gutteridge JMC (Eds), Free radical in biology and medicine. pp. 285-295, Oxford, Clarendon Press.
Herald PJ. Davidson PM. 1983. Antibacterial activity of selected hydroxycinnamic acids.  J Food Sci, 48: 1378-1379.
Jelodarian S, Ebrahimabadi AH, Khalighi A, Batooli H. 2011. Evaluation of antioxidant activity of Malus domestica fruit extract from Kashan area. Avicenna J Phytomed, 2: 139-145.
Komissarenko NF, Korzennikova EP, Yatsyuk VY. 1973. Flavonoids of Adonis wolgensis. Chem Nat Cmpd, 9: 417.
Lewis WH, Elvin-Lewis MP. 1995. Medicinal plants as a sources of new therapeutics. Ann Mo Bot Gar, 82: 16-24.
Miceli N, Trovato A, Dugo P, Cacciola F, Donato P, Marino A, Bellinghieri V, Barbera TML, Guvenc A, Taviano M. 2009. Comparative analysis of flavonoid profile, antioxidant and antimicrobial activity of the berries of Juniperus communis L. var. communis and Juniperus communis L. var. saxatilis Pall. from Turkey. J Agric Food Chem, 57: 6570-6577.
Mustafa RA, Hamid AA, Mohamed S, Bakar FA. 2010. Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants. J Food Sci, 75: 28-35.
Paquat C, Hautfenne A. 1992. International Union of Pure and Applied Chemistry, London, Blackwell Scientific Publications.
Pauli GF, Junior P, Berger S, Matthiesen U. 1993. Alepposides, cardenolide oligoglycosides from Adonis aleppica, J Nat Prod, 56: 67-75.
Pauli GF, Junior P. 1995. Phenolic glycosides from Adonis aleppica, Phytochemistry, 38: 1245-1250.
Quereshi S, Pandey AK, Sandhu SS. 2010. Evaluation of antibacterial activity of different Ganoderma lucidum extracts. J Sci Res, 3: 9-13.
Rechinger KH, 1992. Flora Iranica; no. 171, Rechinger, KH (Ed). pp. 204, Graz.
Sedighinia F, Afshar AS, Soleimanpour S, Zarif R, Asili J, Ghazvini K. 2012. Antibacterial activity of Glycyrrhiza glabra against oral pathogens: an in vitro study. Avicenna J Phytomed, 2: 118-124.
Silva EM, Souza JNS, Rogez H, Rees JF, Larondella Y. 2006. Antioxidant activities and polyphenolic contents of fifteen selected plant species from the Amazonian region. Food Chem, 101: 1012-1018.
Singleton VL, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic, 16: 144-158.
Smania EFA, Delle MF, Yunes RA, Paulert R, Smania AJ. 2007. Antimicrobial activity of methyl australate from Ganoderma australe. Braz. J Pharmacog, 17: 14-16.
Stead D. 1993. The effect of hydroxycinnamic acids on the growth of wine-spoilage lactic acid bacteria. J Appl Bacteriol, 135: 135-141.
Sultana B, Anwar F, Przybylski R. 2007. Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees. Food Chem, 104: 1106-1114.
Sun T, Ho CT. 2005. Antioxidant activities of buckwheat extracts. Food Chem, 90:743-749.
Tavakoli R, Mohadjerani M, Hosseinzadeh R, Tajbakhsh M, Naqinezhad A. 2012a. Essential-Oil and Fatty-Acid Composition, and Antioxidant Activity of Extracts of Ficaria kochii. Chem Biodiversity, 9: 2732-2741.
Tavakoli R, Mohadjerani M, Hosseinzadeh R, Tajbakhsh M, Naqinezhad A. 2012b. Determination of Chemical Composition of Essential Oil from Aerial Parts of Adonis wolgensis Grown in North of Iran by GC-MS. Anal Chem Lett, 2: 125-128.
Tekeli Y, Sezgin M, Aktumsek A, Guler GO, Sanda MA. 2010. Fatty acid composition of six Centaurea species growing in Konya, Turkey. Nat Prod Res, 24: 1883-1889.
Wang WT. 1994. Revision of Adonis (Ranunculaceae) I, Bull Bot Res, 14: 1-31.
Yildirim A, Mavi A, Kara AA. 2001. Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J Agric Food Chem, 49: 4083-4089.
 
Avicenna Journal of Phytomedicine
Volume 4, Issue 1 - Serial Number 1
January and February 2014
Pages 24-30

Files

Share

How to cite

Statistics