Immunomodulatory activity of geranial, geranial acetate, gingerol, and eugenol essential oils: evidence for humoral and cell-mediated responses

Document Type : Original Research Article

Authors

1 Research Scholar, Research and Development, Bharathiar University, Coimbatore, Tamilnadu, India - 641 046

2 Department of Biotechnology, Mohamed Sathak College of Arts and Science, Chennai, India- 119. Sholinganallur, Chennai, Tamil Nadu, India

Abstract

Objective: The immunomodulatory effect of geranial, geranial acetate, gingerol, and eugenol essential oils were evaluated by studying humoral and cell-mediated immune responses.
Materials and Method: The essential oils were evaluated for immunomodulatory activity in in vivo studies, using rats as the animal model. The essential oils were tested for hypersensitivity and hemagglutination reactions, using sheep red blood cells (SRBC) as the antigen while sodium carboxy methyl cellulose (SCMC) served as the control in all the tests.
Result: Orally administrated essential oils showed a significant increase of test parameters, viz., haemagglutinating antibody titre (HAT) and delayed type hypersensitivity (DTH) response. In rats immunized with sheep RBC, essential oils enhanced the humoral antibody response to the antigen and significantly potentiated the cellular immunity by facilitating the foot pad thickness response to sheep RBC in sensitized rats with doses of 50-800 mg/ml. Haemagglutination titre of geraniol showed the highest increase of 139.3±6.38 and with 5.9±0.7 DTH, respectively. For geranial acetate, the haemagglutination titre showed a moderate increase of 87.5±5.9 and highest increase in DTH with 5.9±0.8, respectively. Using gingerol, the haemagglutination titre showed a moderate increase with 88.2±6.306 and DTH 3.5±0.5, respectively and for eugenol, the haemaggulation titre showed a moderate increase with 112.06±6.169 and DTH 4.4±0.6, respectively. These differences were statistically significant.
Conclusion: The essential oils were found to have a significant immunostimulant activity on both the specific and non-specific immune mechanisms.

Keywords

References

Ahmad I, Mehmood Z, Mohammad F. 1998. Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol,  62: 183-193.
Benacerraf B. 1978. A hypothesis to relate the specificity of T lymphocytes and the activity of I region specific Ir genes in macrophages and B-lymphocytes. J Immunol, 120: 1809-1812.   
Berahou A, Auhmani A, Fdil N, Benharref A, Jana M, Gadhi CA. 2007. Antibacterial activity of Quercus ilex bark’s extracts. J Ethnopharmacol,  112: 426-429.
Carrasco FR, Schmidt G, Romero AL, Sartoretto JL, Caparroz-Assef SM, Bersani-Amado CA, Cuman RK. 2009. Immunomodulatory activity of Zingiber officinale Roscoe, Salvia officinalis L. and Syzygium aromaticum L. essential oils: evidence for humor- and cell-mediated responses. J Pharm Pharmacol, 61: 961-967.
Doherty NS. 1981. Selective effect of immunosuppressive agents against the delayed hypersensitive response and humoral response to sheep red blood cells in mice. Agents Actions, 11: 237-242. 
Elgert KD. New York: Wiley; 1996. Immunology: Understanding the immune system; p. 306
Gabhe SY, Tatke PA, Khan TA. 2006. Evaluation of the immunomodulatory activity of the methanol extract of Ficus benghalensis roots in rats. Indian J Pharmacol, 38: 271-275. 
Golab M, Burdzenia O, Majewski P, Skwarlo-Sonta K. 2005. Tea tree oil inhalations modify immunity in mice. J Appl Biomed, 3: 101-108
Harkenthal M, Reichling J, Geiss HK, Saller R.1999. Comparative study on the in vitro antibacterial activity of Australian tea tree oil, cajuput oil, niaouli oil, manuka oil, kanuka oil, and eucalyptus oil. Pharmazie, 54: 460.  
Kabara JJ. 1991. Phenols and chelators. In: N.J. Russell and G.W. Gould, Editors, Food preservatives, Blackie, Glasgow, pp. 200-214.
Nelson DS, Mildenhall P. 1967. Studies on cytophilic antibodies. The production by mice of macropage cytophilic antibodies to sheep erythrocytes: relationship to the production of other antibodies and the development of delayed type hypersensitivity. Aust J Exp Biol and Med Sci, 45: 113-130.
Paget GE, Barnes JM. 1969. Evaluation of drug activities, pharmacometrics. In: Lawranle DR, Bacharch AL, editors. New York: Academic press; 1: 161. 
Raphael TJ, Kuttan G. 2003. Immunomodulatory Activity of Naturally Occurring Monoterpenes  Carvone, Limonene, and Perillic Acid, 25: 285-294.
Reichling J. Plant-microbe interaction and secondary metabolites with antiviral, antibacterial and antifungal properties. In Functions of Plant Secondary Metabolites and Their Exploitation in Biotechnology, Ann Plant Rev —Wink M, ed. 1999 3 Sheffield: Sheffield Academic Press. 187- 273.
 
Avicenna Journal of Phytomedicine
Volume 3, Issue 3 - Serial Number 3
May and June 2013
Pages 224-230

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