TY - JOUR ID - 11788 TI - Protective effect of Berberis vulgaris on Fenton reaction-induced DNA cleavage JO - Avicenna Journal of Phytomedicine JA - AJP LA - en SN - 2228-7930 AU - Asadi, Nooshin Sadat AU - Heidari, Mohammad Mehdi AU - Khatami, Mehri AD - Department of Biology, Faculty of Science, Yazd University, Yazd, Iran Y1 - 2019 PY - 2019 VL - 9 IS - 3 SP - 213 EP - 220 KW - Antioxidant activity KW - Berberis vulgaris KW - DNA damage KW - Fenton reaction DO - 10.22038/ajp.2018.11788 N2 - Objective: Berberis vulgaris contains antioxidants that can inhibit DNA cleavage. The purpose of this study was to evaluate the antioxidant and protective activity of B. vulgaris on DNA cleavage. Materials and Methods: In this study, the antioxidant capacity of B. vulgaris was investigated using DPPH and its protective effect was evaluated on pBR322 plasmid and lymphocyte genomic DNA cleavage induced by Fenton reaction, by DNA electrophoresis. Results: Aqueous extract of B. vulgaris presented dual behavior with a potent antioxidant activity at 0.25and 0.75mg/ml for pBR322 plasmid and lymphocyte genomic DNA, respectively, but a pro-oxidant activity was observed at higher concentrations. Conclusion: Our results indicated that B. vulgaris extract an inhibit Fenton reaction-induced DNA cleavage and oxidative cleavage of double-stranded DNA assay is a powerful technique that can be used to determine the antioxidant and pro-oxidant properties of a compound on cellular components such as DNA. UR - https://ajp.mums.ac.ir/article_11788.html L1 - https://ajp.mums.ac.ir/article_11788_a4f9d93a31be7b2a8495495ea302f212.pdf ER -