Polyphenols isolated from virgin coconut oil attenuate cadmium-induced dyslipidemia and oxidative stress due to their antioxidant properties and potential benefits on cardiovascular risk ratios in rats

Document Type : Original Research Article

Authors

1 Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria

2 Department of Medical Biochemistry, College of Medicine, University of Nigeria, Enugu Campus, Enugu State, Nigeria

Abstract

Objective: Literature has confirmed the pathogenic role of cadmium (Cd) and its exposure in the induction of dyslipidemia implicated in the development and increasing incidence of cardiovascular diseases. The current study explored whether polyphenolics isolated from virgin coconut oil (VCO) prevent Cd-induced dyslipidemia and investigate the underlying mechanism of action, in rats.
Materials and Methods: Rats were pretreated with VCO polyphenols (10, 20 and 50 mg/kg body weight; orally) 2 weeks prior to concurrent Cd administration (5 mg/kg) for 5 weeks. Subsequently, serum concentrations of lipid and lipoprotein cholesterol and cardiovascular risk ratios were determined. Hepatic activities of superoxide dismutase (SOD) and catalase (CAT) as well as reduced glutathione (GSH) and malondialdehyde (MDA) contents were analyzed.
Results: Sub-chronic Cd administration significantly increased the serum levels of total cholesterol, triglycerides, low density lipoprotein cholesterol and very low density lipoprotein cholesterol while markedly reduced high density lipoprotein cholesterol. Hepatic activities of SOD and CAT as well as GSH content were suppressed by Cd, whereas MDA level was obviously increased. The co-administration of VCO polyphenol with Cd remarkably restored lipid profile and cardiovascular risk ratios and stabilized antioxidant defense systems comparable to control group.
Conclusion: This is the first study presenting that polyphenols isolated from VCO prevent Cd-induced lipid abnormalities and cardiovascular risk ratios by improving antioxidant defense systems.

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Main Subjects

References

Abbott RD, Wilson PW, Kannel WB and Castelli WP. 1988. High density lipoprotein cholesterol, total cholesterol screening and myocardial infarction. The Framingham Study. Arteriosclerosis, 8: 207–211
Alladi S, Khada A, Shanmugan M. 1989. Induction of hypercholesterolemia by simple soil protein with acetate generating amino acid. Nutr Rep Int, 40: 893–894.
Arthur JR and Boyne R. 1985. Superoxide dismutase and glutathione peroxidase activities in neutrophils from selenium deficient and copper deficient cattle. Life Sci, 36: 1569-1575
Bashir N, Manoharan V and Prabu SM. 2014. Ameliorative effects of grape seed proanthocyanidins on cadmium-induced metabolic alterations in rats. Int J Biol Res, 2: 28-34.
Belyaeva EA and Korotkov SM. 2003. Mechanism of primary Cd2+-induced rat liver mitochondria dysfunction: discrete modes of Cd2+ action on calcium and thiol-dependent domains. Toxicol Appl Pharmacol, 192: 56–68.
Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 26: 1199-1200.
Byrne C, Divekar SD, Storchan GB, Parodi DA, Martin MB. 2009. Cadmium - A metallohormone? Toxicol Appl Pharmacol, 238: 266–271
Covas M, Nyyssönen K, Poulsen H, Kaikkonen J, Zunft HF, Kiesewetter H, Gaddi A, de la Mursu, TR, Bäumler H, Nascetti S, Salonen JT, Fitó M, Virtanen J, Marrugat J. 2006. The effect of polyphenols in olive oil on heart disease risk factors- A randomized trial. Ann Intern Med, 145: 333-341
Danavi JV, Memon SB and Phan BA. 2015. Statin alternatives: A review of over-the-counter lipid-lowering supplements. Altern Complement Ther, 21: 198-209.
Edwards JR and Prozialeck WC. 2009. Cadmium, diabetes and chronic kidney disease. Toxicol Appl Pharmacol, 238: 289–293.
Exner R, Wessner B, Manhart N and Roth E. 2000. Therapeutic potential of glutathione. Wien Klin. Wochenschr, 112: 610-616.
Fakurazi S, Sharifudin SA, Arulselvan P. 2012. Moringa oleifera hydroethanolic extract effectively alleviates acetaminophen-induced hepatotoxicity in experimental rats through their antioxidant nature. Molecules, 17: 8334-8350.
Famurewa AC, Ufebe OG, Egedigwe CA, Nwankwo OE and Obaje GS. 2017. Virgin coconut oil supplementation attenuates acute chemotherapy hepatotoxicity induced by anticancer drug methotrexate via inhibition of oxidative stress in rats. Biomed Pharmacother, 87: 437-442.
Gasevic D, Frohlich J, Mancini GB, Lear SA. 2014. Clinical usefulness of lipid ratios to identify men and women with metabolic syndrome: A cross-sectional study. Lipids Health Dis, 13: 159.
Grundy SM, Goodman DW, Rifkind BM, Cleeman JI. 1989. The place of high density lipoprotein in cholesterol management: A prospective from the National Cholesterol Education Program. Arch Intern Med, 149(3): 505-10.
Illam SP, Narayanankutty A, and Raghavamenon AC.2017.Polyphenols of virgin coconut oil prevent pro-oxidant mediated cell death. Toxicol Mech Meth, doi: 10.1080/ 15376516 .2017.1320458.
Ingelsson E, Schaefer E, Contois JH, McNamara JR, Sullivan L, Keyes MJ, et al. 2007. Clinical utility of different lipid measures for prediction of coronary heart disease in men and women. JAMA, 298: 776–785.
Larregle EV, Varas SM, Oliveros LB, Martinez LD and Antón R. 2008. Lipid metabolism in liver of rat exposed to cadmium. Food Chem Toxicol, 46: 1786-1792.
Marina AM, Che Man YB, Amin I. 2009. Virgin coconut oil: emerging functional food oil.   Trends Food Sci Technol, 20:481-487.
Mayes PA and Botham KM. 2003. Lipid transport and storage. In: Murray RK, Granner DK, Mayes PA, Rodwell VW (eds). Harper’s illustrated biochemistry. 26th ed. USA: McGraw-Hill Companies Inc; p 205–218.
Millán J, Pintó X, Muñoz A, Zúñiga M, Rubiés-Prat J, Pallardo LF, Masana L,  Mangas A,
     Hernández-Mijares A, González-Santos P, Ascaso JF, Pedro-Botet J. 2009. Lipoprotein ratios: Physiological significance and clinical usefulness in cardiovascular prevention. Vasc Health Risk Manag, 5: 757–765.
Morales AI. 2008. Effect of the flavonoid quercetin on cadmium-induced hepatotoxicity. Food Chem Toxicol, 46; 2279–2287
National Research Council, Guide for the Care and the Use of Laboratory Animals, 20, National Institute of Health, Bethesda (1985) pp. 85–123.
Nevin KG and Rajamohan T. 2004. Beneficial effects of virgin coconut oil on lipid parameters and in vitro LDL oxidation. Clin Biochem, 37: 830–835.
Olisekodiaka MJ, Igbeneghu CA, Onuegbu AJ, Oduru R, Lawal AO. 2012. Lipid, lipoproteins, total antioxidant status and organ changes in rats administered high doses of cadmium chloride. Med Princ Pract, 21: 156–159.
Oyaizu M. 1986. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr, 44: 307-315
Prabu SM, Muthumani M and Shagirtha K. 2013. Quercetin potentially attenuates cadmium induced oxidative stress mediated cardiotoxicity and dyslipidemia in rats. Eur Rev Med Pharma Sci, 17: 582-595.
Prabu SM, Shagirtha K and Renugadevi J. 2010. Amelioration of cadmium-induced oxidative stress, impairment in lipids and plasma lipoproteins by the combined treatment with quercetin and α- tocopherol in rats. J Food Sci, 75: T132–T140
Prozialeck WC and Edwards JR. 2012. Mechanisms of cadmium-induced proximal tubule injury: new insights with implications for biomonitoring and therapeutic interventions. J Pharmacol Exp Ther, 343: 2-12.
Rahim NS, Lim SM, Mani V, Abdul-Majeed A and Ramasamy K. 2017. Enhanced memory in Wistar rats by virgin coconut oil is associated with increased antioxidative, cholinergic activities and reduced oxidative stress. Pharm Biol, 55: 825–832.
Ramirez DC and Gimenez MS. 2002. Lipid modification in mouse peritoneal macrophages after chronic cadmium exposure. Toxicology, 172: 1-12.
Renugadevi J and Prabu SM. 2010. Cadmium-induced hepatotoxicity in rats and the protective   effect of naringenin. Exp Toxicol Pathol, 62: 171–181
Renugadevi J, and Prabu SM. 2009. Naringenin protects against cadmium-induced oxidative renal dysfunction in rats. Toxicology, 256: 128–134
Rogalska J, Brzóska MM, Roszczenko A and Moniuszko-Jakoniuk J. 2009. Enhanced zinc consumption prevents cadmium-induced alterations in lipid metabolism in male rats. Chem Biol Interact, 177: 142–152.
Samarghandian S, Azimi-Nezhad M, Samini F. 2014. Ameliorative effect of saffron aqueous extract on hyperglycemia, hyperlipidemia, and oxidative stress on diabetic encephalopathy in streptozotocin-induced experimental diabetes mellitus. Biomed Res Int, 9: 208–257.
Samarghandian S, Azimi-Nezhad M, Shabestari MM, Azad FJ, Farkhondeh T, Bafandeh F. 2015. Effect of chronic exposure to cadmium on serum lipid, lipoprotein and oxidative stress indices in male rats. Interdiscip Toxicol, 8: 151–154.
Senthilkumar T, Sangeetha N and Ashokkumar N. 2012. Antihyperglycemic, antihyperlipidemic, and renoprotective effects of Chlorella pyrenoidosa in diabetic rats exposed to cadmium. Toxicol Mech Meth, 22: 617–624
Sinha AK. 1972. Colorimetric assay of catalase. Anal Biochem, 47: 389-394
Siu ER, Mruk DD, Porto CS, and Cheng CY. 2009. Cadmium-induced testicular injury. Toxicol Appl Pharmacol, 238: 240–249.
Tangvarasittichai S, Niyomtam S, Pingmuangkaew P, Nunthawarasilp P. 2015. Dyslipidemia in the elevated cadmium exposure population. Int J Toxicol Pharm Res, 7: 92-98.
Tangvarasittichai S, Poonsub P and Tangvarasittichai O. 2010. Association of serum lipoprotein ratios with insulin resistance in type 2 diabetes mellitus. Indian J Med Res, 13: 641-648.
Vega GL, Barlow CE, Grundy SM, Leonard D and DeFina LF. 2014. Triglyceride-to high-density- lipoprotein-cholesterol ratio is an index of heart disease mortality and of incidence of type 2 diabetes mellitus in men. J Investig Med, 62: 345–349
Vicente-Sánchez C, Egido J, Sanchez-Gonzalez PD, Perez-Barriocanal F, Lopez-Novoa JM,Morales AI. 2008. Effect of the flavonoid quercetin on cadmium-induced hepatotoxicity. Food Chem Toxicol, 46: 2279–2287.
Vidal AC, Semenova V, Darrah T, Vengosh A, Huang Z, King K, Nye MD, Fry R, Skaar D, Maguire R, Murtha A, Schildkraut J, Murphy S, Hoyo C. 2015. Maternal cadmium, iron and zinc levels, DNA methylation and birth weight. BMC Pharmacol Toxicol, 16: 20-28.
Vysakh A, Ratheesh M, Rajmohanan TP, Pramod C, Premlal S, Girishkumar B, Sibi PI.  2014. Polyphenolics isolated from virgin coconut oil inhibits adjuvant induced arthritis in rats through antioxidant and anti-inflammatory action. Int Immunopharmacol, 20: 124-130.
Wallin B, Rosengren B, Shetzer HG and Cameja G. 1993. Lipid oxidation and measurement of thiobarbituric acid reacting substances (TBARS) formation in a single microtitre plate: its use for evaluation of antioxidants. Anal Biochem, 208: 10-15.
Wangensteen H, Samuelsen AB and Malterud KE. 2004. Antioxidant activity in extracts from coriander. Food Chem, 88(2), 293–297
Zhao M-J, Wang S-S, Jiang Y, Wang Y, Shen H, Xu P, et al, 2017. Hypolipidemic effect of XH601 on hamsters of hyperlipidemia and its potential mechanism. Lipids Health Dis, 16:85-96.
Zhou Z,  Lu Y,  Pi H, Gao P, Li M, Zhang L, Pei L-P, Mei X, Liu L, Zhao Q, Qin Q-Z, Chen Y, Jiang Y-M, Zhang Z-H, Yu Z-P.  2016. Cadmium exposure is associated with the prevalence of dyslipidemia. Cell Physiol Biochem, 40
Avicenna Journal of Phytomedicine
Volume 8, Issue 1
January and February 2017
Pages 73-84

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