Effects of ethanolic extract of Jasminum grandiflorum Linn. flowers on wound healing in diabetic Wistar albino rats

Document Type : Original Research Article

Authors

1 GMERS Medical College, Junagadh, Gujarat, India

2 Billev Pharma East , Ljubljana, Slovenia

3 Government Medical College, Bhavnagar, Gujarat, India

Abstract

Objective: To evaluate wound healing activity of ethanolic extract of Jasminum grandiflorum Linn. (J. grandiflorum) flowers in diabetic rats.
Materials and Methods: Streptozotocin-induced diabetic Wistar albino rats were divided into six groups (n=6).Three groups – diabetic control, positive control (that received Glibenclamide) and treatment (that received J. grandiflorum Linn. Flower extract) were operated for excision wounds (EW). These groups were evaluated for wound contraction and re-epithelization. The other three groups were operated for incision wounds (IW) and dead space wounds (DW). Incision and dead space wounds were produced in the same rats. IWs were analyzed for wound breaking strength and the granulation tissues from DWs were analyzed for dry weight, hydroxyproline content, and histology.
Results: IWs and DWs showed significant improvement in wound breaking strength (265.8±10.4 vs 332.5±8.2; p<0.05), granulation tissue dry weight (26.1±0.6vs 40.4±0.3; p<0.01) and hydroxyproline content (19.3±0.5 vs 32.6±0.8; p<0.01) in treatment group as compared to control group. Neo-angiogenesis was also high in treatment group. Wound contraction was earlier (day 14) in treatment group compared to diabetic control (day 20). No significant improvement was seen in re-epithelization in treatment group.
Conclusion: Ethanolic extract of J. grandiflorum Linn. flowers increases granulation tissue formation as well as neo-angiogenesis. It also enhances wound contraction; however, re-epithelization was not significantly affected. J. grandiflorum Linn. flowers could be potentially effective in promotion of diabetic wounds healing by increasing granulation tissue formation and enhancing wound contraction; however, further studies are required for its clinical application.
.

Keywords

Main Subjects

References

Abramov Y, Golden B, Sullivan M, Botros SM, Miller J, Alshahrour A, Goldberg  R and Sand P.2007. Histologic characterization of vaginal vs. abdominal surgical wound healing in a rabbit model. Wound Repair Regen, 15: 80-86.
Anderson J. 2003. Nitric oxide, atherosclerosis and the clinical relevance of endothelial dysfunction. Heart Fail Rev, 8: 71-86.
Dipasquale G, Meli A. 1965. Effect of body weight changes on the formation of cotton pellet-induced granuloma. J Pharm Pharmacol, 17: 379-382.
Ehrlich P, Hunt K. 1969. The effects of cortisone and anabolic steroids on the tensile strength of healing wounds. Ann Surg, 170: 203-206.
Falanga V.2005.Wound healing and its impairment in the diabetic foot. Lancet, 366: 1736-1743.
Ghori V, Mandavia R, Patel K, Tripathi CB. 2014. Effect of topical nitric oxide donor (0.2 % glyceryltrinitrate) on wound healing in diabetic Wistar rats. Int J Diab Dev Ctries, 34: 45-49.
Isner M. 2001. Method of using angiotensin converting enzyme inhibitor to stimulate angiogenesis. U.S. Patent No. 6,191,144.
Kleinman K, Malinda M. 2000. Role of angiogenesis in wound healing. In: Mousa SA, Angiogenesis inhibitors and stimulators: potential therapeutic implications. pp. 102-110.
Kolanjiappan K, Manoharan S. 2005. Chemopreventive efficacy and anti-lipid peroxidative potential of Jasminum grandiflorum Linn. on 7,12-dimethylbenz (a) anthracene-induced rat mammary carcinogenesis. FundamClinPharmacol, 19: 687-693.
Lee H. 1968. Studies on the mechanism of action of salicylate. II. Retardation of wound healing by aspirin. J Pharm Sci, 57: 1042-1043.
Luo D, Wang Y, Fu WL, Wu J, Chen F.2004.Gene therapy of endothelial nitric oxide synthase and manganese superoxide dismutase restores delayed wound healing in type 1 diabetic mice. Circulation, 110: 2484–2493.
McMurry JF Jr. 1984. Wound healing with diabetes mellitus. Better glucose control for better wound healing in diabetes. SurgClin North Am, 64: 769-78.
Mishra SB, Mukerjee A, Vijayakumar M. 2010. Wound healing activity of the aqueous alcoholic extract of Jasminum Grandiflorumlinn leaves. Pharmacologyonline, 3: 35-40.
Morton J, Malone H. 1972. Evaluation of vulneray activity by an open wound procedure in rats. Arch IntPharmacodynTher, 196: 117-126.
Nayak S, Krishna M. 2007.Influence of ethanolic extract of Jasminum grandiflorum Linn flower on wound healing activity in rats. Indian J PhysiolPharmacol, 51: 189–194.
PaarakhS,Paarakh P. 2009.Jasminum grandiflorum Linn (Chameli): Ethnobotany, Phytochemistry and Pharmacology – A review. PharmacologyOnline Newslett, 2: 586-595.
Patil M, Saini R. 2012. Anticonvulsant activity of methanolic extract of Jasminum grandiflorum Linn in experimental animals. Res J Pharm BiologChemSci, 3:43-49.
Ramsey SD, Newton K, Blough D, McCulloch DK, Sandhu N, Reiber GE, Wagner E. 1999. Incidence, outcomes, and cost of foot ulcers in patients with diabetes.Diabetes Care, 22: 382-387.
Ramundo J, Gray M.2008. Enzymatic wound debridement.J Wound Ostomy Continence Nurs, 35:273-280.
Schmidt M, Hori O, Brett J, Yan D, Wautier L, Stern D. 1994. Cellular receptors for advanced glycation end products. Implications for induction of oxidant stress and cellular dysfunction in the pathogenesis of vascular lesions.ArteriosclerThromb, 14:1521-1528.
Singer J, Clark A.  1999. Cutaneous Wound Healing. N Engl J Med, 341: 738-746.
SiwikA, Pagano J, Colucci S. 2001. Oxidative stress regulates collagen synthesis and matrix metalloproteinase activity in cardiac fibroblasts. Am J Physiol Cell Physiol, 280: C53–C60.
Somanadhan B, Smitt W, George V, Pushpangadan P, Rajasekharan S, Duus O, Nyman U, Olsen C, and Jaroszewski J. 1998. Angiotensin converting enzyme (ACE) inhibitors from Jasminum azoricum and Jasminum grandiflorum. Planta Medica, 64:246-250.
Sumi Y, Ishihara M, Kishimoto S, Takikawa M, Hattori H, Takikawa M, Azuma R, Nakamura S, Fujita M, and Kiyosawa T. 2014. Effective wound healing in streptozotocin-induced diabetic rats by adipose-derived stromal cell transplantation in plasma-gel containing fragmin/protamine microparticles. Ann PlastSurg, 72: 113-120.
Teixeira S, Caliari V, Rocha A, Machado D, Andrade P. 1999. Aminoguanidine Prevents Impaired Healing and Deficient Angiogenesis in Diabetic Rats. Inflammation, 23: 569-581.
Traub O, Bibber R. 1995. Role of Nitric Oxide in Insulin-Dependent Diabetes mellitus related Vascular Complication. West J Med, 162: 439-445.
Tsuchiya H, Sato M, Miyazaki T, Fujiwara S, Tanigaki S, Ohyama M et al., 1996. Comparative study on the antibacterial activity of phytochemical flavanones against methicillin-resistant Staphylococcus aureus. J Ethnopharmacol, 50:27-34.
Umamaheswari M, Ashokkumar K, Rathidevi R, Sivashanmugam T, Subbhardadevi V, Ravi K.2007.Anti ulcer and in vitro antioxidant activities of Jasminum grandiflorum L. J Ethnopharmacol, 110: 464-470.
Vidyalakshmi A, Selvi E. 2013.Protease Activity of Floral Extracts of Jasminum grandiflorum L., A Wound Healing Herb. J Med Plants Stud, 1: 11-15.
Villegas F, Fernández D, Maldonado H, Torres R, Zavaleta A, Vaisberg J, Hammond G. 1997. Evaluation of the wound-healing activity of selected traditional medicinal plants from Peru. J Ethnopharmacol, 55: 193–200.
Walsh G (Ed).2003. Debriding agents, in Biopharmaceuticals: Biochemistry and Biotechnology, 2nd Ed, pp 398. John Wiley & Sons Ltd. England.
Woessner JFJr. 1961. The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch Biochem Biophys, 93: 440-447.
 
Avicenna Journal of Phytomedicine
Volume 7, Issue 5
September and October 2017
Pages 401-408

Files

Share

How to cite

Statistics