Curcumin attenuates harmful effects of arsenic on neural stem/progenitor cells

Document Type : Original Research Article

Authors

1 Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran

2 Department of Biology, Kharazmi University of Tehran, Iran

3 Department of Neuroscience, Mashhad University of Medical Sciences, Mashhad, Iran

4 Epilepsy Research Center, Department of Neurology, and Department of Neurosurgery, Westfälische Wilhelms-Universität Münster, Münster, Germany

Abstract

Objective: Arsenic, an environmental pollutant, decreases neuronal migration as well as cellular maturation and inhibits the proliferation of neural progenitor cells. Curcumin has been described as an antioxidant and neuroprotective agent with strong therapeutic potential in some neurological disorders. Human adipose-derived stem cells (hADSCs), a source of multipotent stem cells, can self-renew and differentiate into neural cells. The aim of the present study was to investigate the preventive effect of curcumin against arsenic toxic effects on the viability, telomerase activity, and apoptosis of neural stem/progenitor cells (NSPCs) derived from hADSCs.
Materials and Methods: The characteristics of human adipose tissue were identified by immunocytochemistry for surface markers namely, CD105, CD73, and CD90. Using neurosphere assay, hADSCs were differentiated into neuronal cells. To characterize neural cells, expression of nestin, SOX2, MAP2, and GFAP were assessed by immunocytochemistry. Cytotoxicity and viability of NSPCs were evaluated by MTT assay. Reactive oxygen species (ROS) generated by arsenic exposure, were measured and caspase 3/7 activity and caspase-3 processing as well as the telomerase activity were determined.
Results: The isolated hADSCs positively expressed CD105, CD73, and CD90. Nestin, Sox2, GFAP, and MAP2 were expressed in the neurospheres derived from hADSCs. Curcumin/arsenic co-treatment significantly increased telomerase activity of NSPCs compared to arsenic group. Furthermore, curcumin significantly reduced arsenic-induced apoptosis (via inactivation of caspases) as well as arsenic-associated ROS generation.
Conclusion: Our findings revealed that curcumin has the potential to prevent harmful effects of arsenic on neurogenesis.

Keywords

Main Subjects

References

Andersen HR, Nielsen JB, Grandjean P. 2000. Toxicologic evidence of developmental neurotoxicity of environmental chemicals.Toxicology, 144: 121-127.
Andrade V, Mateus M, Batoreu M, Aschner M, dos Santos AM. 2015. Lead, arsenic, and manganese metal mixture exposures: focus on biomarkers of effect. Biol Trace Element Res, 166: 13-23.   
Bakhtiari N, Hosseinkhani S, Soleimani M, Hemmati R, Noori-Zadeh A, Javan M, Tashakor A. 2016. Short-term ursolic acid promotes skeletal muscle rejuvenation through enhancing of SIRT1 expression and satellite cells proliferation. Biomed Pharmacother, 78: 185-196.          
Bakhtiari N, Hosseinkhani S, Tashakor A, Hemmati R. 2015.Ursolic acid ameliorates aging-metabolic phenotype through promoting of skeletal muscle rejuvenation. Medical Hypotheses, 85: 1-6.            
Bencko V, Symon K. 1977. Test of environmental exposure to arsenic and hearing changes in exposed children. Environ Health Perspect, 19, 95.Chattopadhyay I, Biswas K, Bandyopadhyay U, Banerjee R K. 2004. Turmeric and curcumin: Biological actions and medicinal applications. CurrSci, 87: 44-53.       
Chen F, Wang H, Xiang X, Yuan J, Chu W, Xue X, Ge H, Zou M, Feng H, Lin J. 2014. Curcumin increased the differentiation rate of neurons in neural stem cells via wnt signaling in vitro study. J Sur Res, 192: 298-304.       
Dakeishi M, Murata K, Grandjean P. 2006.Long-term consequences of arsenic poisoning during infancy due to contaminated milk powder. Environ Health, 5, 1.  
Ellinsworth DC. 2015. Arsenic, reactive oxygen, and endothelial dysfunction. J Pharmacol Exp Ther, 353: 458-464.        
Fincher RME, Koerker R M. 1987. Long-term survival in acute arsenic encephalopathy.Follow-up using newer measures of electrophysiologic parameters. Am j med, 82: 549-552.        
Fujisawa S, Atsumi T, Ishihara M, Kadoma Y. 2004.Cytotoxicity, ROS-generation activity and radical-scavenging activity of curcumin and related compounds. Anticancer Res, 24: 563-570.         
Hill DS, Wlodarczyk BJ, Mitchell LE, Finnell RH. 2009.Arsenate-induced maternal glucose intolerance and neural tube defects in a mouse model. Toxicol  Appl Pharmacol, 239; 29-36.
Hiyama E, &Hiyama K. 2007.Telomere and telomerase in stem cells. Br J Cancer, 96;1020-1024.
Ho E. 2004. Zinc deficiency, DNA damage and cancer risk. J nutr biochem, 15;572-578.    
Hong GM, Bain LJ. 2012. Arsenic exposure inhibits myogenesis and neurogenesis in P19 stem cells through repression of the β-catenin signaling pathway. Toxicol Sci, 129;146-156.      
Hosseinzadehdehkordi M, Adelinik A, Tashakor A. 2015. Dual effect of curcumin targets reactive oxygen species, adenosine triphosphate contents and intermediate steps of mitochondria-mediated apoptosis in lung cancer cell lines. Eur J Pharmacol, 769; 203-210.     
Khan S, Vala JA, Nabi SU, Gupta G, Kumar D, Telang AG, Malik J. 2012. Protective effect of curcumin against arsenic-induced apoptosis in murine splenocytes in vitro. JImmunotoxicol, 9; 148-159.   
Kwon EB, Lee JY, Piao S, Kim IG, Ra JC,  Lee JY. 2011.Comparison of human muscle-derived stem cells and human adipose-derived stem cells in neurogenic trans-differentiation. Korean j urol, 52; 852-857.          
Li H, Engstrom K, Vahter M, Broberg, K. 2012. Arsenic exposure through drinking water is associated with longer telomeres in peripheral blood. Chem Res Toxicol, 25; 2333-2339.            
Lim JH, Boozer L, Mariani CL, Piedrahita JA, Olby NJ. 2010. Generation and characterization of neurospheres from canine adipose tissue-derived stromal cells. Cell Reprogram (Formerly" CloningStem Cells"), 12: 417-425.         
Menon VP, Sudheer AR. 2007. Antioxidant and anti-inflammatory properties of curcumin. The molecular targets and therapeutic uses of curcumin in health and disease, pp. 105-125: Springer.
Mukherjee S, Roy M, Dey S,  Bhattacharya RK. 2007.A mechanistic approach for modulation of arsenic toxicity in human lymphocytes by curcumin, an active constituent of medicinal herb Curcuma longa Linn. J Clin Biochem  Nutr, 41; 32-42.     
Muthumani M, Miltonprabu S. 2015. Ameliorative efficacy of tetrahydrocurcumin against arsenic induced oxidative damage, dyslipidemia and hepatic mitochondrial toxicity in rats. Chem Biol Interact, 235: 95-105.
Naujokas, M. F., Anderson, B., Ahsan, H., Aposhian, H. V., Graziano, J. H., Thompson C, Suk WA. 2013. The broad scope of health effects from chronic arsenic exposure: update on a worldwide public health problem. Environ Health Perspect (Online), 121:295.            
O'Sullivan RJ, Karlseder J. 2010. Telomeres: protecting chromosomes against genome instability. Nat rev Mol cell biol, 11: 171-181.          
Poojan S, Kumar S, Verma V, Dhasmana A, Lohani M, Verma MK. 2015.Disruption of Skin Stem Cell Homeostasis following Transplacental Arsenicosis: Alleviation by Combined Intake of Selenium and Curcumin. PloS One, 10: e0142818.  
Ramos O, Carrizales L, Yáñez L, Mejía J, Batres L, Ortíz D, Díaz-Barriga F. 1995. Arsenic increased lipid peroxidation in rat tissues by a mechanism independent of glutathione levels. Environ Health Perspect, 103: 85.         
Roy M, Sinha D, Mukherjee S,  Biswas, J. 2011. Curcumin prevents DNA damage and enhances the repair potential in a chronically arsenic-exposed human population in West Bengal, India. Eur J Cancer Prev, 20: 123-131.
SahabNegah S, Aligholi H, Khaksar Z, Kazemi H, Modarres Mousavi SM, Safahani M, BaratiDowom P, Gorji A. 2016 Survival, proliferation, and migration of human meningioma stem-like cells in a nanopeptide scaffold. Iranian Journal of Basic Medical Sciences 19:1271–1278.
SahabNegah S, Khaksar Z, Aligholi H, Mohammad Sadeghi S, Modarres Mousavi SM, Kazemi H, Jahanbazi Jahan-Abad A, Gorji A. 2016 Enhancement of Neural Stem Cell Survival, Proliferation, Migration, and Differentiation in a Novel Self-Assembly Peptide Nanofibber Scaffold. Mol Neurobiol,pp: 1-13.
Sankar P, Telang AG, Kalaivanan R, Karunakaran V, Suresh S, Kesavan M. 2016.Oral nanoparticulate curcumin combating arsenic-induced oxidative damage in kidney and brain of rats.Toxicol Ind Health, 32: 410-421.         
Sankar P, Telang AG, Ramya K, Vijayakaran  K, Kesavan M,  Sarkar SN. 2014.Protective action of curcumin and nano-curcumin against arsenic-induced genotoxicity in rats in vivo. Mol Biol Rep, 41: 7413-7422.         
Shankar S, Shanker U. 2014. Arsenic contamination of groundwater: A review of sources, prevalence, health risks, and strategies for mitigation. Scientific World J..          
Shi H, Hudson LG, Ding W, Wang S, Cooper KL, Liu S, Chen Y, Shi X, Liu KJ. 2004. Arsenite causes DNA damage in keratinocytes via generation of hydroxyl radicals. Chem Res Toxicol,  871-878.  
Smith AH, Lingas EO,  Rahman M. 2000. Contamination of drinking-water by arsenic in Bangladesh: a public health emergency. Bull World Health Organ, 78: 1093-1103.      
Stankowska DL, Krishnamoorthy VR, Ellis DZ, Krishnamoorthy, RR. 2015. Neuroprotective effects of curcumin on endothelin-1 mediated cell death in hippocampal neurons. Nutr Neurosci, 1-11.   
Tyler CR,  Allan AM. 2013. Adult hippocampal neurogenesis and mRNA expression are altered by perinatal arsenic exposure in mice and restored by brief exposure to enrichment. PloS One, 8: e73720.     
Xiao Z, Zhang A, Lin J, Zheng Z, Shi X, Di W, Fang Y. 2014. Telomerase: a target for therapeutic effects of curcumin and a curcumin derivative in Aβ1-42 insult in vitro. PloS One, 9: e101251.          
Zhang TC, Schmitt MT, Mumford JL. 2003. Effects of arsenic on telomerase and telomeres in relation to cell proliferation and apoptosis in human keratinocytes and leukemia cells in vitro. Carcinogenesis, 24: 1811-1817.
 
Avicenna Journal of Phytomedicine
Volume 7, Issue 4
July and August 2017
Pages 376-388

Files

Share

How to cite

Statistics