Antidiabetic, hypolipidemic and hepatoprotective effects of Arctium lappa root’s hydro-alcoholic extract on nicotinamide-streptozotocin induced type 2 model of diabetes in male mice

Document Type : Original Research Article

Authors

1 Health Research Institute, Diabetes Research Center, Department of Physiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

2 Department of Physiology, Health Research Institute, Diabetes Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Physiology, School of Medicine, Qom University of Medical Sciences, Qom, Iran

3 Department of Physiology, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

4 Department of Nutrition, Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

5 Health Research Institute, Diabetes Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Abstract

Objective: Arctium lappa (burdock), (A. lappa) root has hypoglycemic and antioxidative effects, and has been used for treatment of diabetes in tradition medicine. This study was conducted to evaluate the antidiabetic and hypolipidemic properties of A. lappa root extract on nicotinamide-streptozotocin (NA-STZ)-induced type2 diabetes in mice.
Materials and Methods: In this investigation, 70 adult male NMRI mice (30-35g) randomly divided into 7 groups (n=10) as follow: 1-control, 2-type 2 diabetic mice, 3-diabetic mice that received glibenclamide (0.25 mg/kg) as an anti-diabetic drug, 4, 5, 6 and 7- diabetic and normal animals that were pre-treated with 200 and 300 mg/kg A. lappa root extract, respectively, for 28 days. Diabetes has been induced by intraperitoneal injection of NA and STZ. Finally, the blood sample was taken and insulin, glucose, SGOT, SGPT, alkaline phosphatase, leptin and lipid levels was evaluated.
Results: Induction of diabetes decreased the level of insulin, leptin and high density lipoprotein (HDL) and increased the level of other lipids, glucose, and hepatic enzymes significantly (p<0.05). Administration of both doses of the extract significantly decreased the level of triglyceride, very low density lipoprotein, glucose and alkaline phosphatase in diabetic mice (p<0.05). Insulin levels increased in animals treated with 200 mg/kg (p<0.05) and HDL and leptin levels increased in animals treated with 300 mg/kg of the extract (p<0.01).
Conclusion: The results showed that A. lappa root extract, at specific doses, has an anti-diabetic effect through its hypolipidemic and insulinotropic properties. Hence, this plant extract may be beneficial in the treatment of diabetes.

Keywords

Main Subjects

References

 
Abbasnezhad AA, Hayatdavoudi P, Niazmand S, Mahmoudabady M. 2015. The effects of hydroalcoholic extract of Nigella sativa seed on oxidative stress in hippocampus of STZ-induced diabetic rats. Avicenna J Phytomed, 5: 333-340.
Ahangarpour A, Heidari H, Mard SA, Hashemitabar M, Khodadadi A. 2014. Progesterone and cilostazol protect mice pancreatic islets from oxidative stress induced by hydrogen peroxide. Iran J Pharm Res, 13: 937-944.
Ahangarpour A, Mohaghegh M, Asadinia E, Ramazani Ali-Akbari F. 2013. Effect of Arctium Lappa Root Extract on Glucose Levels and Insulin Resistance in Rats with High Sucrose Diet. J Shahid Sadoughi Uni, 21: 179-188. [Persian]
Ahangarpour A, Oroojan AA, Heidari H, Ehsan G, Rashidi Nooshabadi MR. 2014. Effects of Hydro-Alcoholic Extract of Rhus coriaria (Sumac) Seeds on Reproductive Complications of Nicotinamide-Streptozotocin Induced Type-2 Diabetes in Male Mice. World J Mens Health, 32: 151-158.
Ahangarpour A, Oroojan AA, Heidari H, Ghaedi E, Taherkhani R. 2015. Effects of Hydro-alcoholic Extract from Arctium lappa L. (Burdock) Root on Gonadotropins, Testosterone, and Sperm Count and Viability in Male Mice with Nicotinamide/ Streptozotocin-Induced Type 2 Diabetes. Malays J Med Sci, 22: 25-32.
Ahangarpour A, Oroojan AA, Ramezani Aliakbari F. 2014. Effects of C-peptide and Nicotinamide on serum LH, FSH, testosterone levels and sperm count in nicotinamide/STZ induced diabetic mice. Acta Endocrinologica (Buc), 4: 588-594.
Ahangarpour A, Teymuri Zamaneh H, Jabari A, Malekshahinia H, Heidari H. 2014. Antidiabetic and hypolipidemic effects of Dorema aucheri hydro alcoholic leave extract in streptozotocin-nicotinamide induced type 2 diabetes in male rats. Iran J Basic Med Sci, 17: 808-814.
Al-Shammaa DA, Saour KYM, Abdul-Khalik ZM. 2013. Phytochemical Investigation for the Main Active Constituents in Arctium lappa L. Cultivated in Iraq. Iraqi J Pharm Sci, 22: 18-24.
Andaloussi AB, Louis M, Vuong T, Meddah B, Madiraju P, Settaf A, Haddad PS. 2011. The in vivo antidiabetic activity of nigella sativa is mediated through activation of the AMPK pathway and increased muscle Glut4 content. Evid Based Complement Alternat Med, 538671: 1-9.
Cao J, Li C, Zhang P, Cao X, Huang T, Bai1 Y, Chen K. 2012. Antidiabetic effect of burdock (Arctium lappa L.) root ethanolic extract on streptozotocin-induced diabetic rats. Afr J Biotechnol, 11: 9079-9085.
Chander AP, Reddy RA, Puchchakayala G. 2011. Hypoglycemic and Antidiabetic Activity of Glochidion velutinum on Streptozotocin-Nicotinamide Induced Type 2 Diabetic Rats. Eur J BiolSci, 3: 126-130.
Chu Y, Gómez Rosso L, Huang P, Wang Z, Xu Y, Yao X. 2014. Liver Med23 ablation improves glucose and lipid metabolism through modulating FOXO1 activity. Cell Res, 24 (10): 1250-1265.
El-Soud NHA, Khalili MY, Hussein JS, Oraby FSH, Farrag AR. 2007. Antidiabetic Effects of Fenugreek Alkaliod Extract in Streptozotocin Induced Hyperglycemic Rats. Journal of Applied Science Research, 3(10): 1073-1083.
Ghamarian A, Abdollahi M, Su X, Amiri A, Ahadi A, Nowrouzi A. 2012. Effect of chicory seed extract on glucose tolerance test (GTT) and metabolic profile in early and late stage diabetic rats. Daru, 20(1): 56.
Hadjzadeh MA, Rajaei Z, Khodaei E, Malek M, Ghanbari H. 2016. Rheum turkestanicum rhizomes possess anti-hypertriglyceridemic, but not hypoglycemic or hepatoprotective effect in experimental diabetes. Avicenna J Phytomed, [In Press].
Horwich TB, Fonarow GC. 2010. Glucose, obesity, metabolic syndrome, and diabetes relevance to incidence of heart failure. J Am CollCardiol, 55: 283-293.
Indradevi S, Ilavenil S, Kaleeswaran B, Srigopalram S, Ravikumar S. 2012. Ethanolic extract of Crinum asiaticum attenuates hyperglycemia-mediated oxidative stress and protects hepatocytes in alloxan induced experimental diabetic rats. J King Saud Univ, 24(2): 171-177.
Kamat JP, Devasagayam TP. 1999. Nicotinamide (vitamin B3) as an effective antioxidant against oxidative damage in rat brain mitochondria. Redox Rep, 4(4): 179-184.
Kazemian Mansur Abad M, Haeri MR, Ebrahimi M, Heidari R. 2015. Anti-diabetic effect of Capparis spinosa L. root extract in diabetic rats. Avicenna J Phytomed, 5 (4): 325-332.
Li B, Lin W, Lin N, Dong X, Liu L. 2014. Study of the correlation between serum ferritin levels and the aggregation of metabolic disorders in non-diabetic elderly patients. Exp Ther Med, 7(6): 1671-1676.
Lin SC, Chung TC, Lin CC, Ueng TH, Lin YH, Lin SY, Wang LY. 2000. Hepatoprotective effects of Arctium lappa on carbon tetrachloride- and acetaminophen-induced liver damage. Am J Chin Med, 28(2): 163-173.
Lin SC, Lin CH, Lin CC, Lin YH, Chen CF, Chen IC, Wang LY. 2002. Hepatoprotective effects of Arctium lappa Linne on liver injuries induced by chronic ethanol consumption and potentiated by carbon tetrachloride. J Biomed Sci, 9: 401-409.
Long YC, Cheng Z, Copps KD, White MF. 2011. Insulin receptor substrates Irs1 and Irs2 coordinate skeletal muscle growth and metabolism via the Akt and AMPK pathways. Mol Cell Biol, 31(3): 430-441.
Ma Y, Wang Y, Huang Q, Ren Q, Chen S, Zhang A, Zhao L, Zhen Q, Peng Y. 2014. Impaired β Cell Function in Chinese Newly Diagnosed Type 2 Diabetes Mellitus with Hyperlipidemia. J Diabetes Res, 493039: 1-6.
Mousavi  SE,  Shahriari  A,  Ahangarpour  A, Vatanpour  H,  Jolodar  A.  2012. Effects  of  teucrium polium ethyl  acetate  extract  on  serum,  liver  and  muscle triglyceride  content  of  sucrose-induced  insulin resistance in rat. Iran J Pharm Res, 11: 347-355.
Ovalle-Magallanes B, Medina-Campos ON, Pedraza-Chaverri J, Mata R. 2015. Hypoglycemic and anti hyperglycemic effects of phytopreparations and limonoids from Swieteniahumilis. Phytochemistry, 110: 111-119.
Pandey M, Rastogi S, Singh Rawat A. 2007. Evaluation of Pharmacognostical Characters and Comparative Morphoanatomical Study of  Saussureacostus (Falc.) Lipchitz and Arctium lappa L. Roots. Natural Product Sciences, 13: 304-310.
Patel DK, Prasad SK, Kumar R, Hemalatha S. 2012. An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed, 2(4): 320-330.
Rafieian-Kopaei M, Shahinfard N, Rouhi-Boroujeni H, Gharipour M, Darvishzadeh-Boroujeni P. 2014. Effects of Ferulago angulata Extract on Serum Lipids and Lipid Peroxidation. Evid Based Complement Alternat Med, 2014: 680856.
Raju J, Gupta D, Rao AR, Yadava PK, Baquer NZ. 2001. Trigonellafoenum-graecum (fenugreek) seed powder improves glucose homeostasis in alloxan diabetic rat tissues by reversing the altered glycolytic, gluconeogenic and lipogenic enzymes. Mol Cell Biochem, 224: 45-51.
Rangachari B, Savarimuthu I. 2012. Antidiabetic and hypolipidemic effect of methanol extract of Lippianodiflora L. in streptozotocin induced diabetic rats. Asian Pac J Trop Biomed, 1: 1-7.
Raza H, John A. 2012. Streptozotocin-Induced Cytotoxicity, Oxidative Stress and Mitochondrial Dysfunction in Human Hepatoma HepG2 Cells. Int J MolSci, 13: 5751-5767.
Raza H, Prabu SK, John A, Avadhani NG. 2011. Impaired mitochondrial respiratory functions and oxidative stress in streptozotocin-induced diabetic rats. Int J MolSci, 12: 3133-3147.
Roghani M, VaezMahdavi MR, Jalali-Nadoushan MR, Baluchnejadmojarad T, Naderi G, Roghani-Dehkordi F, Taghi Joghataei M, Kord M. 2013. Chronic administration of daidzein, a soybean isoflavone, improves endothelial dysfunction and attenuates oxidative stress in streptozotocin-induced diabetic rats. Phytother Res, 27: 112-117.
Singh SK, Rai PK, Mehta S, Singh RK, Watal G. 2009. Curative effect of Cynodondactylon against STZ induced hepatic injury in diabetic rats. Indian J Clin Biochem, 24: 410-413.
Sohn EH, Jang SA, Joo H, Park S, Kang SC, Lee CH, Kim SY. 2011. Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L. ClinMol Allergy, 9: 1-11.
Song MY, Jeong GS, Kwon KB, Ka SO, Jang HY, Park JW, Kim YC, Park BH. 2010. Sulfuretin protects against cytokine-induced beta-cell damage and prevents streptozotocin-induced diabetes. Exp Mol Med, 42: 628-638.
Song Y, Manson JE, Buring JE, Sesso HD, Liu S. 2005. Associations of dietary flavonoids with risk of type 2 diabetes,  and  markers  of  insulin  resistance  and systemic inflammation in women: a prospective study and cross-sectional analysis. J Am Coll Nutr, 24: 376-384.
Whiting DR, Guariguata L, Weil C, Shaw J. 2011. IDF diabetes atlas: global estimates of the prevalence of diabetes for 2011 and 2030. Diabetes Res ClinPract, 94: 311-321.
Yilmaz O, Ersan Y, DilekOzsahin A, Ozturk AI, Ozkan Y. 2013. Consequences of the Combined α-tocopherol, Ascorbic Acid and α-lipoic Acid on the Glutathione, Cholesterol and Fatty Acid Composition in Muscle and Liver of Diabetic Rats. Iran J Basic Med Sci, 16: 165-172.
Zamami Y, Takatori S, Goda M, Koyama T, Iwatani Y, Jin X, Takai-Doi S, Kawasaki H. 2008. Royal jelly ameliorates insulin resistance in fructose-drinking rats. Biol Pharm Bull, 31: 2103-2107.
Zargari A. 1993. Medicinal Plants. vol 3; pp. 8-14, Tehran, Tehran University Publications. [Persian]
Zhang XF, Benny KH. 2000. Antidiabetic property of ethanolic extract of Andrographispaniculata in streptozotocin-diabetic rat. Acta Pharmacol Sin, 21: 1157-1164.
 
Avicenna Journal of Phytomedicine
Volume 7, Issue 2
March and April 2017
Pages 169-179

Files

Share

How to cite

Statistics