Antinociceptive effect of extracts of Marrubium astracanicum Jacq. aerial parts

Document Type : Original Research Article

Authors

1 Department of Pharmacognosy, Faculty of Pharmacy and Persian Medicine and Pharmacy Research center, Tehran University of Medical Sciences, Tehran, Iran

2 Departments of Pharmacognosy and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

3 Departments of Pharmacology and Toxicology and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

4 Faculty of Land and Food Systems, University of British Columbia, B.C., Canada

Abstract


Objective: The genus Marrubium is used for treatment of joint pain, gout, stomach-ache and colic in Iranian Traditional Medicine. Marrubium astracanicum Jacq. (M. astracanicum) is a native species in the flora of Iran. The aim of this study was to evaluate the antinociceptive properties of various extracts of aerial parts of M. astracanicum.

Materials and Methods: Antinociceptive activities of total hydroalcoholic extract (THE) and its n-hexane (non-polar) and residual partition (polar) fractions were analyzed using formalin test in mice. Morphine (5 mg/kg) and normal saline were used as positive and negative controls, respectively.

Results: Intraperitoneal administration of THE (50, 100 and 200 mg/kg), non-polar fraction (200 mg/kg) and polar fraction (100 and 200 mg/kg), 30 min before formalin injection, caused significant analgesic activity in acute phase (0-5 min after formalin injection) of formalin test (p0.05 in comparison with morphine).  In chronic phase (15–60 min after formalin injection), non-polar and polar fractions (50, 100 and 200 mg/kg) showed significant analgesic activity (p0.05 in comparison with morphine).

Conclusion: Different extracts of M. astracanicum demonstrated antinociceptive activity that support the traditional usage of Marrubium genus for the treatment of arthritis, gout and other inflammatory diseases.

Keywords

Main Subjects

References

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Avicenna Journal of Phytomedicine
Volume 7, Issue 1
January and February 2017
Pages 73-79

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