Evaluation of antimicrobial activity of Malus domestica fruit extract from Kashan area

Document Type : Short communication

Authors

1 Department of Hortiscience, Faculty of Agriculture and Natural Sources, Science and Research Branch, Islamic Azad University, Tehran, I. R. Iran

2 Essential Oils Research Institute, University of Kashan, Kashan, I. R. Iran

Abstract

Objective: Many species of plants present noticeable potency against human bacterial and fungal pathogens.
Materials and Methods: In the current study, antimicrobial activity of the fresh fruits of 4 cultivars (A to D) of Malus domestica cultivated in the Qamsar area of Kashan, Iranwas investigated. The disk diffusion and minimal inhibitory concentration (MIC) evaluation antimicrobial activity tests were performed.
Results: The samples showed moderate antimicrobial activities with inhibition zones from 11 to 16 mm in these tests. Cultivar D with inhibition zones of 16, 14, and 12 mm for E. coli, S. epidermidis and K. pneumoniae, respectively exhibited the best results in these tests. Cultivar A also showed a zone of inhibition of 11 mm against P. aerouginosa.
Conclusion: Moderate antimicrobial activities were observed for the studied apple cultivars.
 

Keywords


Alberto MR, Canavosio MAR, De nadra MCM. 2006. Antimicrobial effect of polyphenols from apple skins on human bacterial pathogens. Electronic J Biotechnol, 9: 3.
Alberto MR, Farías ME, Manca De Nadra, M. C. 2002. Effect of wine phenolic compounds on Lactobacillus hilgardii 5w viability. J Food Prot, 65: 211-213.
Alberto MR, Gómez-Cordovés C, Manca De Nadra MC. 2004. Metabolism of gallic acid and catechin by Lactobacillus hilgardii from wine. J Agri Food Chem, 52: 6465-6469.
Alberto MR, Farías ME, and Manca De Nadra MC. 2001. Effect of gallic acid and catechin on Lactobacillus hilgardii       5w growth and metabolism of organic compounds. J Agri Food Chem, 49: 4359-4363.
Ceylan E, Fung DYC, and Sabah JR. 2004. Antimicrobial activity and synergistic effect of cinnamon with sodium benzoate or potassium sorbate in controlling Escherichia coli O157:H7 in apple juice. J Food Sci, 69: 102-106.
Gulluce M, Sokmen M, Sahin F, Sokmen A, Adiguzel A, Ozer H. 2004 Biological activities of the essential oil and methanolic extract of Micromeria fruticosa (L) Druce ssp. Serpyllifolia (Bieb) PH Davis plants from the eastern Anatolia region of Turkey. J Sci Food Agric, 84: 735-741.
Harris LJ, Farber JN, Beuchat LR, Parish ME, Suslow TV, Garrett EH, et al. 2003. Outbreaks associated with fresh produce: Incidence, growth, and survival of pathogens in fresh and fresh-cut produce. In Comprehensive reviews in food science and food safety pp. 78-141. Iowa: Blackwell Publishing Professional.
Ingham SC, Schoeller EL, and Engel RA. 2006. Pathogen reduction in unpasteurized apple cider: Adding cranberry juice to enhance the lethality of warm hold and freeze–thaw steps. J Food Prot, 69: 293-298.
Jeong MR, Kim HY, and Jeong-Dan Cha JD. 2009. Antimicrobial activity of methanol extract from Ficus carica leaves against oral bacteria. J Bacteriol Virol, 39: 97 – 102.
Miller LG, and Kaspar CW. 1994. Escherichia coli O157:H7 acid tolerance and survival in apple cider. J Food Prot, 57: 460-464.
Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolke RH. 1995. Manual of clinical microbiology, 7th ed. Washington. DC: ASM. pp: 1773.
Raybaudi-Massilia       R, Mosqueda-Melgar J, and Martín-Belloso O. 2006. Antimicrobial activity of essential oils on Salmonella Enteritidis, Escherichia coli and Listeria innocua in fruit juices. J Food Prot. 69: 1579-1586.
Raybaudi-Massllia R, Osqueda-Melgar J, Martin-Belloso O. 2009. Antimicrobial activity of malic acid against Listeria monocytogenes, Salmonella enterritidis and Escherichia coli O157:H7 in apple, pear and melon juices. Food Control, 20: 105-112.
Rubnov S, Kashman Y, Rabinowitz R, Schlesinger M, Mechoulam R. 2001. Suppressors of cancer cell proliferation from fig (Ficus carica) resin: isolation and structure elucidation. J Nat Prod, 64: 993-996.
Ryu SR, Cho H, Jung JS, Jung ST. 1998. The study on the separation and antitumor activity as new substances in fig. J Applied Chem, 2: 961-964.
Shoji T, Akazome Y, Kanda T, Ikeda M, 2004. The toxicology and safety of apple polyphenolic extract. Food Chem Toxicol, 42: 959-967.
NCCLS (National Committee for Clinical Laboratory Standards). 1997. Performance Standards for Antimicrobial Disk Susceptibility Test, sixth ed. Approved Standard. M2-A6, Wayne, PA.
Vaya J, Mahmood S. 2006. Flavonoid content in leaf extracts of the fig (Ficus carica L.), carob (Ceratonia siliqua L.) and pistachio (Pistacia lentiscus L.). Biofactors, 28. 169-175.
Yigit D, yigit N, and Mavi A. 2009. Antioxidant and antimicrobial activities of  bitter and sweet apricot (Prunus armeniaca L.) kernels. Braz J Med Biol Res, 42: 346-352.
Xiangyang Qi. 2003. Study on antibacterial effect of apple-polyphenol extracts. Food Sci, 05.