Phytoremedial effect of Withania somnifera against arsenic-induced testicular toxicity in Charles Foster rats

Document Type : Original Research Article

Authors

Mahavir Cancer Institute & Research Centre, Patna, Bihar, India

Abstract

Objective: The main objective of the current study was to observe the ameliorative effect of Withania somnifera on arsenic-induced testicular toxicity by exploring the crucial parameters such as sperm counts, sperm motility, hormonal assay and lipid peroxidation including histopathology.
Materials and Methods: In the present study, arsenic in the form of sodium arsenite was administered orally to male Charles Foster rats for 45 days. Thereafter, ethanolic root extract of Withania somnifera was administered for 30 days to observe its ameliorative effect on male reproductive system.
Results: The study revealed that after administration of sodium arsenite, there was a decrease in the sperm counts and sperm motility accompanied by an increased incidence of sperm abnormalities and hormonal imbalance leading to infertility. However, after administration of Withania somnifera, there was significant reversal in the parameters denoting that it not only possesses antioxidant and rejuvenating property but also maintains the cellular integrity of testicular cells leading to normal functioning of it.
Conclusion: The study concludes that Withania somnifera possesses phytoremedial effect. It is one of the best antidotes against arsenic-induced reproductive toxicity.

Keywords

Main Subjects

References

Abdel-Magied EM, Abdel-Rahman HA, Harraz FM. 2001. The effect of aqueous extracts of Cynomorium coccineum and Withania somnifera on testicular development in immature Wistar rats. J Ethnopharmacol,75: 1-4.
 Ahmad MK, Mahdi AA, Shukla KK, Islam N, Rajender S, Madhukar D, Shankhwar SN, Ahmad S. 2010. Withania somnifera improves semen quality by regulating reproductive hormone levels and oxidative stress in seminal plasma of infertile males. Fertil Steril, 94: 989-996.
Ahmad, SA., Sayed M.H., Barua S, Khan MH., Faruquee MH, Jalil A, Hadi SA, Talukder HK. 2001. Arsenic in drinking water and pregnancy outcomes. Environ. Health Perspect, 109: 629–631.
Ali M, Khan SA, Dubey P, Nath A, Singh JK, Kumar R and Kumar A. 2013. Impact of arsenic on testosterone synthesis pathway and sperm production in mice. Inno J Med Health Sci. 4: 185-189.
API (Ayurvedic Pharmacopoeia of India). 2001. The Ayurvedic Pharmacopoeia of India, Part I, Volume I, Iedition. New Delhi (India): Government of India, Ministry of Health and Family Welfare, Department of Indian Systems of Medicine & Homoeopathy.
Aposhian HV, Aposhian MM. 2006 Arsenic Toxicology: Five Questions. Chem Res Toxicol,19: 1-60.
Aposhian HV, and MM Aposhian. 1989a. Newer developments in arsenic toxicity. J Am Coll Toxicol, 8: 1297-1305.
Aposhian HV. 1989b. Biochemical toxicology of arsenic. In: Reviews of Biochemistry and Toxicology (Eds: E. Hodgson, J.R. Bend and R.M. Philpot), 10: 265-299, New York, Elsevier.
Begum VH, Sadique J. 2004. Effect of Withania somnifera on glycosaminoglycan synthesis in carrageen in-induced air pouch granuloma. Biochem. Med. Metab. Biol, 38: 272-277.
Bhatnagar M, Sisodia SS, Bhatnagar R. 2005. Antiulcer and antioxidant activity of Asparagus racemosa WILLD and Withania somnifera DUNAL in rats. Ann N Y Acad Sci, 1056: 261-278.
Bhattacharya A, Ghosal S, Bhattacharya SK. 2001. Anti-oxidant effect of Withania somnifera glycowithanolides in chronic footshock stress induced perturbations of oxidative free radical scavenging enzymes and lipid peroxidation in rat frontal cortex and striatum. J Ethnopharmacol, 74: 1-6.
Bhattacharya SK, Muruganandam AV.2003. Adaptogenic activity of Withania somnifera: an experimental study using a rat model of chronic stress. Pharmacol Biochem Behav, 75: 547-555.
Bhattacharya SK, Satyan KS, Ghosal S. 1997. Antioxidant activity of glycowithanolides from Withania somnifera. Indian J Exp Biol, 35: 236-239.
Biswas NM, Roychowdhury G, and. Sarkar M. 1994. Effect of sodium arsenite on adrenocortical activities in male rats: Dose-duration dependent responses. Med Sci Res, 23: 153–154.
Chinoy NJ, Tewari K, and Jhala DD. 2004. Fluoride and/or arsenic toxicity in mice testis with formation of giant cells and subsequent recovery by some antidotes. Fluoride,37: 172–184.
DeSesso JM, Jacobson CF, Scialli AR, Farr CH, and Holson JF. 1998. An assessment of the developmental toxicity of inorganic arsenic. Reprod. Toxicol, 12: 385–433.
Dhuley JN. 1998. Effect of Ashwagandha on lipid peroxidation in stress-induced animals. J Ethnopharmacol, 60: 173-178.
Draper HH, Hadley M. 1990. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol, 186: 421-431.
Gupta SK, Dua A, Vohra BP. 2003. Withania somnifera (ashwagandha) attenuates antioxidant defense in aged spinal cord and inhibits copper induced lipid peroxidation and protein oxidative modifications. Drug Metabol Drug Interact,19: 211-222.
Hardy MP, Gao HB, Dong Q, Ge R, Wang Q, Chai WR, Feng X, and Sottas C. 2005.  Stress hormone and male reproductive function. Cell Tissue Res, 322: 147–153.
Ihrig MM, Shalat SL, and Baynes C.1998. A hospital-based case-control study of stillbirths and environmental exposure to arsenic using an atmospheric dispersion model linked to a geographical information system. Epidemiology, 9: 290–294.
Ilayperuma I, Ratnasooriya WD, Weerasooriya TR. 2002. Effect of Withania somnifera root extract on the sexual behaviour of male rats. Asian J Androl,4: 295-298.
Jayaprakasam B, Zhang Y, Seeram N, Nair M. 2003. Growth inhibition of tumor cell lines    by withanolides from Withania somnifera leaves. Life Sci, 74: 125-132.
Kabbaj O, Yoon SR, Holm C, Rose J, Vitale ML and Pelletier RM. 2003. Relationship of the hormone-sensitive lipase-mediated modulation of cholesterol metabolism in individual compartments of the testis to serum pituitary hormone and testosterone concentrations in a seasonal breeder, the mink (Mustela vison). Biol Reprod, 68: 722–734.
Kumar A, Ali M, Kumar R, Suman S, Kumar H, Nath A, Singh JK and Kumar D. 2013. Withania somnifera protects the haematological alterations caused by Sodium arsenite in Charles foster rats. Int J Res Ayurveda Pharm, 4: 491-494.
Kumar A, Ali M, Rahman MS, Iqubal A, Anand G, Niraj PK, and Kumar R. 2015. Antidote effect of plants of Himalayan sub- origin against arsenic induced toxicity. J Bio Chem Res, 2:99-109.                
Leblond, CP and Clermont Y. 1952. Definition of the stages of the cycle of the seminiferous epithelium in the rat. Ann N Y Acad Sci, 55: 548–573.
Milton AH, Smith W, Rahman B, Hasan Z, Kulsum U, Dear K, Rakibuddin M, and Ali A. 2005. Chronic arsenic exposure and adverse pregnancy outcomes in Bangladesh. Epidemiology, 16: 82–86.
Mukherjee A, Sengupta MK and Hossain MA. 2006. Arsenic contamination in groundwater: A global perspective with emphasis on the Asian Scenario. J Hlth Popul Nutri, 24: 142-163.
Mukherjee and Bhattacharya P. 2001. Arsenic in groundwater in the Bengal Delta Plain: slow poisoning in Bangladesh. Environ Rev, 9: 189-220.
Pant N, Kumar R, Murthy RC and Srivastava SP. 2001. Male reproductive effect of arsenic in mice. Biometals, 14: 113–117.
Pant N, Murthy RC, and Srivastava SP. 2004. Male reproductive toxicity of sodium arsenite in mice. Hum Exp Toxicol 23: 399–403.
Patil RB, Vora SR and Pillai MM. 2012. Protective effect of spermatogenic activity of Withania Somnifera (Ashwagandha) in galactose stressed mice. Annals of Biological Research 3: 4159-4165.
Qian Y, Castranova V and Shi XJ. 2003. New perspectives in arsenic-induced cell signal transduction. Inorg Biochem,96: 271-278.
Rasool M, Varalakshmi P. 2006. Immuno-modulatory role of Withania somnifera root powder on experimental induced inflammation: An in vivo and in vitro study. Vascul Pharmacol, 44: 406-410.
Reddy VBM, Reddy PS, Sasikala P, Reddy YVK. 2010. Transplacental and lactational exposure of arsenic to mice: Effect on steroidogenic enzymes and hormones of male reproduction. Int J Toxicol Pharmacol Res, 2: 95-98.
Rossman T. 2007. Arsenic. In: Rom W and Markowitz S eds. Environmental and occupational medicine, 4th ed. pp. 1006– 1017, Hagerstown, MD: Lippincott Williams & Wilkins.
Sarkar M, Chaudhuri GR, Chattopadhyay A, and Biswas NM. 2003. Effect of sodium arsenite on spermatogenesis, plasma gonadotrophins and testosterone in rats. Asian J Androl, 5: 27–31.
Shukla KK, Mahdi AA, Mishra V, Rajender S, Sankhwar SN, Patel D, and Das M. 2011. Withania somnifera improves semen quality by combating oxidative stress and cell death and improving essential metal concentrations. Reprod BioMed Online, 22: 421–427.
Singh AP, Goel RK, Kaur T. 2011. Mechanisms pertaining to arsenic toxicity. Toxicol Int, 18: 87-93.
Singh S and Rana SVS. 2007. Amelioration of arsenic toxicity by L-Ascorbic acid in laboratory rat. J Environ Bio, 28: 377-384. 
Sukh Dev. 2005. Prime Ayurvedic Plant Drugs. Tunbridge Wells (UK): Anshan.
Szinicz L. 1988. Forth W. Effect of As2O3 on gluconeogenesis. Arch Toxicol, 61: 444-449.
Tchounwou PB, Patlolla AK, and Centeno JA. 2003. Carcinogenic and systemic health effects associated with arsenic exposure—a critical review. Toxicol Pathol, 31: 575–588.
Upton R. 2000. editor. American Herbal Pharmacopoeia and Therapeutic Compendium: Ashwagandha Root (Withania somnifera) – Standards of Analysis, Quality Control, and Therapeutics. Santa Cruz (CA): American Herbal Pharmacopoeia.
Vaishali ND, Nilima UR, Dhar HL. 1987. Evaluation of antistress activity of Withania somnifera. Ind J Clin Bio, 2: 101-108.
Vreeburg, JT, Samaun K, Verkade HJ, Verhoef P, Ooms MP and Weber RF. 1988. Effects of corticosterone on the negative feedback action of testosterone, 5 alpha-dihydrotestosterone and estradiol in the adult male rat. J Steroid Biochem, 29: 93–98.
Wang A, Holladay SD, Wolf DC, Ahmad SA, Robertson JL. 2006. Reproductive and Developmental Toxicity of Arsenic in Rodents: A Review. Int J Toxicol, 25: 319–331.
Yang C & Frenkel K. 2002. Arsenic-mediated cellular signal transduction, transcription factor activation, and aberrant gene expression: implications in carcinogenesis, J Environ Pathol Toxicol Oncol,21: 331-342.
Avicenna Journal of Phytomedicine
Volume 5, Issue 4 - Serial Number 4
July and August 2015
Pages 355-364

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