The inhibitory effect of Thymus vulgaris extracts on the planktonic form and biofilm structures of six human pathogenic bacteria

Document Type : Original Research Article

Authors

Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.

Abstract

Objective: Microorganisms are responsible for many problems in industry and medicine because of biofilm formation. Therefore, this study was aimed to examine the effect of Thymus vulgaris (T. vulgaris) extracts on the planktonic form and biofilm structures of six pathogenic bacteria.
Materials and methods: Antimicrobial activities of the plant extracts against the planktonic form of the bacteria were determined using the disc diffusion method. MIC and MBC values were evaluated using macrobroth dilution technique. Anti-biofilm effects were assessed by microtiter plate method.
Results: According to disc diffusion test (MIC and MBC), the ability of Thymus vulgaris (T. vulgaris ) extracts for inhibition of bacteria in planktonic form was confirmed. In dealing with biofilm structures, the inhibitory effect of the extracts was directly correlated to their concentration. Except for the inhibition of biofilm formation, efficacy of each extract was independent from type of solvent.
Conclusion: According to the potential of Thymus vulgaris (T. vulgaris ) extracts to inhibit the test bacteria in planktonic and biofilm form, it can be suggested that Thymus vulgaris(T. vulgaris )extracts can be applied as antimicrobial agents against the pathogenic bacteria particularly in biofilm forms.

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References

Al-Rawi A, Chakravarty HL. 1988. Medicinal plants of Iraq. Ministry of Agriculture and Irrigation, Baghdad.
Androw JM. 2001. Standardized disc susceptibility testing method. J Antimicrob Chemother, 25:48-57.
Bauer AW, Kirby WM, Sherris JC, Truck N. 1996.Antimicrobial susceptibility testing by a standardized single disk method.Am J Clin Pathol,45:493-496.
Cowan MM. 1999.Plant products as antimicrobial agents.Clin Microbiol Rev, 12: 564–582.
Criado MT, Suarez B, Ferreros CM. 1994. The importance of bacterial adhesion in dairy industry. Food Technol, 48:123-126.
Diallo D, Hveem B, Mahmoud MA, Betge G, Paulsen BS, Maiga A. 1999.An ethnobotanical survey of herbal drugs of Gourma district, Mali. Pharm Biol, 37:80–91.
Dixon RA. 2001. Natural products and plant disease resistance. Nature, 411:843–847.
Dobre AA, Gagin V, Petru N. 2011.Antimicrobial activity of essential oils against food-borne bacteria evaluated by two preliminary methods. Rom Biotechol Lett, 16:119-125.
Flemming HC. 2002.Biofouling in water systems-cases, causes and counter measures. App Microbiol Biotechnol, 59:629-640.
Gonçalves GMS, Bottaro M, Nilson AC. 2011.Effect of the Thymus vulgaris essential oil on the growth of Streptococcus mutans.J Basic Appl Pharmaceutical Sci, 32:375-380.
Ismail MM, Essam TM, Mohamed AF, Mourad FE. 2012. Screening for the antimicrobial activities of alcoholic and aqueous extracts of some common spices in Egypt.Int J Microbiol Res, 3:200-207.
Jabra-Rizk MA, Meiller TF, James CE, Shirtliff ME. 2006. Effect of Farnesol on Staphylococcus aureus biofilm formation and antimicrobial susceptibility. Antimicrob Agents Chemother, 50:1463-1469.
Kavanaugh NL, Ribbeck K. 2012. Selected antimicrobial essential oils eradicate Pseudomonas spp. and Staphylococcus aureus biofilms. ApplEnvironMicrobiol, 78:4057-4061.
Kumar CG, Anand SK. 1998. Significance of microbial biofilms in food industry: A review. Int J Food Microbiol, 42:9-27.
McCann MT, Gilmore BF, Gorman SP. 2008.Staphylococcus epidermidisdevice related infections: pathogenesis and clinical management. J Pharm Pharmacol, 60:1551–1571.
Mohsenzadeh M. 2007. Evaluation of antibacterial activity of selected Iranian essential oil against Staphylococcus aureus and Escherichia coli in nutrient broth medium. Pak J Biol Sci, 10:3693-3697.
Motamedi H, Darabpour E, Gholipour M, SeyyedNejad SM. 2010. In vitro assay for the anti-brucella activity of medicinal plants against tetracycline-resistant Br. melitensis. J Zhejiang Univ-SciB (Biomed &Biotechnol), 11:506-511.
O'Toole GA, Kolter R. 1998.Flagellar and twitching motility are necessary for Pseudomonas aeruginosabiofilm development. Mol Microbiol, 30:295-304.
Parsek MR, Singh PK. 2003. Bacterial biofilms: an emerging link to disease pathogenesis. Annu Rev Microbiol, 57:677–701.
Priti V. 2012.Use of essential oils against gram negative pathogens. J Drug Deliv Therap, 2:134-137.
Ramage G, Lopez-Ribot JL. 2005. Techniques for antifungal susceptibility testing of Candida albicans biofilms. Methods Mol Med, 118:71–79.
Rizk AM. 1986. The phyto-chemistry of flora of Qatar. King Print of Richmond, Great Britian.
Rota MC, Herrera A, Martínez RM, Sotomayor JA, Jordán MJ. 2008. Antimicrobial activity and chemical composition of Thymus vulgaris, Thymus zygis and Thymus hyemalis essential oils. Food Control, 19: 681–687.
Sandasi M. 2008. The effect of plant extraction on microbial biofilm formation and development. Msc dissertation, Tshwane University of Technology.
Schlag S, Nerz C, Birkenstock TA, Altenberend F, Götz F. 2007.Inhibition of Staphylococcal biofilm formation by nitrite. J Bacteriol, 189:7911-7919.
Sedalackova P, Serovsky M, Horsakova I, Voldrich M. 2011.Cell Surface Characteristic of Asaiabogorensis – SpoilageMicroorganism of Bottled Water.Czech J Food Sci, 29:457–461.
Srinivasan D, Sangeetha N, Suresh T, Perumalsamy PL. 2001. Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. J Ethnopharmacol, 74:217–220.
 
Avicenna Journal of Phytomedicine
Volume 5, Issue 4 - Serial Number 4
July and August 2015
Pages 309-318

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