The efficacy of hydro alcoholic extract of Seidlitzia rosmarinus on experimental zoonotic cutaneous leishmaniasis lesions in murine model

Document Type : Original Research Article

Authors

1 Department of Parasitology and Mycology, Emam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences. Mashhad, I. R. Iran

2 Research Center for Skin Diseases & Cutaneous Leishmaniasis, School of Medicine, Mashhad University of Medical Sciences. Mashhad, I. R. Iran.

3 Centre for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, I. R. Iran.

4 Department of Pharmacology and Medicinal plant Research Center, School of Medicine, Mashhad University of Medical Science, Mashhad, I. R. Iran.

Abstract

Objective: Leishmaniasis is one of the most important parasitic infectious diseases in the world. Since last century, many efforts have been made to control and treat the disease, but appropriate vaccines, pesticides and medicines are not available or even eligible. The purpose of this study was to evaluate the effect of hydro-alcoholic extract of Seidlitzia rosmarinus on the lesions of experimental Cutaneous Leishmaniasis (CL) in Balb/c mice.
Materials and Methods: The population study was 60 Ballb/c mice which divided to 6 groups, all infected with Leishmania major [MRHO/75/IR]. Soon after the ulcer started to appear in the early stage, a dose of provided herbal extract with 5, 10 and 15% concentration applied on each lesion. The surface area of the lesions measured during an interval of 10 days. Direct Giemsa stained smears prepared two and four weeks after treatment.
Results: Increasing the mean size of the lesions was statistically significant compared to those in control group (p>0.001). Visceral Leishmaniasis (VL) developed in all of the mice including the control group that received Eucerine alone. Survival rate in group receiving 15% S. rosmarinus extracts showed significantly higher  compared to mice in control group (p<0.001).
Conclusion: Hydro-alcoholic extracts of S.rosmarinus with concentrations below15% did not show a therapeutic effect on experimental CL ulcers of Balb/c mice. Further studies with higher concentrations or nano particles are recommended.

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Main Subjects

References

Beheshti M, Ghotbi Sh, Amirizade S. 2007. Therapeutic and Adverse Effects of Glucantime Used for Treatment of Cutaneous Leishmaniasis. Shiraz E-Med. J, 8: 155-161.
Berman J. Current treatment approaches to Leishmaniasis. 2003. CurrOpin Infect Dis, 16:397-401.
Doroodgar A, Arbabi M, RazaviMR ,Mohebali M, Sadr F .2008. Treatment of Cutaneous Leishmaniasis in Murine Model by Hydro-alcoholic Essence of Artemisia sieberi. Journal of Arthropod-Borne Diseases, 2: 42-47.
Fata A, Elahi R., Haririzadeh G, Ghahramani S, Tavakoli 1993. Study the effect of juice, extract and active ingredient of Euphorbia myrsinites on growth of promastigotes in NNN medium. Med J Mashhad Univ Med.Sci., 36: 30-36
Fata A, Rakhshandeh H, Berenji F, Jalilianfard A. 2006. Treatment of Cutaneous Leishmaniasis in murine model by Alcoholic Extract of Berberis vulgaris. Iranian J Parasitol,1: 39-42.
Fatahi Bafghi A, Taghi Noorbala M , Hejazian SH. 2008. The Effect of RubiaTinctorium Extract on Cutaneous Leishmaniasis in BALB/c Mice. World J Zool, 3: 25-29.
Hadi MR. 2009. Biotechnological potentials of Seidlitziarosmarinus: A mini review. African Journal of Biotechnology, 8: 2429-2431.
Hedge IC, Akhani H, Freitag, H, Kother-Heinrich G, Podlech D, Rilke S, Uotila P. 1997. Flora Iranica. Chenopodiaceae.1997. pp. 172: 371, Graz, Akademische Druck-u. Verlagsanstalt, Austria
Jaafari M.R., Bavarsad N, FazlyBazzaz B.S., Samiei A., Dina Soroush, Ghorbani S., LotfiHeravi M.M., Khamesipour A. 2009. The Effect of Topical Liposomes Containing Paromomycin Sulfate (PM) in the Course of Leishmania major Infection in Susceptible BALB/c Mice. Antimicrob. Agents Chemother, 53: 2259-2265.
Jaafari M.R., Behravan J., BodaghAbadi A., Ramezani M. 2006. Evaluation of leishmanicidal effect of Euphorbia myrsinites extract by in vitro antileishmanial assay using promastigote of Leishmania. Major. Iranian J Basic Med Sc, 8: 295-298.
Kurban K. Iraqi advances in dermatology, news in dermatological treatment, 20 Sep. 2000, proceeding of 7th pan Arab Congress of Dermatology and Venereology, Tunis-Carthage, Tunisie.
Mirheydar H. 2001. Herbal Information: Usage of Plants in Prevention and Treatment of Diseases, Islamic Culture Press Center, Tehran, Iran.
Nakhaei M , Moin-Vaziri V , Fata A , Haghighi A , Iranshahi M , Abadi A.R , Sartavi K , Jaffari M.R , Effect of topical exudate and gel of Aloe vera in the course of leishmania major infection in susceptible Balb/C mice , 19Oct. 2010, 7thnational and the second regional congress of Parasitology and parasitic diseases in Iran, Tehran , Iran.
Nilforoushzadeh M A, Shirani-Bidabadi L A. 2008. Zolfaghari-Baghbaderania, S. Saberia, Siadata AH, Mahmoudib M. Comparison of Thymus vulgaris (Thyme), Achilleamillefolium (Yarrow) and propolis hydro-alcoholic extracts versus systemic glucantime in the treatment of cutaneous leishmaniasis in balb/c mice. J Vector Borne Dis, 45: 301–306.
Parsa A. 1959. Medicinal plants and drugs of plant origin in Iran. Plant Foods Hum Nutr, 5: 375-394.
Parsa A. 1960. Medicinal plants and drugs of plant origin in Iran. Plant Foods Hum Nutr, 7: 65-136.
Sabir M, Bhide NK. 1971. Study of some pharmacological actions of berberine. Indian J Physiol Pharm, 15: 111-32.
Tafaghodi M,Khamesipour A, Jaafari MR. 2011. Immunization against leishmaniasis by PLGA nanospheres encapsulated with autoclaved Leishmania major (ALM) and CpG-ODN. Parasitol Res, 108: 1265-1273.
Tafaghodi M., Khamesipour A., Jaafari M.R. 2010. Immunization against leishmaniasis by PLGA nanospheres loaded with an experimental Autoclaved Leishmania major (ALM) and Quillajasaponins. Tropical Biomedicine, 27: 639–650.
Weigel MM, Armijos RX. 2003. The traditional and medical treatment of cutaneous leishmaniasis in rural Ecuador. J Am Acad Dermatol, 48:893-96.
World Health Organization. 2006. Control of Leishmaniasis:Report by the Secretariat. Geneva.EB118/4.
 
Avicenna Journal of Phytomedicine
Volume 4, Issue 6 - Serial Number 6
November and December 2014
Pages 385-391

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