Evaluating the effects of galbanic acid on hydrogen peroxide-induced oxidative DNA damage in human lymphocytes

Document Type : Original Research Article

Authors

1 Department of Pharmacodynamy and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. Iran

2 Biotechnology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, I. R. Iran

3 Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mashhad University of Medical Sciences, I. R. Iran

4 Medical Toxicology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, I. R. Iran

Abstract

Objective: Ferula szowitsiana has been widely used for medicinal purposes around the world. The anti-oxidant effect of F.szowitsiana had been proved. The current study aims to determine the protective effects of galbanic acid, a sesquiterpene coumarin from F. szowitsiana, against hydrogen peroxide (H2O2) - induced oxidative DNA damage in human lymphocytes.
Materials and Methods: Human lymphocytes were incubated with H2O2 (0, 25, 50, 100, and 200 µM), galbanic acid (200 and 400 µM) and a combination of galbanic acid (200 and 400 µM) and H2O2 (25 µM) at 4 C for 30 minutes. Solvents of galbanic acid without H2O2 were used as negative controls.
Results: The findings of this study demonstrated that H2O2 exposure leads to a significant concentration-dependent increase in DNA damage. Galbanic acid did not cause DNA damage compared with the control cells. Data showed that galbanic acid does not have a protective effect against H2O2-induced oxidative DNA damage in human lymphocytes.
Conclusion: According to the results, it is concluded that the capability of F. szowitsiana in reducing reactive oxygen species and the anti-inflammatory property of its methanolic extract may be due to its other ingredients.

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References

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Avicenna Journal of Phytomedicine
Volume 4, Issue 5 - Serial Number 5
September and October 2014
Pages 337-342

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