Effects of ethanolic extract of green tea on decreasing the level of lipid profile in rat

Document Type : Original Research Article

Authors

Department of Pharmacology and Medicinal plant Research Center, School of Medicine, Mashhad University of Medical Science, Mashhad, I. R. Iran

Abstract

Objective: The aim of the present study was to elucidate the possible biochemical improving effect in lipid metabolic that may result from continuous treatment with Green Tea extract in normal albino rats and those rendered hyperlipidemic by long term supplementation of fat-enriched diet.
Materials and Methods: Fifty male albino rats aged six weeks with 200±10 g weight were randomly divided into five groups: Group A (negative control), groups B (positive control), Group C (treatment with drug), Group D (treatment with extract), and Group E (prevention with extract). All groups except Group A were received fat-enriched diet throughout the study. Group C received 25 mg/kg/day of the nicotinic acid from day 28 to the end of study. Group D was also treated with 100 mg/kg/day of the extract from day 28 to the end of study and finally group E was also treated with 100 mg/kg/day of the extract from the start of the study to the end. Lipid levels were determined weekly. Data were expressed as mean±SEM which were calculated using SigmaPlot® software. The obtained data were statistically analyzed using Student's t–test.
Results: In group (D), total cholesterol, LDLc, HDLc, and triglyceride levels were significantly decreased by 33.3%, 30.2%, 40%, respectively, compared with the group C (pConclusion: The results of this study demonstrated that the extract of green tea has a hyperlipidemic lowering effect.
 

Keywords

References

Austin MA .1989. Plasma triglyceride as a risk factor for coronary heart disease. Am J Epidemiol, 129: 249-259.
Cambien F, Jacquenson A, Richard JL, Warnet JM, Ducimetiere P, Claude JR.1986. Is the level of serum triglyceride a significant predictor of coronary death in “normocholesterolemic” subjects. The paris prospective study. Am J E pidemiol, 124: 624-632.
Chobanian AV. 1991. Single risk factor intervention may be inadequate to inhibit atherosclerosis progression when hypertension and hypercholesterolemic coexist. Hypertension,18: 130-131.
Dalessandri KM, Organ CH Jr. 1995. Surgery, drugs, lifestyle, and hyperlipidemia. Am J Surg, 169: 374-378.
Fujita H, Yokoyma K, Yoshikawa M. 2000. Classification and hypertensive activity of angiotensin I-converting enzyme inhibitory peptides derived from food protein. J Food Sci, 65: 564-569.
Fujita H, Yamagami T, Ohshima K. 2001. Long-term ingestion of a fermented soybean-derived touchi-extract with α-glucoside inhibitory activity is safe and effective in humans with borderline and mild type-2 diabetes. J Nutr, 131: 2105-2108.
 
Geleijnse JM, Launer LJ, Hofman A, Plos HA, Witteman JC. 1999. Tea flavonoids may protect against atherosclerosis: the Rotterdam study. Arch Int Med, 159: 2170-2174.
Guermani-Nicolle L, Villaume C, Bau HM, Schwertz A, Nicolas JP, Mejean L. 2001. The cholesterol-lowering property of soybean fed to rats is related to the fasting duration. Plant Foods Human Nutr, 56: 239-249.
Hertog MG, Feskens EJ, Hollman PC, Katan, MB, Kromhout D. 1993. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet, 342: 1007-1011.
Ishikawa T, Suzukawa M, Ito T, Yoshida H, Ayaori M, Nishiwaki M, Yonemura A, Hara Y, Nakamura H. 1997. Effect of tea flavonoid supplementation on the susceptibility of lowdensity lipoprotein to oxidative modification. Amer J Clin Nutr, 66: 261-266.
Kannel WB, Castelli WP, Gordon T, McNamara PM. 1971. Serum cholesterol, lipoproteins, and the risk of coronary heart disease. Ann Inter Med, 74: 1-12.
Keli SO, Hertog MG, Feskens EJ, Kromhout D. 1996. Dietary flavonoids, antioxidant vitamins and incidence of stroke: the Zutphen study. Arch Internal Med, 156: 637-642.
Kono S, Shinchi K, Ikeda N, Yanai F, and Imanishi K. 1992. Green tea consumption and serum lipid profiles: a cross-sectional study in Northern Kyushu, Japan. Prev Med, 21: 526-531.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Kumar AS, Mazumder A, Saravanan US. 2008. Antihyperlipidemic activity of Camellia Sinensis leaves in triton wr-1339 induced Albino rats. Pharmacol Magazn,4: 60-64.
Muramatsu  K, Fukuyo M, and Jara Y.1986 (a). Effect of green tea catechins on plasma cholesterol level in cholesterol-fed rats, J Nutr Sci Vitaminol, 32: 613-622.
Muramatsu K, Fukuyo M, and Hara Y.1986 (b). Effect of tea leaf catechin (-)-epigallocatechin gallate on plasma cholesterol level in rats. J Jpn Soc Nutr Food Sci, 39: 495-500.
Oliveros LB, Videla AM, Ramirez DC, Gimenez MS. 2003. Dietary fat saturation produces lipid modifications in peritoneal macrophage of mouse. J Nutr Biochem, 14: 370-377.
Rizvi F, Iftikhar M, George JP. 2003. Beneficial effects of fish liver preparations of sea bass (Lates calcarifer) versus gemfibrozil in high fat diet-induced lipid-intolerant rats. J Med Food, 6: 123-128.
Stensvold I, Tverdal A, Solvoll K, and Foss OP. 1992. Tea consumption, relationship to cholesterol, blood pressure, and coronary and total mortality. Prev Med, 4: 546-553.
Yoon M, Lee H, Jeong S, Kim JJ, Nicol CJ, Nam KW, Kim M, Cho BG, Oh GT. 2003. Peroxisome proliferatorsactivated receptor alpha is involved in the regulation of lipid metabolism by ginseng. Br J Pharmacol, 138: 11295- 1302.
Avicenna Journal of Phytomedicine
Volume 3, Issue 1 - Serial Number 1
November and December 2012
Pages 98-105

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