Protective effects of hydro-alcoholic extract of Quercus brantii against lead-induced oxidative stress in the reproductive system of male mice

Document Type : Original Research Article

Authors

1 Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Abstract

Objective: Exposure to heavy metals such as lead (Pb) results in oxidative stress induction in the male reproductive system. Herbal medicine can be utilized as antioxidant agents against oxidative stress. Quercus brantii (QB) has shown antioxidant activity in previous studies. The aim of the present study was to evaluate effects of QB hydro-alcoholic extract against Pb-induced oxidative stress in the male mice reproductive system.
Materials and Methods: Forty-two NMRI adult male mice were randomly divided into 7 groups of 6 animals each. Group I was the control group that received no treatment. Group II was the sham group and received 0.2 ml distilled water. Groups III and IV received QB hydro-alcoholic extract 500 and 1000 mg/kg bw, respectively. Group V received Pb 1000 ppm/kg bw. Group VI and VII received Pb 1000 ppm/kg bw and QB extract 500 and 1000 mg/kg bw, respectively. All groups received treatment via oral gavage. After 35 days, sperm parameters (i.e. sperm motility, count and morphology) were evaluated. Levels of sex hormones including LH, FSH, and testosterone, total antioxidant capacity (TAC), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured in animals’ serum.
 Results: Exposure to Pb negatively affected sperm parameters (i.e. sperm motility, count and morphology), decreased serum concentrations of sex hormones (i.e. LH, FSH, and testosterone), TAC and SOD activity but increased MDA levels. However, co-administration of 500 and 1000 mg/kg bw QB hydro-alcoholic extract and Pb considerably improved sperm parameters (i.e. sperm motility, count and morphology), increased sex hormones (i.e.LH, FSH, and testosterone), TAC, and SOD activity while decreased MDA levels in animals’ serum.
Conclusion: Administration of QB extracts (Low dose and high dose) is able to protect the male reproductive system of mice against Pb-induced oxidative stress.

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Main Subjects

References

Agarwal A, Virk G, Ong C, du Plessis SS. 2014. Effect of oxidative stress on male reproduction. World J Men Health, 32:1-7.
Hamadouche NA, NESRINE S, ABDELKEDER A. 2013. Lead toxicity and the hypothalamic-pituitary-testicular axis. Notulae Scientia Biologicae, 5:1-6.
Aitken RJ. 1995. Free radicals, lipid peroxidation and sperm function. Reproduction, Fertility and Development, 7:659-668.
Aitken RJ, Roman SD. 2008. Antioxidant systems and oxidative stress in the testes. Oxid Med Cell Longev, 1:15-24.
Al-Omair MA, Sedky A, Ali A, Elsawy H. 2017. Ameliorative Potentials of Quercetin against Lead-Induced Hematological and Testicular Alterations in Albino Rats. Chin J Physiol, 60:54-61.
Amin, GR. 1991. Popular medicinal plants of Iran, Vol. 1, pp. 40-41, Tehran, Iranian Research Institute of Medicinal Plants.
Anjum MR, Madhu P, Reddy KP, Reddy PS. 2017. The protective effects of zinc in lead-induced testicular and epididymal toxicity in Wistar rats. Toxicol Ind Health, 33:265-276.
njum, M. R., Sainath, S. B., Suneetha, Y., & Reddy, P. S. (2011). Ecotoxicology and Environmental Safety Lead acetate induced reproductive and paternal mediated developmental toxicity in rats $. Ecotoxicol Environ Saf, 74: 793–799.
Ayinde OC, Ogunnowo S, Ogedegbe RA. 2012. Influence of Vitamin C and Vitamin E on testicular zinc content and testicular toxicity in lead exposed albino rats. BMC Pharmacol Toxicol, 13:1-8.
Azizi S, Pirbalouti AG, Amirmohammadi M. 2014. Effect of hydro-alcoholic extract of Persian oak (Quercus brantii) in experimentally gastric ulcer. Iran J Pharm Res; 13:967-974.
Bajalan I, Javadian M, Zarinkoob S, Dalvand H. 2014. Antibacterial activity of the extracts of oak (Quercus persica) fruits. Bull Environ Pharmacol Life Sci; 3:62-65.
Benzie IF, Strain JJ. 1999. [2] Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol, 299:15-27.
Chowdhury AR. 2009. Recent advances in heavy metals induced effect on male reproductive function-A retrospective. Al Ameen J Med Sci, 2:37-42.
Çoruh N, Nebigil C, Özgökce F. 2014. Rapid and comprehensive separation for the phenolic constituents of Quercus brantii acorns by RP-HPLC-DAD. J Liq Chromatogr Relat Technol, 37:907-915.
Creasy DM. 2001. Pathogenesis of male reproductive toxicity. Toxicol Pathol, 29:64-76.
Dogan A, Celik I, Kaya MS. 2015. Antidiabetic properties of lyophilized extract of acorn (Quercus brantii Lindl.) on experimentally STZ-induced diabetic rats. J Ethnopharmacol, 176:243-251.
Dorostghoal M, Seyyednejad SM, Jabari A. 2014. Protective effects of Fumaria parviflora L. on lead‐induced testicular toxicity in male rats. Andrologia, 46:437-46.
El-Nekeety AA, El-Kady AA, Soliman MS, Hassan NS, Abdel-Wahhab MA. 2009. Protective effect of Aquilegia vulgaris (L.) against lead acetate-induced oxidative stress in rats. Food Chem Toxicol, 47:2209-22015.
Elgawish RA, Abdelrazek HM. 2014. Effects of lead acetate on testicular function and caspase-3 expression with respect to the protective effect of cinnamon in albino rats. Toxicol Rep, 1:795-801.
Fernandez CD, Bellentani FF, Fernandes GS, Perobelli JE, Favareto AP, Nascimento AF, Cicogna AC, Kempinas WD. 2011. Diet-induced obesity in rats leads to a decrease in sperm motility. Reprod Biol Endocrinol; 9:32.
Gandhi J, Hernandez RJ, Chen A, Smith NL, Sheynkin YR, Joshi G, Khan SA. 2017. Impaired hypothalamic-pituitary-testicular axis activity, spermatogenesis, and sperm function promote infertility in males with lead poisoning. Zygote, 25:103-110.
Kakkar P, Jaffery FN. Biological markers for metal toxicity. 2005. Environ Toxicol Pharmacol, 19:335-349.
Karimi A, Rafieian-Kopaei M, Moradi MT, Alidadi S. 2016. Anti–Herpes Simplex Virus Type-1 Activity and Phenolic Content of Crude Ethanol Extract and Four Corresponding Fractions of Quercus brantii L Acorn. J Evid Based Complementary Altern Med, 22:455-461.
Kemprinas W, Melo V, Santos A. 1990. Saturnism in male rat: Endocrine effects Braz J Med Biol Res, 23:1171-1175.
Kianoush S, Sadeghi M, Balali-Mood M. 2015. Recent Advances in the Clinical Management of Lead Poisoning. Acta Med Iran, 53:327.
Loraine JA, Bell ET. 1971. Hormone assays and their clinical application (No. 3rd Edition). Edinburgh, UK: E. & S. Livingstone.
Mabrouk A, Ben Cheikh H. 2016. Thymoquinone supplementation ameliorates lead-induced testis function impairment in adult rats. Toxicol Ind Health, 32:1114-11121.
Malecka A, Piechalak A, Tomaszewska B. 2009. Reactive oxygen species production and antioxidative defense system in pea root tissues treated with lead ions: the whole roots level. Acta Physiol Plant, 31:1053-1063.
Mangoli E, Talebi AR, Anvari M, Pourentezari M. 2013. Effects of experimentally-induced diabetes on sperm parameters and chromatin quality in mice. Iran J Reprod Med; 11:53-60.
Mohammadi J, Nikbakht J, Mohammadi B, Jafari Barmak M, Vafaienejad T, Talebianpoor MS. 2016. The healing effect of combined hydroalcoholic extract of Teocurium polium and the seed hull of Quercus brantii on burn wounds in rats. Int J Med Res  Health Sci, 5:232-237.
Mohammadzadeh A, Samadi-Maybodi A, Khodadoust S. 2013. Determination of trace elements in soil, leaves and fruits of Quercus brantii grown in southwestern Iran by atomic spectroscopy. Spectrochim Acta A Mol Biomol Spectrosc, 113:423-426.
Moradi MT, Karimi A, Alidadi S, Ghasemi-Dehkordi P, Ghaffari-Goosheh MS. 2016a. Cytotoxicity and in vitro antioxidant potential of Quercus Brantii acorn extract and the corresponding fractions. Int J Pharmacogn Phytochem Res, 8:558-562.
Moradi MT, Karimi A, Alidadi S. 2016b. In vitro antiproliferative and apoptosis-inducing activities of crude ethyle alcohole extract of Quercus brantii L. acorn and subsequent fractions. Chin J Nat Med, 14:196-202.
Naik GH, Priyadarsini KI, Satav JG, Banavalikar MM, Sohoni DP, Biyani MK, Mohan H. 2003. Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine. Phytochemistry, 63:97-104.
Niki E. 2008. Lipid peroxidation products as oxidative stress biomarkers. Biofactors, 34:171-80.
Nishikimi M, Rao NA, Yagi K. 1972. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun, 46:849-854.
Packer L, Weber SU, Rimbach G. 2001. Molecular aspects of α-tocotrienol antioxidant action and cell signalling. J Nutr, 131:369S-73S.
Pandya C, Pillai P, Nampoothiri LP, Bhatt N, Gupta S. 2012. Effect of lead and cadmium co‐exposure on testicular steroid metabolism and antioxidant system of adult male rats. Andrologia, 44(s1):813-22.
Patra RC, Rautray AK, Swarup D. 2011. Oxidative stress in lead and cadmium toxicity and its amelioration. Vet Med Int, 2011.
Placer ZA, Cushman LL, Johnson BC. 1966. Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal Biochem, 16:359-364.
Karimi A, Moradi MT, Saeedi M, Asgari S, Rafieian-Kopaei M. 2013. Antiviral activity of Quercus persica L.: high efficacy and low toxicity. Adv Biomed Res, 2:36.
Safary A, Motamedi H, Maleki S, Seyyednejad SM. 2009. A preliminary study on the antibacterial activity of Quercus brantii against bacterial pathogens, particularly enteric pathogens. Int J Botany, 5:176-180.
Sainath SB, Meena R, Supriya C, Reddy KP, Reddy PS. 2011. Protective role of Centella asiatica on lead-induced oxidative stress and suppressed reproductive health in male rats. Environ Toxicol Pharmacol, 32:146-154.
El Shafai A, Zohdy N, El Mulla K, Hassan M, Morad N. 2011. Light and electron microscopic study of the toxic effect of prolonged lead exposure on the seminiferous tubules of albino rats and the possible protective effect of ascorbic acid. Food Chem Toxicol, 49:734-743.
Sharma V, Kansal L, Sharma A. 2010. Prophylactic efficacy of Coriandrum sativum (Coriander) on testis of lead-exposed mice. Biol Trace Elem Res, 136:337-354.
Tahmouzi S. 2014. Optimization of polysaccharides from Zagros oak leaf using RSM: antioxidant and antimicrobial activities. Carbohydr Polym, 106:238-246.
Thoreux-Manlay A, Le Goascogne C, Segretain D, Jégou B, Pinon-Lataillade G. 1995. Lead affects steroidogenesis in rat Leydig cells in vivo and in vitro. Toxicology, 103:53-62.
World Health Organization. 2010. Department of Reproductive Health and Research. WHO Laboratory Manual for the Examination and Processing of Human Semen. 5:247-250.
Zhang H, Wei K, Zhang M, Liu R, Chen Y. 2014. Assessing the mechanism of DNA damage induced by lead through direct and indirect interactions. J Photochem Photobiol B, 136:46-53.
Avicenna Journal of Phytomedicine
Volume 8, Issue 5
September and October 2018
Pages 448-456

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