Renoprotective effects of the methanolic extract of Tanacetum parthenium against carbon tetrachloride-induced renal injury in rats

Document Type : Original Research Article

Authors

1 Department of Biochemistry, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

2 Department of Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

3 Department of Physiology, Ardabil University of Medical Sciences, Ardabil, Iran

4 Department of Health Information Technology Management, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

Abstract

Objective: Studies have demonstrated that carbon tetrachloride (CCl4) increases the generation of reactive oxygen species (ROS) in many tissues including the kidney, heart, lung, brain, and liver. The major aim of the present study was to evaluate the protective activity of Tanacetum parthenium extract (TPE) in renal tissues of CCl4-intoxicated rats.
Materials and Methods: Animals were divided into seven groups of six rats. Group 1 was the control group that was not treated with CCl4. The rats in the other groups were intraperitoneally injected with CCl4 (1.5 ml/kg, 1:1 in olive oil) on day 14. Rats in the groups bTPE40, bTPE80, and bTPE120 were gavaged with 40, 80, and 120 mg/kg of TPE, respectively for 14 constitutive days on a daily basis, before CCl4 administration. Rats in groups aTPE80 and aTPE120 were gavaged with 80 and 120 mg/kg of TPE, respectively, 2, 6, 24 and 48 hr after receiving CCl4. Blood samples were collected at the end of the 16th day through an intracardiac puncture and then serums were separated.
Results: CCl4 increased urea, creatinine, uric acid and creatinine: albumin (C/A) ratio level in serum and decreased total antioxidant and antioxidant enzymes (SOD and GPx) when compared to the control group (p<0.001). But administration of TPE to rats either before or after exposure to CCl4, attenuated these changes when compared with CCl4 control group (p Conclusion: TPE had potent nephroprotective effects against oxygen free radicals produced through CCl4 metabolism.

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References

Aksoy L, Sözbilir NB. 2012. Effects of Matricaria chamomilla L. on lipid peroxidation, antioxidant enzyme systems, and key liver enzymes in CCl4-treated rats. Toxicol Environ Chem, 94: 1780-1788.
Benzie IF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem, 239: 70-76.
Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 72: 248-254.
El-mohsen Ali SA, Abdelaziz D. 2014. The Protective Effect of Date Seeds on Nephrotoxicity Induced by Carbon Tetrachloride in Rats. Int J Pharm Sci Rev Res, 26: 62-68.
Ganie SA, Haq E, Hamid A, Qurishi Y, Mahmood Z, Zargar BA, Masood A, Zargar MA. 2011. Carbon tetrachloride induced kidney and lung tissue damages and antioxidant activities of the aqueous rhizome extract of Podophyllum hexandrum. BMC Complement Altern Med, 28: 11-17.
Hussain L, Akash MSH, Tahir M, Rehman K, Ahmed KZ. 2014. Hepatoprotective effects of methanolic extract of Alcea rosea against acetaminophen-induced hepatotoxicity in mice. Bangladesh J Pharmacol, 9: 322-327
Jain NK, Kulkarni SK. 1999. Antinociceptive and anti-inflammatory effects of Tanacetum parthenium L. extract in mice and rats. J Ethnopharmacol, 68: 251-259.
Khan RA, Khan MR, Sahreen S, Bokhari J. 2010. Prevention of CCl4-induced nephrotoxicity with Sonchus asper in rat. Food Chem Toxicol, 48: 2469-2476.
Mahmoodzadeh Y, Mazani M, Rezagholizadeh L. 2017. Hepatoprotective Effect of Methanolic Tanacetum Parthenium Extract on CCl4-Induced Liver Damage in Rats. Toxicol Rep, 30:455-462
Mallhi TH, Abbas K, Ali M, Qadir MI, Saleem M, Khan YH. 2014. Hepatoprotective activity of methanolic extract of Malva parviflora against paracetamol-induced hepatotoxicity in mice. Bangladesh J Pharmacol, 9: 342-346.
Marimuthu S, Adluri RS, Rajagopalan R, Menon VP. 2013. Protective role of ferulic acid on carbon tetrachloride-induced hyperlipidemia and histological alterations in experimental rats. J Basic Clin Physiol Pharmacol, 24: 59-66.
Olagunju JA, Adeneye AA, Fagbohunka BS, Bisuga NA, Ketiku AO, Benebo AS, Olufowobi OM, Adeoye AG, Alimi MA, Adeleke AG. 2009. Nephroprotective activities of the aqueous seed extract of Carica papaya Linn. In carbon tetrachloride induced renal injured Wistar rats: a dose-and time-dependent study. Biol Med, 1: 11-19.
Pareek A, Suthar M, Rathore GS, Bansal V. 2011. Feverfew (Tanacetum parthenium L.): A systematic review. Pharmacogn Rev, 5: 103-110.
Pourfarjam Y, Rezagholizadeh L, Nowrouzi A, Meysamie A, Ghaseminejad S, Ziamajidi N, Norouzi D. 2017. Effect of Cichorium intybus L. seed extract on renal parameters in experimentally induced early and late diabetes type 2 in rats. Ren Fail, 39: 211-221.
Rateb MEM, El-Gendy AAM, El-Hawary SS, El-Shamy AM. 2008. Phytochemical and biological studies on the different organs of Tanacetum parthenium L. cultivated in Egypt. Journal of Medicinal Plants, 1: 8-22.
Sahreen S, Khan MR, Khan RA, Alkreathy HM. 2015. Protective effects of Carissa opaca fruits against CCl4-induced oxidative kidney lipid peroxidation and trauma in rat. Food Nutr Res, 59: 10.3402/fnr.v59.28438
Shafaghat A, Salimi F. 2008. Extraction and determining of chemical structure of flavonoids in Tanacetum parthenium (L.) Schultz. Bip. From Iran. J Sci I A U, 18: 39-42.
Sharopov FS, Setzer WN, Isupov SJ, Wink M. 2015. Composition and bioactivity of the essential oil of Tanacetum parthenium from a wild population growing in Tajikistan. Am J Essent Oils Nat Prod, 2: 32-34.
Uchiyama M, Mihara M. 1978. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem, 86: 271-278.
Venkatanarayana G, Sudhakara G, Sivajyothi P, Indira P. 2012. Protective effects of curcumin and vitamin E on carbon tetrachloride-induced nephrotoxicity in rats. EXCLI J, 11: 641-650.
Vuda M, D'Souza R, Upadhya S, Kumar V, Rao N, Kumar V, Boillat C, Mungli P. 2012. Hepatoprotective and antioxidant activity of aqueous extract of Hybanthus enneaspermus against CCl4-induced liver injury in rats. Exp Toxicol Pathol, 64: 855-859.
Zeinali M, Rezaee SA, Hosseinzadeh H. 2017. An overview on immunoregulatory and anti-inflammatory properties of chrysin and flavonoids substances. Biomed Pharmacother, 92: 998-1009.
Zeinali M, Tayebi MN, Rezaee SA, Rafatpanah H, Hosseinzadeh H. 2017. Protective effects of chrysin on sub-acute diazinon-induced biochemical, hematological, histopathological alterations, and genotoxicity indices in male BALB/c mice. Drug Chem Toxicol, 2: 1-11.
Zirak MR, Rahimian R, Ghazi-Khansari M, Abbasi A, Razmi A, Mehr SA, K. Mousavizadeh K, Dehpour AR. 2014. Tropisetron attenuates cisplatin-induced nephrotoxicity in mice. Eur J Pharmacol, 738: 222-229.
Avicenna Journal of Phytomedicine
Volume 8, Issue 4
July and August 2018
Pages 370-379

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